Transcript: Human NM_001281425.1

Homo sapiens dynactin subunit 3 (DCTN3), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
DCTN3 (11258)
Length:
773
CDS:
60..536

Additional Resources:

NCBI RefSeq record:
NM_001281425.1
NBCI Gene record:
DCTN3 (11258)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001281425.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000108247 GCAGGACCAGTGTGTGGAAAT pLKO.1 383 CDS 100% 10.800 14.040 N DCTN3 n/a
2 TRCN0000300959 GCAGGACCAGTGTGTGGAAAT pLKO_005 383 CDS 100% 10.800 14.040 N DCTN3 n/a
3 TRCN0000108246 TGATGCCTCTAAGCTGCAATT pLKO.1 296 CDS 100% 10.800 7.560 N DCTN3 n/a
4 TRCN0000300895 TGATGCCTCTAAGCTGCAATT pLKO_005 296 CDS 100% 10.800 7.560 N DCTN3 n/a
5 TRCN0000108245 CTTTGTTACAAGGCAGAGGAA pLKO.1 600 3UTR 100% 2.640 1.848 N DCTN3 n/a
6 TRCN0000300957 CTTTGTTACAAGGCAGAGGAA pLKO_005 600 3UTR 100% 2.640 1.848 N DCTN3 n/a
7 TRCN0000108248 GATCTGATCAAGTACCTGGAT pLKO.1 246 CDS 100% 2.640 1.848 N DCTN3 n/a
8 TRCN0000300896 GATCTGATCAAGTACCTGGAT pLKO_005 246 CDS 100% 2.640 1.848 N DCTN3 n/a
9 TRCN0000108249 CAATGCTTCTCTCCAAGCAAT pLKO.1 448 CDS 100% 0.495 0.347 N DCTN3 n/a
10 TRCN0000300956 CAATGCTTCTCTCCAAGCAAT pLKO_005 448 CDS 100% 0.495 0.347 N DCTN3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001281425.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02660 pDONR223 100% 68.9% 60% None (many diffs) n/a
2 ccsbBroad304_02660 pLX_304 0% 68.9% 60% V5 (many diffs) n/a
3 TRCN0000478348 ACAAATTCCGAGATAGACGTCCAA pLX_317 60.7% 68.9% 60% V5 (many diffs) n/a
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