Construct: ORF TRCN0000478456
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013858.1_s317c1
- Derived from:
- ccsbBroadEn_05023
- DNA Barcode:
- ACGAACGCAACATTGTAGAGCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNIH3 (149111)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478456
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NM_152495.2 | 100% | 100% | |
| 2 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NM_001322305.2 | 90% | 90% | 149_150ins48 |
| 3 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NM_001322303.2 | 87.3% | 76.8% | (many diffs) |
| 4 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NM_001322304.2 | 82.9% | 78.6% | (many diffs) |
| 5 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NM_001322302.2 | 76.3% | 67.6% | (many diffs) |
| 6 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136288.1 | 24.4% | (many diffs) | |
| 7 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136290.1 | 23.1% | (many diffs) | |
| 8 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136289.1 | 22.4% | (many diffs) | |
| 9 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136297.2 | 22.2% | (many diffs) | |
| 10 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136292.2 | 21.8% | (many diffs) | |
| 11 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136295.2 | 21.4% | (many diffs) | |
| 12 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136296.2 | 21.2% | (many diffs) | |
| 13 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136291.2 | 21.1% | (many diffs) | |
| 14 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136294.2 | 19.9% | (many diffs) | |
| 15 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136293.2 | 19.6% | (many diffs) | |
| 16 | human | 149111 | CNIH3 | cornichon family AMPA recep... | NR_136287.2 | 14.4% | 1_858del;1054_1055ins113;1226_2426del | |
| 17 | mouse | 72978 | Cnih3 | cornichon family AMPA recep... | NM_001160211.1 | 91% | 100% | (many diffs) |
| 18 | mouse | 72978 | Cnih3 | cornichon family AMPA recep... | NM_028408.3 | 81.3% | 89.3% | (many diffs) |
| 19 | mouse | 72978 | Cnih3 | cornichon family AMPA recep... | XM_030243142.1 | 77.8% | 85.5% | (many diffs) |
| 20 | mouse | 72978 | Cnih3 | cornichon family AMPA recep... | NM_001160212.1 | 61.2% | 69.3% | (many diffs) |
| 21 | mouse | 72978 | Cnih3 | cornichon family AMPA recep... | XM_006496989.4 | 54.8% | 62% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cttcactttc gctgcgttct gctacatgct gtctctggtg ctgtgcgctg 121 cgctcatctt cttcgccatc tggcacataa ttgcctttga tgagttaagg acagatttta 181 agagccccat agaccagtgc aatcctgttc atgcgaggga acggttgagg aacatcgagc 241 gcaTCTGCTT CCTTCTGCGA AAGCTGGTGC TGCCAGAATA CTCCATCCAT AGCCTCTTCT 301 GCATTATGTT CCTGTGTGCG CAAGAGTGGC TCACGCTGGG GCTGAATGTC CCTCTACTTT 361 TCTATCACTT CTGGAGGTAT TTCCACTGTC CAGCAGATAG CTCAGAACTA GCCTACGACC 421 CACCGGTGGT CATGAATGCC GACACTTTGA GTTACTGTCA GAAGGAGGCC TGGTGTAAGC 481 TGGCCTTCTA TCTCCTCTCC TTCTTCTACT ACCTTTACTG CATGATCTAC ACTTTAGTGA 541 GCTCTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACGAACGCA ACATTGTAGA GCCGAACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt