Construct: ORF TRCN0000478554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009667.1_s317c1
- Derived from:
- ccsbBroadEn_10870
- DNA Barcode:
- CCCATGGCGTGAGTCTAGATGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HCFC1 (3054)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3054 | HCFC1 | host cell factor C1 | XM_017029471.2 | 21.2% | 21.2% | 1283C>T;1285_6039del |
| 2 | human | 3054 | HCFC1 | host cell factor C1 | NM_005334.3 | 21% | 20.9% | 1283C>T;1285_6105del |
| 3 | human | 3054 | HCFC1 | host cell factor C1 | XM_011531148.3 | 20.9% | 20.9% | 1283C>T;1285_6114del |
| 4 | human | 3054 | HCFC1 | host cell factor C1 | XM_011531147.3 | 20.9% | 20.9% | 1283C>T;1285_6117del |
| 5 | human | 3054 | HCFC1 | host cell factor C1 | XM_006724816.3 | 20.5% | 20.5% | 1283C>T;1285_6237del |
| 6 | human | 3054 | HCFC1 | host cell factor C1 | XM_006724815.3 | 20.5% | 20.5% | 1283C>T;1285_6240del |
| 7 | mouse | 15161 | Hcfc1 | host cell factor C1 | NM_008224.4 | 18.8% | 20.8% | (many diffs) |
| 8 | mouse | 15161 | Hcfc1 | host cell factor C1 | XM_017318387.1 | 18.8% | 20.8% | (many diffs) |
| 9 | mouse | 15161 | Hcfc1 | host cell factor C1 | XM_006527836.1 | 18.4% | 20.3% | (many diffs) |
| 10 | mouse | 15161 | Hcfc1 | host cell factor C1 | XM_006527835.1 | 18.4% | 20.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1350
- ORF length:
- 1284
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcggccgtg tcgcccgcca acttgccagc ggtgcttctg cagccccgct 121 ggaagcgagt ggtgggctgg tcgggtccgg tgccacggcc ccgccacggc caccgcgccg 181 tggccatcaa ggagctcatc gtggtgtttg gcggcggcaa cgagggaata gtggacgaac 241 tgcacgtgta caacacggca accaaccagt ggttcatccc agccgtgagg ggggacattc 301 cccctgggtg tgcagcctat ggcttcgtgt gtgacgggac tcgcctcctg gtgtttggtg 361 ggatggtgga gtatgggaaa tacagcaatg acctctacga actccaggcg agccggtggg 421 agtggaagag actcaaagca aagacgccca aaaacgggcc ccctccgtgt cctcgactcg 481 ggcacagctt ctcccttgtg ggcaacaaat gctacctgtt tgggggtctg gccaatgata 541 gcgaggaccc aaagaacaac attccaaggt acctgaatga cttatatatc ctggaattac 601 ggccaggctc tggagtggta gcctgggaca ttcccatcac ttacggggtc ctaccaccac 661 cccgggagtc acatactgcc gtggtctaca ccgaaaaaga caataagaag tccaagctgg 721 tgatctacgg cgggatgagt ggctgcaggc tgggggacct gtggacccta gatattgaca 781 ccctgacgtg gaataagccc agtctcagcg gggtggcgcc tcttcctcgc agtctccact 841 cggcaaccac catcggaaat aaaatgtacg tgtttggtgg ctgggtgcct ctcgtcatgg 901 atgacgtcaa agtggccaca cacgagaagg agtggaagtg taccaacacg ctggcttgtc 961 tcaacctgga taccatggcc tgggagacca tcctgatgga tacactggag gacaacatcc 1021 cccgtgctcg ggctggcCAC TGCGCAGTCG CCATCAACAC CCGCCTGTAC ATTTGGAGTG 1081 GGCGTGACGG CTACCGCAAG GCCTGGAACA ACCAGGTCTG CTGCAAGGAC CTCTGGTACC 1141 TAGAGACAGA AAAGCCACCA CCCCCAGCCC GAGTACAACT GGTACGCGCC AACACCAACT 1201 CCCTGGAGGT GAGCTGGGGG GCAGTGGCAA CAGCCGACAG CTACCTTCTC CAGCTCCAGA 1261 AATATGACAT TCCTGCCACG GCTGCTACTG CCACCTCCCC TACACCCAAT CCGGTCCCAT 1321 CTGTGCCTGC CAACCCTCCC AAGAGCCTTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1381 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1441 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCCAT 1501 GGCGTGAGTC TAGATGATTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1561 tgaaagatt