Transcript: Human NM_025147.4

Homo sapiens coenzyme Q10B (COQ10B), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-04
Taxon:
Homo sapiens (human)
Gene:
COQ10B (80219)
Length:
2240
CDS:
139..855

Additional Resources:

NCBI RefSeq record:
NM_025147.4
NBCI Gene record:
COQ10B (80219)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_025147.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430523 CACCTGTGTTGGAGCGATATA pLKO_005 527 CDS 100% 13.200 18.480 N COQ10B n/a
2 TRCN0000136405 CTAAGGAGATATGTGCACGAA pLKO.1 305 CDS 100% 2.640 3.696 N COQ10B n/a
3 TRCN0000424335 GCCGTATGGCTTAGGATATTT pLKO_005 1283 3UTR 100% 15.000 12.000 N COQ10B n/a
4 TRCN0000425920 CAATCATTTGGAGACTATTTG pLKO_005 612 CDS 100% 13.200 9.240 N COQ10B n/a
5 TRCN0000250999 TCAATCATTTGGAGACTATTT pLKO_005 611 CDS 100% 13.200 9.240 N Coq10b n/a
6 TRCN0000135717 GCAGAACTCTTCCACTACATA pLKO.1 272 CDS 100% 5.625 3.938 N COQ10B n/a
7 TRCN0000133671 GCATTTGTTAAACGCAGCTTT pLKO.1 946 3UTR 100% 4.950 3.465 N COQ10B n/a
8 TRCN0000135124 CTCTTCCACTACATACCTCAA pLKO.1 278 CDS 100% 4.050 2.835 N COQ10B n/a
9 TRCN0000133691 GAGCAGAAGTTGAAACTGTAA pLKO.1 1131 3UTR 100% 4.950 2.970 N COQ10B n/a
10 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 1352 3UTR 100% 4.950 2.475 Y ERN2 n/a
11 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 1352 3UTR 100% 4.950 2.475 Y P3H4 n/a
12 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 1352 3UTR 100% 4.950 2.475 Y P3H4 n/a
13 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 1354 3UTR 100% 4.950 2.475 Y CFLAR n/a
14 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 1354 3UTR 100% 4.950 2.475 Y C19orf31 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_025147.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04192 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04192 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478429 ACCCAACCAGGGGATCCGATGCCT pLX_317 53.8% 100% 100% V5 n/a
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