Construct: ORF TRCN0000478392

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007000.5_s317c1
Derived from:
ccsbBroadEn_03932
DNA Barcode:
AACGCGCACTTCGTACGTGTCAAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
C17orf75 (64149)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478392
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64149 C17orf75 chromosome 17 open reading ... NM_022344.4 100% 100%
2 human 64149 C17orf75 chromosome 17 open reading ... XM_005258022.4 97.9% 97.9% 0_1ins24
3 human 64149 C17orf75 chromosome 17 open reading ... XM_017024940.2 54.7% 54.7% 0_1ins537
4 human 64149 C17orf75 chromosome 17 open reading ... XM_017024941.2 54.7% 54.7% 0_1ins537
5 mouse 70591 5730455P16Rik RIKEN cDNA 5730455P16 gene NM_027472.3 88.6% 89.1% (many diffs)
6 mouse 70591 5730455P16Rik RIKEN cDNA 5730455P16 gene XM_006534198.2 57.1% 58% (many diffs)
7 mouse 70591 5730455P16Rik RIKEN cDNA 5730455P16 gene XM_006534199.3 57.1% 58% (many diffs)
8 mouse 70591 5730455P16Rik RIKEN cDNA 5730455P16 gene XM_006534197.3 56.9% 50.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1254
ORF length:
1188
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct cccctctttg caggagtcga tggatggaga tgaaaaggaa ctagagagca
121gcgaagaggg aggctcagcc gaggagcgga gactcgagcc gccgtccagc agccactact
181gtctttacag ctatcgcgga agcagattgg cacagcaacg aggggacagt gaggacggaa
241gcccaagtgg cacaaatgca gaaactccct ctggtgatga tttcagcctc tccttggcag
301atactaatct accatccgaa gtggagccag agctgcgcag tttcattgct aagcgtcttt
361caagaggtgc agtctttgaa gggctgggta atgttgcatc tgtggagcta aaaattccag
421gttaccgagt tggttgttat tactgccttt tccaaaatga aaaactgctt cctgaaacag
481taacgataga ctctgaacgt aacccttcag aatatgtggt ctgtttttta ggagggtctg
541aaaaaggact tgagcttttc aggcttgaat tggacaagta cattcaaggg ctgaaaaata
601acatgaattg tgaggcaagg ggcctggaga gtcacataaa atcctatctg agcagctggt
661ttgaggatgt tgtatgccca atccaaaggg ttgttcttct ctttcaggaa aagcttacct
721ttctgctaca tgctgctttg agttacactc ctgttgaagt taaagaatca gatgaaaaaa
781caaagagaga cattaacagg tttctgagtg tggccagtct tcaaggactt attcatgaag
841gcaccatgac ttctttgtgc atggccatga cagaggagca gcataagtct gtggtcatcg
901attgcagcag ctcccagccT CAGTTCTGCA ATGCAGGAAG TAACCGGTTT TGTGAGGATT
961GGATGCAAGC TTTTTTAAAT GGTGCCAAAG GAGGTAACCC TTTTCTTTTC CGACAAGTAC
1021TGGAGAACTT TAAACTAAAG GCCATACAAG ACACAAACAA TTTGAAGAGA TTTATCCGAC
1081AGGCAGAAAT GAATCATTAT GCTTTGTTTA AATGTTACAT GTTCCTAAAG AACTGTGGTA
1141GTGGAGATAT ACTTTTGAAG ATTGTTAAAG TGGAACATGA AGAAATGCCT GAAGCCAAAA
1201ATGTGATAGC TGTCCTTGAA GAATTCATGA AAGAAGCTCT TGACCAAAGT TTTTGCCCAA
1261CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1321ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1381TATCTTGTGG AAAGGACGAA ACGCGCACTT CGTACGTGTC AAGACGCGTT AAGTCgacaa
1441tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)