Transcript: Human NM_001253676.1

Homo sapiens TATA-box binding protein like 1 (TBPL1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-07-05
Taxon:
Homo sapiens (human)
Gene:
TBPL1 (9519)
Length:
1635
CDS:
606..1166

Additional Resources:

NCBI RefSeq record:
NM_001253676.1
NBCI Gene record:
TBPL1 (9519)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001253676.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274265 CTGCTGTGTGCTATCGGATAA pLKO_005 1012 CDS 100% 10.800 15.120 N TBPL1 n/a
2 TRCN0000013464 GCCAGTTACGAACCTGAACTT pLKO.1 987 CDS 100% 4.950 6.930 N TBPL1 n/a
3 TRCN0000274262 CCAAACCTTGCTGTAATATAA pLKO_005 1323 3UTR 100% 15.000 10.500 N TBPL1 n/a
4 TRCN0000274327 CAAGTGAAGAAGAAGCTAAAT pLKO_005 820 CDS 100% 13.200 9.240 N TBPL1 n/a
5 TRCN0000119930 CCTAGAATTACAGCTACAATT pLKO.1 765 CDS 100% 13.200 9.240 N Tbpl1 n/a
6 TRCN0000329247 CCTAGAATTACAGCTACAATT pLKO_005 765 CDS 100% 13.200 9.240 N Tbpl1 n/a
7 TRCN0000375314 GGGCACCAAAGAACCTGTAAA pLKO_005 1549 3UTR 100% 13.200 9.240 N Tbpl1 n/a
8 TRCN0000013463 CACCACTTAATTGGTTAGAAT pLKO.1 1169 3UTR 100% 5.625 3.938 N TBPL1 n/a
9 TRCN0000013466 GTGTGTAACATGCCATTTGAA pLKO.1 927 CDS 100% 5.625 3.938 N TBPL1 n/a
10 TRCN0000013467 TGTGGAACAGATTTACCCATT pLKO.1 1115 CDS 100% 4.050 2.835 N TBPL1 n/a
11 TRCN0000274329 TGTGGAACAGATTTACCCATT pLKO_005 1115 CDS 100% 4.050 2.835 N TBPL1 n/a
12 TRCN0000013465 ACCTAGAATTACAGCTACAAT pLKO.1 764 CDS 100% 5.625 3.375 N TBPL1 n/a
13 TRCN0000274264 ACCTAGAATTACAGCTACAAT pLKO_005 764 CDS 100% 5.625 3.375 N TBPL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001253676.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000478439 ATCCGCCCTTAGCAGCACTGAATC pLX_317 66.2% 99.8% 99.4% V5 131T>N n/a
2 ccsbBroadEn_07437 pDONR223 100% 99.6% 98.9% None 131T>N;536T>N n/a
3 ccsbBroad304_07437 pLX_304 0% 99.6% 98.9% V5 131T>N;536T>N n/a
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