Construct: ORF TRCN0000478439
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006009.1_s317c1
- Derived from:
- ccsbBroadEn_07437
- DNA Barcode:
- ATCCGCCCTTAGCAGCACTGAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBPL1 (9519)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478439
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9519 | TBPL1 | TATA-box binding protein li... | NM_001253676.1 | 99.8% | 99.4% | 131T>N |
| 2 | human | 9519 | TBPL1 | TATA-box binding protein li... | NM_004865.4 | 99.8% | 99.4% | 131T>N |
| 3 | human | 9519 | TBPL1 | TATA-box binding protein li... | XM_017011513.1 | 99.8% | 99.4% | 131T>N |
| 4 | human | 9519 | TBPL1 | TATA-box binding protein li... | XM_024446592.1 | 99.8% | 99.4% | 131T>N |
| 5 | mouse | 237336 | Tbpl1 | TATA box binding protein-li... | NM_011603.5 | 94% | 99.4% | (many diffs) |
| 6 | mouse | 237336 | Tbpl1 | TATA box binding protein-li... | XM_006512730.3 | 94% | 99.4% | (many diffs) |
| 7 | mouse | 237336 | Tbpl1 | TATA box binding protein-li... | XR_001779520.1 | 11.3% | (many diffs) | |
| 8 | mouse | 237336 | Tbpl1 | TATA box binding protein-li... | XR_001779519.1 | 11.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 624
- ORF length:
- 558
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgcagacagt gatgttgcat tggacattct aattacaaat gtagtctgtg 121 tttttagaac aagatgtcat ttaaacttaa ggaagattgc tttggaagga gcaaatgtaa 181 tttataaacg tgatgntgga aaagtattaa tgaagcttag aaaacctaga attacagcta 241 caatttggtc cTCAGGAAAA ATTATTTGCA CTGGAGCAAC AAGTGAAGAA GAAGCTAAAT 301 TTGGTGCCAG ACGCTTAGCC CGTAGTCTGC AGAAACTAGG TTTTCAGGTA ATATTTACAG 361 ATTTTAAGGT TGTTAACGTT CTGGCAGTGT GTAACATGCC ATTTGAAATC CGTTTGCCAG 421 AATTCACAAA GAACAATAGA CCTCATGCCA GTTACGAACC TGAACTTCAT CCTGCTGTGT 481 GCTATCGGAT AAAATCTCTA AGAGCTACAT TACAGATTTT TTCAACAGGA AGTATCACAG 541 TAACAGGGCC CAATGTAAAG GCTGTTGCTA CTGCTGTGGA ACAGATTTAC CCATTTGTGT 601 TTGAAAGCAG GAAAGAAATT TTATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 661 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 721 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TCCGCCCTTA 781 GCAGCACTGA ATCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 841 att