Construct: ORF TRCN0000478469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017887.1_s317c1
- Derived from:
- ccsbBroadEn_13744
- DNA Barcode:
- GAAAGTCCGTCCATTGTTCGGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSP1P3 (729862)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478469
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 729862 | LSP1P3 | LSP1 pseudogene 3 | NR_033961.1 | 85.8% | (many diffs) | |
| 2 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001013253.2 | 20.7% | 17.9% | (many diffs) |
| 3 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001013254.1 | 20.7% | 17.9% | (many diffs) |
| 4 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001013255.1 | 20.7% | 17.9% | (many diffs) |
| 5 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001289005.1 | 20.7% | 17.9% | (many diffs) |
| 6 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_002339.3 | 17.5% | 15.1% | (many diffs) |
| 7 | human | 6801 | STRN | striatin | XM_005264519.5 | 13.7% | 10.7% | (many diffs) |
| 8 | human | 4046 | LSP1 | lymphocyte specific protein 1 | NM_001242932.1 | 13.1% | 11.3% | (many diffs) |
| 9 | human | 6801 | STRN | striatin | NM_003162.4 | 13% | 10.2% | (many diffs) |
| 10 | human | 6801 | STRN | striatin | XM_011533073.2 | 12.6% | 9.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 456
- ORF length:
- 387
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccgtggcc agtaccaaga gtcggtggga gacgggtgag gtacaggctc 121 agtctgcggc caagactctg tcctgcaagg atattgtggc tGGAGACATG AGCAACAAAA 181 GCTTCTGGGA GCAGAAGGGA GGCTCCAAGA CCTCATCAAC AATTAAGGCC CAGATTGCCT 241 TCCTACAGGG AGAAGGGAAG GGCCAAGAAA ATTTGAAGAA GGATCTTGTG AGGATGATCA 301 AAATGTTGGA GTATGCTCTT AAACAGAAAA GAGCCAAATA CCACAAGTTG AAATATGGGA 361 CAGAATTGAA TCAGGGAGCT ATGAAGCCTC CAAGCTATGA TTCTGATGAA GGTAATGAAA 421 TAGAAGTGCA GCCACAACAA AACAGCCAGT TAATGTTGCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 AGAAAGTCCG TCCATTGTTC GGGAGACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt