Construct: ORF TRCN0000478570
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018184.1_s317c1
- Derived from:
- ccsbBroadEn_12542
- DNA Barcode:
- CGAAACTACGGCTGTCCGCAGGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A23 (79085)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478570
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528284.2 | 95% | 91.9% | (many diffs) |
| 2 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528283.2 | 94.5% | 93.8% | (many diffs) |
| 3 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528281.2 | 91.8% | 85% | (many diffs) |
| 4 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528282.2 | 91.5% | 92% | (many diffs) |
| 5 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528280.2 | 87.3% | 86.6% | (many diffs) |
| 6 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528278.2 | 86.2% | 79.9% | (many diffs) |
| 7 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528277.2 | 84.5% | 78.4% | (many diffs) |
| 8 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_017027285.2 | 84.4% | 78.1% | (many diffs) |
| 9 | human | 79085 | SLC25A23 | solute carrier family 25 me... | NM_024103.3 | 82.8% | 76.2% | (many diffs) |
| 10 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528285.2 | 81% | 74.5% | (many diffs) |
| 11 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_017027288.2 | 78.6% | 77.7% | (many diffs) |
| 12 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528274.2 | 77.1% | 70.6% | 582A>G;1362_1542del;1628_1872del |
| 13 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_017027289.2 | 74% | 67.8% | (many diffs) |
| 14 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_017027292.2 | 73.9% | 71.1% | (many diffs) |
| 15 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_017027290.2 | 72% | 65.7% | (many diffs) |
| 16 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528275.2 | 69.7% | 63.3% | 482_483ins141;1221_1401del;1487_1731del |
| 17 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528276.2 | 68.2% | 61.9% | (many diffs) |
| 18 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_017027291.2 | 65% | 59% | (many diffs) |
| 19 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_011528279.2 | 62.4% | 56.3% | (many diffs) |
| 20 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_017027286.2 | 60.7% | 54.6% | (many diffs) |
| 21 | human | 79085 | SLC25A23 | solute carrier family 25 me... | XM_017027287.2 | 54.9% | 49% | (many diffs) |
| 22 | mouse | 66972 | Slc25a23 | solute carrier family 25 (m... | NM_025877.4 | 71% | 73.4% | (many diffs) |
| 23 | mouse | 66972 | Slc25a23 | solute carrier family 25 (m... | XM_011246575.2 | 69.3% | 71.6% | (many diffs) |
| 24 | mouse | 66972 | Slc25a23 | solute carrier family 25 (m... | XM_011246576.2 | 61.7% | 63.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1512
- ORF length:
- 1446
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttagcatgcg ggggagcccg ggcgacgcgg agcggcggca gcgctggggt cgcctgttcg 121 aggagctgga cagtaacaag gatggccgcg tggacgtgca cgagttgcgc caggggctgg 181 ccaggctggg cgggggcaac ccagaccccg gcgcccaaca gggtatctcc tctgagggtg 241 atgctgaccc agatggcggg ctcgacctgg aggaattttc ccgctatctg caggagcggg 301 aacagcgtct gctgctcatg tttcacagtc ttgaccggaa ccaggatggt cacattgatg 361 tctctgagat ccaacagagt ttccgagctc tgggcatttc catctcgctg gagcaggctg 421 agaaaatttt gcacagcatg gaccgagacg gcacaatgac cattgactgg caagaatggc 481 gcgaccactt cctgttgcat tcgctggaaa atgtggagga cgtgctgtat ttctggaagc 541 attccacgct ctcttctgct gggttctctg cctggataaa agattctacg gctgaacaga 601 atcgttcaaa aaccacggtc ttggccaggc gcagtggctc acacctgaaa tcccagcact 661 ttgggaggcc gaagtgggcg gatcacgagg tcctggacat tggcgagtgc ctgacagtgc 721 cggacgagtt ctcaaagcaa gagaagctga cgggcatgtg gtggaaacag ctggtggccg 781 gcgcagtggc aggtgccgtg tcacggacag gcacggcccc tctggaccgc ctcaaggtct 841 tcatgcaggt ccatgcctca aagaccaacc ggctgaacat ccttgggggg cttcgaagca 901 tggtccttga gggaggcatc cgctccctgt ggcgcggcaa tggtattaat gtactcaaga 961 ttgcccccga gtcagctatc aagttcatgg cctatgaaca gatcaagagg gccatcctgg 1021 ggcagcagga gacactgcat gtgcaggagc gcttcgtggc tggctccctg gctggtgcca 1081 cagcccaaac catcatttac cctatggagg tgctgaagac gcggctgacc ttgcgccgga 1141 cgggccagta taaggggctg ctggactgcg ccaggcgtat cctggagagg gaggggcccc 1201 gtgccttcta ccgcggctac ctccccaacg tgctgggcat caTCCCCTAT GCGGGCATCG 1261 ACCTGGCCGT CTACGAGACT CTGAAGAACT GGTGGCTTCA GCAGTACAGC CACGACTCGG 1321 CAGACCCAGG CATCCTCGTG CTCCTGGCCT GCGGTACCAT ATCCAGCACC TGCGGCCAGA 1381 TAGCCAGTTA CCCGCTGGCC CTGGTCCGGA CCCGCATGCA GGCACAAGGC TGGAGTACAG 1441 TGGCTCGATT TCAGATCACT GCAACCTCTG CCTTCCAGGT TCAAGCGATT CTCCTGCCTC 1501 AGCCTCCAGA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1561 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1621 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGA AACTACGGCT GTCCGCAGGT 1681 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t