Construct: ORF TRCN0000478612
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003435.1_s317c1
- Derived from:
- ccsbBroadEn_15773
- DNA Barcode:
- TTCTAGCTCAGTTGTAAAGACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NIPBL (25836)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478612
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25836 | NIPBL | NIPBL cohesin loading factor | XM_017009331.1 | 7.5% | 7.3% | (many diffs) |
| 2 | human | 25836 | NIPBL | NIPBL cohesin loading factor | XM_017009330.2 | 7.5% | 7.3% | (many diffs) |
| 3 | human | 25836 | NIPBL | NIPBL cohesin loading factor | XM_005248282.5 | 6.6% | 6.4% | (many diffs) |
| 4 | human | 25836 | NIPBL | NIPBL cohesin loading factor | XM_017009329.1 | 6.5% | 6.6% | 1_7419del;7944_7947delTCATinsG |
| 5 | human | 25836 | NIPBL | NIPBL cohesin loading factor | NM_015384.5 | 6.4% | 6.4% | 1_7566del |
| 6 | human | 25836 | NIPBL | NIPBL cohesin loading factor | XM_005248280.3 | 6.4% | 6.4% | 1_7566del;8091_8094delTCATinsG |
| 7 | human | 25836 | NIPBL | NIPBL cohesin loading factor | XM_006714468.2 | 6.2% | 6% | (many diffs) |
| 8 | human | 25836 | NIPBL | NIPBL cohesin loading factor | XM_006714467.2 | 6.2% | 6% | (many diffs) |
| 9 | human | 25836 | NIPBL | NIPBL cohesin loading factor | NM_133433.4 | 6.1% | 5.9% | (many diffs) |
| 10 | mouse | 71175 | Nipbl | Nipped-B homolog (Drosophila) | NM_201232.2 | 5.8% | 6.3% | (many diffs) |
| 11 | mouse | 71175 | Nipbl | Nipped-B homolog (Drosophila) | XM_006519996.3 | 5.8% | 6.3% | (many diffs) |
| 12 | mouse | 71175 | Nipbl | Nipped-B homolog (Drosophila) | XM_017316746.1 | 5.5% | 5.7% | (many diffs) |
| 13 | mouse | 71175 | Nipbl | Nipped-B homolog (Drosophila) | NM_027707.3 | 5.5% | 5.7% | (many diffs) |
| 14 | mouse | 71175 | Nipbl | Nipped-B homolog (Drosophila) | XM_006519994.3 | 5.5% | 5.7% | (many diffs) |
| 15 | mouse | 71175 | Nipbl | Nipped-B homolog (Drosophila) | XM_006519995.2 | 5.5% | 5.7% | (many diffs) |
| 16 | mouse | 71175 | Nipbl | Nipped-B homolog (Drosophila) | XM_017316745.1 | 5.5% | 5.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa atgtttgcca gaaaattcag ctcctttaat cgaatttgca aatgtgtccc 121 agggtatttt attacttctc atgttaaaac aacatttgaa gaatctttgt ggattttctg 181 atagtaaaat tcagaagtac tctccatctg aatctgcaaa agtatatgat aaagcgataa 241 aCCGAAAAAC AGGAGTTCAT TTTCATCCAA AACAAACACT GGACTTCCTG CGGAGTGACA 301 TGGCTAATTC CAAAATCACA GAAGAGGTGA AAAGGAGTAT AGTAAAACAG TATCTAGATT 361 TCAAACTTCT CATGGAACAT CTGGACCCTG ATGAAGAAGA AGAAGAAGGG GAGGTTTCAG 421 CTAGCACAAA TGCTCGGAAC AAAGCAATTA CCTCACTGCT TGGAGGAGGC AGCCCTAAAA 481 ATAATACAGC AGCAGAGACA GAAGATGATG AAAGTGATGG GGAGGATAGA GGAGGAGGCA 541 CTTCAGGGGT GAGGCGGAGG AGGAGTCAAC GTATTTCGCA GCGTATTACG TACCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGATTCT AGCTCAGTTG TAAAGACCCC ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt