Construct: ORF TRCN0000478565

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009546.1_s317c1
Derived from:
ccsbBroadEn_06508
DNA Barcode:
GGTCTGGTAAACGCTAGTGTGTCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KRT18 (3875)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478565
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3875 KRT18 keratin 18 NM_000224.3 99.9% 99.7% 502T>C
2 human 3875 KRT18 keratin 18 NM_199187.1 99.9% 99.7% 502T>C
3 human 284085 KRT18P55 keratin 18 pseudogene 55 NR_028334.1 59.6% (many diffs)
4 human 693207 MIR622 microRNA 622 NR_030754.1 7% (many diffs)
5 mouse 16668 Krt18 keratin 18 NM_010664.2 83% 86.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1356
ORF length:
1290
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag cttcaccact cgctccacct tctccaccaa ctaccggtcc ctgggctctg
121tccaggcgcc cagctacggc gcccggccgg tcagcagcgc ggccagcgtc tatgcaggcg
181ctgggggctc tggttcccgg atctccgtgt cccgctccac cagcttcagg ggcggcatgg
241ggtccggggg cctggccacc gggatagccg ggggtctggc aggaatggga ggcatccaga
301acgagaagga gaccatgcaa agcctgaacg accgcctggc ctcttacctg gacagagtga
361ggagcctgga gaccgagaac cggaggctgg agagcaaaat ccgggagcac ttggagaaga
421agggacccca ggtcagagac tggagccatt acttcaagat catcgaggac ctgagggctc
481agatcttcgc aaatactgtg gacaatgccc gcatcgttct gcagattgac aatgcccgtc
541ttgctgctga tgactttaga gtcaagcatg agacagagct ggccatgcgc cagtctgtgg
601agaacgacat ccatgggctc cgcaaggtca ttgatgacac caatatcaca cgactgcagc
661tggagacaga gatcgaggct ctcaaggagg agctgctctt catgaagaag aaccacgaag
721aggaagtaaa aggcctacaa gcccagattg ccagctctgg gttgaccgtg gaggtagatg
781cccccaaatc tcaggacctc gccaagatca tggcagacat ccgggcccaa tatgacgagc
841tggctcggaa gaaccgagag gagctagaca agtactggtc tcagcagatt gaggagagca
901ccacagtggt caccacacag tctgctgagg ttggagctgc tgagacgacg ctcacagagc
961tgagacgtac agtccagtcc ttggagatcg acctggactc catgagaaat ctgaaggcca
1021gcttggagaa cagcctgagg gaggtggagg cccgctacgc cctacagatG GAGCAGCTCA
1081ACGGGATCCT GCTGCACCTT GAGTCAGAGC TGGCACAGAC CCGGGCAGAG GGACAGCGCC
1141AGGCCCAGGA GTATGAGGCC CTGCTGAACA TCAAGGTCAA GCTGGAGGCT GAGATCGCCA
1201CCTACCGCCG CCTGCTGGAA GATGGCGAGG ACTTTAATCT TGGTGATGCC TTGGACAGCA
1261GCAACTCCAT GCAAACCATC CAAAAGACCA CCACCCGCCG GATAGTGGAT GGCAAAGTGG
1321TGTCTGAGAC CAATGACACC AAAGTTCTGA GGCATTACCC AACTTTCTTG TACAAAGTGG
1381TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1441CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1501AGGTCTGGTA AACGCTAGTG TGTCCACGCG TTAAGTCgac aatcaacctc tggattacaa
1561aatttgtgaa agatt

Download FASTA (ORF) (Full)