Construct: ORF TRCN0000478429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007446.1_s317c1
- Derived from:
- ccsbBroadEn_04192
- DNA Barcode:
- ACCCAACCAGGGGATCCGATGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COQ10B (80219)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478429
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80219 | COQ10B | coenzyme Q10B | NM_025147.4 | 100% | 100% | |
| 2 | human | 80219 | COQ10B | coenzyme Q10B | NM_001320819.1 | 84.7% | 86.1% | 0_1ins97;5G>C;7_19del |
| 3 | human | 80219 | COQ10B | coenzyme Q10B | NM_001320820.1 | 81.9% | 81.9% | 0_1ins129 |
| 4 | human | 80219 | COQ10B | coenzyme Q10B | NM_001320818.1 | 78.9% | 78.9% | 103_104ins150 |
| 5 | mouse | 67876 | Coq10b | coenzyme Q10B | NM_026424.3 | 67.5% | 68.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agctcggact ggtcatacgg ccttgagaag ggtagtctcg ggatgccgtc 121 cgaagtcggc gacagcggcc ggggcgcagg cgcccgtgcg gaatggcaga tatttagctt 181 cctgtggtat actgatgagc agaactcttc cactacatac ctcaattttg cctaaggaga 241 tatgtgcacg aactttcttc aaaatcactg caccattaat aaacaaaagg aaagaatatt 301 cagagagaag aattttagga tattcaatgc aggaaatgta tgatgtagta tcgggagtgg 361 aggattacaa gcattttgtt ccttggtgca aaaaaTCAGA TGTTATATCA AAGAGATCTG 421 GATATTGTAA AACAAGATTA GAAATTGGAT TTCCACCTGT GTTGGAGCGA TATACATCAG 481 TAGTAACCTT GGTGAAACCT CATTTAGTAA AGGCATCTTG TACTGATGGG AGACTTTTCA 541 ATCATTTGGA GACTATTTGG CGTTTTAGCC CAGGTCTTCC TGGCTACCCA AGAACTTGTA 601 CCTTGGATTT TTCAATTTCT TTTGAATTTC GATCACTTCT ACATTCCCAG CTTGCCACAC 661 TCTTTTTTGA TGAAGTTGTG AAGCAGATGG TAGCTGCCTT TGAAAGAAGA GCATGTAAGC 721 TGTATGGTCC AGAAACAAAT ATACCTCGGG AGTTAATGCT TCATGAAGTC CATCACACAT 781 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 841 GTCTCGATTC TACGTAGTAA TGAACTAGTC GTAACTTGAA AGTATTTCGA TTTCTTGGCT 901 TTATATATCT TGTGGAAAGG ACGAACCCAA CCAGGGGATC CGATGCCTAC GCGTTAAGTC 961 gacaatcaac ctctggatta caaaatttgt gaaagatt