Construct: ORF TRCN0000478426
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002202.1_s317c1
- Derived from:
- ccsbBroadEn_11412
- DNA Barcode:
- GGGAGGCTCAGTTTGATGTATATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAPGEF5 (9771)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478426
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9771 | RAPGEF5 | Rap guanine nucleotide exch... | NM_001367600.2 | 76.3% | 76.3% | (many diffs) |
| 2 | human | 9771 | RAPGEF5 | Rap guanine nucleotide exch... | XM_011515652.2 | 68.6% | 64.2% | (many diffs) |
| 3 | human | 9771 | RAPGEF5 | Rap guanine nucleotide exch... | NM_001367601.1 | 67.9% | 61.6% | (many diffs) |
| 4 | human | 9771 | RAPGEF5 | Rap guanine nucleotide exch... | XM_005249916.1 | 57.9% | 53% | (many diffs) |
| 5 | human | 9771 | RAPGEF5 | Rap guanine nucleotide exch... | NM_001367602.2 | 57% | 57% | (many diffs) |
| 6 | human | 9771 | RAPGEF5 | Rap guanine nucleotide exch... | XM_017012837.2 | 55.7% | 51.1% | (many diffs) |
| 7 | human | 9771 | RAPGEF5 | Rap guanine nucleotide exch... | NM_001367603.1 | 55.6% | 54.9% | (many diffs) |
| 8 | human | 9771 | RAPGEF5 | Rap guanine nucleotide exch... | NM_012294.5 | 47.9% | 43.8% | (many diffs) |
| 9 | mouse | 217944 | Rapgef5 | Rap guanine nucleotide exch... | NM_175930.5 | 47.2% | 43.2% | (many diffs) |
| 10 | mouse | 217944 | Rapgef5 | Rap guanine nucleotide exch... | XM_006515781.3 | 41.7% | 38.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1398
- ORF length:
- 1332
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cagctcccgg ctgagggtct ttgaccctca tttggagagg aaagattccg 121 ccgcggcgct ctcagaccga gagctgccct tgcctacctt cgatgtgcct tatttcaaat 181 acatcgacga ggaggatgag gacgatgaat ggagcagccg ctcgcagtct tccaccgagg 241 atgactcagt ggactctctg ctctctgaca gatatgtggt ggtgtccggg accccggaga 301 agattttgga gcaccttttg aatgacttgc acctggaaga agtccaggac aaagaaacag 361 agaccctcct ggatgacttc cttctcacgt acactgtctt catgacaact gatgacttgt 421 gccaggctct gttaaggcac tattctgcta agaagtatca aggcaaagag gaaaactcag 481 atgttccgcg taggaaacgt aaagtcttgc atcttgtttc ccagtggatt gctctgtaca 541 aagactggtt acctgaagat gaacattcaa aaatgttttt aaagaccata tataggaatg 601 tactggatga tgtttatgaa tatccaatac ttgaaaaaga attgaaagaa tttcaaaaga 661 tacttggaat gcaccgtcgt cacactgtag atgaatattc accacaaaaa aagaataaag 721 cccttttcca ccaattcagt cttaaggaga actggctcca gcatagagga actgtgactg 781 aaacggagga aattttctgc cacgtgtata taacagagca ctcctatgtc agtgtgaagg 841 caaaagtttc cagtatagcc caagagattc tgctctgcag ccagctgggc aagcgagtgc 901 agctggtgaa aaaattcatc aaaattgcgg ctcactgcaa agcccagaga aacctgaatt 961 ctttctttgc cattgtgatg ggtctcaaca ctgcttctgt cagtcgactg tcgcagacct 1021 gggagaaaat ccctgggaag tttaagaaac ttttctctga acttgaaagt ttaacagatc 1081 cttccctaaa TCACAAAGCC TACAGAGATG CATTCAAAAA GATGAAGCCA CCAAAAATCC 1141 CTTTCGTGCC CTTATTGCTT AAAGATGTAA CATTTATTCA TGAAGGAAAT AAAACTTTTT 1201 TGGATAATCT TGTCAATTTT GAAAAGCTGC ATATGATCGC AGACACTGTC CGAACCCTGA 1261 GACACTGCAG GACTAACCAG TTTGGTGACC TGTCTCCAAA AGAGCATCAA GAGTTAAAGT 1321 CCTATGTTAA TCACCTGTAT GTCATTGACA GCCAGCAGGC TCTGTTTGAG CTCTCACACA 1381 GGATCGAGCC GCGGGTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1441 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1501 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGGGAGGC TCAGTTTGAT 1561 GTATATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt