Transcript: Mouse XM_006515781.3

PREDICTED: Mus musculus Rap guanine nucleotide exchange factor (GEF) 5 (Rapgef5), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Rapgef5 (217944)
Length:
6458
CDS:
34..2802

Additional Resources:

NCBI RefSeq record:
XM_006515781.3
NBCI Gene record:
Rapgef5 (217944)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006515781.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000109996 GCAAGGTCTTACATCTGGTTT pLKO.1 1490 CDS 100% 4.950 6.930 N Rapgef5 n/a
2 TRCN0000109998 GCACTCCTATATCAGTGTGAA pLKO.1 1809 CDS 100% 4.950 6.930 N Rapgef5 n/a
3 TRCN0000109995 CCAGTCCAAGTTTCAGCTTAT pLKO.1 3064 3UTR 100% 10.800 7.560 N Rapgef5 n/a
4 TRCN0000109997 GCCGTCACACTGTTGATGAAT pLKO.1 1667 CDS 100% 5.625 3.938 N Rapgef5 n/a
5 TRCN0000109999 CAGAAGAAGAATAAAGCCCTT pLKO.1 1696 CDS 100% 2.160 1.512 N Rapgef5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006515781.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11412 pDONR223 100% 41.7% 38.4% None (many diffs) n/a
2 ccsbBroad304_11412 pLX_304 0% 41.7% 38.4% V5 (many diffs) n/a
3 TRCN0000478426 GGGAGGCTCAGTTTGATGTATATC pLX_317 23.5% 41.7% 38.4% V5 (many diffs) n/a
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