Construct: ORF TRCN0000478484
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009607.1_s317c1
- Derived from:
- ccsbBroadEn_10243
- DNA Barcode:
- GGCGCCTTGTGGTGGTGCCAATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSPC1 (55269)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478484
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55269 | PSPC1 | paraspeckle component 1 | NM_001363660.2 | 100% | 100% | |
| 2 | human | 55269 | PSPC1 | paraspeckle component 1 | XM_011535141.3 | 95.6% | 94.6% | (many diffs) |
| 3 | human | 55269 | PSPC1 | paraspeckle component 1 | XM_011535142.3 | 95.3% | 94.3% | (many diffs) |
| 4 | human | 55269 | PSPC1 | paraspeckle component 1 | NM_001354908.2 | 94.2% | 91% | 1159_1187del;1209_1251del |
| 5 | human | 55269 | PSPC1 | paraspeckle component 1 | XM_011535139.2 | 94.2% | 91% | 1159_1187del;1209_1251del |
| 6 | human | 55269 | PSPC1 | paraspeckle component 1 | XM_011535138.3 | 87.6% | 86% | (many diffs) |
| 7 | human | 55269 | PSPC1 | paraspeckle component 1 | XM_011535137.2 | 83.1% | 82.5% | (many diffs) |
| 8 | human | 55269 | PSPC1 | paraspeckle component 1 | NM_001042414.3 | 74.6% | 73.9% | (many diffs) |
| 9 | human | 55269 | PSPC1 | paraspeckle component 1 | NM_001354909.2 | 74.6% | 73.9% | (many diffs) |
| 10 | human | 55269 | PSPC1 | paraspeckle component 1 | NR_003272.4 | 70.2% | 1_188del;1368_1679del | |
| 11 | human | 55269 | PSPC1 | paraspeckle component 1 | NR_156729.2 | 69.4% | 1_188del;1347_1365del;1387_1698del | |
| 12 | human | 55269 | PSPC1 | paraspeckle component 1 | XR_941617.2 | 61.4% | (many diffs) | |
| 13 | human | 55269 | PSPC1 | paraspeckle component 1 | NR_044998.2 | 48% | 1_279del;1459_2455del | |
| 14 | human | 55269 | PSPC1 | paraspeckle component 1 | NR_149053.1 | 47.6% | 1_279del;1438_1456del;1478_2474del | |
| 15 | human | 55269 | PSPC1 | paraspeckle component 1 | NR_149052.1 | 43.6% | (many diffs) | |
| 16 | mouse | 66645 | Pspc1 | paraspeckle protein 1 | NM_025682.3 | 68% | 72.3% | (many diffs) |
| 17 | mouse | 66645 | Pspc1 | paraspeckle protein 1 | XM_006519393.2 | 27.7% | 29.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1245
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gttaagagga aacctgaagc aagtgcgcat tgagaaaaac ccggcccgcc 121 ttcgcgccct ggagtccgcg gtgggcgaga gcgagccggc ggccgcggca gccatggcgc 181 tcgctcttgc cggggagccg gcaccgcccg cgcccgcgcc tccagaggac cacccggacg 241 aggagatggg gttcactatc gacatcaaga gtttcctcaa gccgggcgag aagacgtaca 301 cgcagcgctg ccgcctcttc gtgggaaatc tgcccaccga catcacggag gaggacttca 361 agaggctctt cgaacgctat ggcgagccca gcgaagtctt catcaaccgg gaccgtggct 421 tcggcttcat ccgcttggaa tccagaaccc tggctgaaat tgcaaaagca gagctggacg 481 gcaccattct caagagcaga cctctacgga ttcgcttcgc tacacatgga gcagccttga 541 ctgtcaagaa cctttctcca gttgtttcca atgagctgct agagcaagca ttttctcagt 601 ttggtccagt agagaaagct gttgtggttg tggatgatcg cggtagagct acaggaaaag 661 gttttgtaga gtttgcagca aaacctcctg cacgaaaggc tctggaaaga tgtggtgatg 721 gggcattctt gctaacaacg acccctcgtc cagtcattgt ggaacccatg gagcagtttg 781 atgatgaaga tggcttgcca gagaagctga tgcagaaaac tcaacaatat cataaggaaa 841 gagaacaacc accacgtttt gctcaacctg ggacatttga atttgagtat gcatctcgat 901 GGAAGGCTCT TGATGAAATG GAAAAGCAGC AGCGTGAGCA GGTTGATAGA AACATCAGAG 961 AAGCCAAAGA GAAACTGGAG GCAGAAATGG AAGCAGCTAG GCATGAACAC CAATTAATGC 1021 TAATGAGGCA AGATCTAATG AGGCGTCAAG AAGAACTCAG ACGCTTGGAA GAACTCAGAA 1081 ACCAAGAGTT GCAAAAACGG AAGCAAATAC AACTAAGACA TGAAGAGGAG CATCGGCGGC 1141 GTGAGGAAGA AATGATCCGA CACAGAGAAC AGGAGGAACT GAGGCGACAG CAAGAGGGCT 1201 TTAAGCCAAA CTACATGGAA AATGGTGATA AAAGAAAATG TGGCTGCCCA ACTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA GGCGCCTTGT GGTGGTGCCA ATAAACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt