Construct: ORF TRCN0000478348
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001127.1_s317c1
- Derived from:
- ccsbBroadEn_02660
- DNA Barcode:
- ACAAATTCCGAGATAGACGTCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCTN3 (11258)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478348
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11258 | DCTN3 | dynactin subunit 3 | NM_024348.4 | 100% | 100% | |
| 2 | human | 11258 | DCTN3 | dynactin subunit 3 | NM_001281426.2 | 87.5% | 87.5% | 408_409ins66 |
| 3 | human | 11258 | DCTN3 | dynactin subunit 3 | NM_007234.5 | 83.4% | 74.3% | (many diffs) |
| 4 | human | 11258 | DCTN3 | dynactin subunit 3 | NM_001281427.2 | 81.6% | 68.7% | (many diffs) |
| 5 | human | 11258 | DCTN3 | dynactin subunit 3 | NM_001281425.1 | 68.9% | 60% | (many diffs) |
| 6 | mouse | 53598 | Dctn3 | dynactin 3 | NM_001159565.1 | 76.6% | 70.2% | (many diffs) |
| 7 | mouse | 53598 | Dctn3 | dynactin 3 | NM_016890.4 | 75.9% | 70.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggtctgact gacttgcagc ggctacaggc ccgagtggaa gagctggagc 121 gctgggtgta cgggccgggc ggggcgcgcg gctcacggaa ggtggctgac ggcctggtca 181 aggtgcaggt ggctttgggg aacatttcca gcaagaggga gagggtgaag attctctaca 241 aaaagattga agatctgatc aagtacctgg aTCCTGAGTA CATCGACCGC ATTGCCATAC 301 CTGATGCCTC TAAGCTGCAA TTCATCCTAG CAGAGGAGCA GTTTATCCTT TCCCAGGTTG 361 CACTCCTGGA GCAGGTGAAT GCCTTGGTGC CCATGCTGGA CAGTGCTCAC ATCAAAGCCG 421 TTCCTGAGCA TGCTGCCCGC CTGCAGCGCT TGGCCCAGAT CCACATTCAG CAGCAGGCTC 481 CATGGGGAGT GGGAGTCCGT GATGAGGCAG GAAGTTTAGT GGAAGATGTG GGCTTTGCCC 541 AGTTCCTTTC TGTGCTACAC TTTGGCCCTA CAGGACCAGT GTGTGGAAAT CACTGCCCAA 601 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 661 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 721 TATCTTGTGG AAAGGACGAA CAAATTCCGA GATAGACGTC CAAACGCGTT AAGTCgacaa 781 tcaacctctg gattacaaaa tttgtgaaag att