Construct: ORF TRCN0000478594
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018234.1_s317c1
- Derived from:
- ccsbBroadEn_00560
- DNA Barcode:
- TCCGATACATCGCTTTAATTTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF14 (2259)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478594
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_175929.2 | 100% | 100% | |
| 2 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321939.1 | 85.3% | 84.9% | 208_209ins111 |
| 3 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321945.2 | 84.5% | 84.5% | 0_1ins117 |
| 4 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321948.2 | 84.5% | 84.5% | 0_1ins117 |
| 5 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321947.2 | 79.3% | 79.3% | 0_1ins156 |
| 6 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_004115.3 | 76.8% | 71.2% | (many diffs) |
| 7 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321933.1 | 74.2% | 72.6% | (many diffs) |
| 8 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321938.2 | 74.2% | 72.6% | (many diffs) |
| 9 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321940.1 | 74.2% | 72.6% | (many diffs) |
| 10 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321941.1 | 72.8% | 73% | 0_1ins203;2_3delTG;6A>T |
| 11 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321932.1 | 72.6% | 72.2% | 1_1delAins206;4delC |
| 12 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321936.1 | 72.6% | 72.2% | 1_1delAins206;4delC |
| 13 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321944.1 | 72.6% | 72.2% | 1_1delAins206;4delC |
| 14 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321931.1 | 64.6% | 64.6% | 0_1ins267 |
| 15 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321934.1 | 64.6% | 64.6% | 0_1ins267 |
| 16 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321935.1 | 64.6% | 64.6% | 0_1ins267 |
| 17 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321942.1 | 64.6% | 64.6% | 0_1ins267 |
| 18 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321943.1 | 64.6% | 64.6% | 0_1ins267 |
| 19 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321946.2 | 64.6% | 64.6% | 0_1ins267 |
| 20 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321949.1 | 64.6% | 64.6% | 0_1ins267 |
| 21 | human | 2259 | FGF14 | fibroblast growth factor 14 | NM_001321937.1 | 60% | 57.5% | (many diffs) |
| 22 | mouse | 14169 | Fgf14 | fibroblast growth factor 14 | NM_207667.3 | 95.5% | 98% | (many diffs) |
| 23 | mouse | 14169 | Fgf14 | fibroblast growth factor 14 | XM_017315845.2 | 75.3% | 78.1% | (many diffs) |
| 24 | mouse | 14169 | Fgf14 | fibroblast growth factor 14 | NM_010201.4 | 71.5% | 70.1% | (many diffs) |
| 25 | mouse | 14169 | Fgf14 | fibroblast growth factor 14 | XM_011244952.3 | 60.9% | 63.4% | (many diffs) |
| 26 | mouse | 14169 | Fgf14 | fibroblast growth factor 14 | XM_006518549.4 | 59.1% | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 825
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtaaaaccg gtgcccctct tcaggagaac tgatttcaaa ttattattat 121 gcaaccacaa ggatctcttc tttctcaggg tgtctaagct gctggattgc ttttcgccca 181 aatcaatgtg gtttctttgg aacattttca gcaaaggaac gcatatgctg cagtgtcttt 241 gtggcaagag tcttaagaaa aacaagaacc caactgatcc ccagctcaag ggtatagtga 301 ccaggttata ttgcaggcaa ggctactact tgcaaatgca ccccgatgga gctctcgatg 361 gaaccaagga tgacagcact aattctacac tcttcaacct cataccagtg ggactacgtg 421 ttgttgccat ccagggagtg aaaacagggt tgtatatagc catGAATGGA GAAGGTTACC 481 TCTACCCATC AGAACTTTTT ACCCCTGAAT GCAAGTTTAA AGAATCTGTT TTTGAAAATT 541 ATTATGTAAT CTACTCATCC ATGTTGTACA GACAACAGGA ATCTGGTAGA GCCTGGTTTT 601 TGGGATTAAA TAAGGAAGGG CAAGCTATGA AAGGGAACAG AGTAAAGAAA ACCAAACCAG 661 CAGCTCATTT TCTACCCAAG CCATTGGAAG TTGCCATGTA CCGAGAACCA TCTTTGCATG 721 ATGTTGGGGA AACGGTCCCG AAGCCTGGGG TGACGCCAAG TAAAAGCACA AGTGCGTCTG 781 CAATAATGAA TGGAGGCAAA CCAGTCAACA AGAGTAAGAC AACATTGCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA TCCGATACAT CGCTTTAATT TGACACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt