Construct: ORF TRCN0000478640
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001369.1_s317c1
- Derived from:
- ccsbBroadEn_07038
- DNA Barcode:
- TACATCAATAACCCCTTTTATCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF354A (6940)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478640
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6940 | ZNF354A | zinc finger protein 354A | NM_005649.3 | 99.8% | 99.8% | 768T>C;897A>C |
| 2 | human | 6940 | ZNF354A | zinc finger protein 354A | XM_017009791.1 | 99.8% | 99.8% | 768T>C;897A>C |
| 3 | human | 6940 | ZNF354A | zinc finger protein 354A | NM_001324339.2 | 92.9% | 92.8% | 0_1ins126;642T>C;771A>C |
| 4 | human | 6940 | ZNF354A | zinc finger protein 354A | XM_011534645.2 | 92.9% | 92.8% | 0_1ins126;642T>C;771A>C |
| 5 | human | 117608 | ZNF354B | zinc finger protein 354B | NM_058230.3 | 88.8% | 84.8% | (many diffs) |
| 6 | mouse | 21408 | Zfp354a | zinc finger protein 354A | NM_009329.4 | 81.4% | 82.6% | (many diffs) |
| 7 | mouse | 21408 | Zfp354a | zinc finger protein 354A | XM_006532790.1 | 81.4% | 82.6% | (many diffs) |
| 8 | mouse | 21408 | Zfp354a | zinc finger protein 354A | NM_001347495.1 | 81.2% | 82.5% | (many diffs) |
| 9 | mouse | 21408 | Zfp354a | zinc finger protein 354A | XM_006532789.1 | 81.2% | 82.5% | (many diffs) |
| 10 | mouse | 21408 | Zfp354a | zinc finger protein 354A | NM_001326566.1 | 80.9% | 81.8% | (many diffs) |
| 11 | mouse | 21408 | Zfp354a | zinc finger protein 354A | XM_011248885.1 | 80.8% | 81.7% | (many diffs) |
| 12 | mouse | 21408 | Zfp354a | zinc finger protein 354A | XM_011248886.1 | 80.8% | 81.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1881
- ORF length:
- 1815
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgctgggcag cgggaagcga ggccccaggt gtcactgacg tttgaggatg 121 tggctgtgct gtttacccga gatgagtgga gaaagctggc cccttctcag agaaacttgt 181 accgggatgt gatgctggag aactatagga acctggtctc actggggctc ccatttacca 241 aaccaaaagt gatctccctg ttgcagcaag gagaagatcc ctgggaggtg gagaaagacg 301 gttctggcgt ctcctctcta ggatcgaaga gcagtcataa aaccacaaag tcaacgcaaa 361 cacaagactc ttcatttcag ggactgatac tgaaaagatc caacaggaat gtaccttggg 421 atttgaaatt agaaaagcct tacatatatg aaggcagatt agagaaaaag caggataaaa 481 agggaagttt tcagatagtt tcagccaccc acaaaaaaat ccccactata gaaagaagcc 541 ataaaaatac tgaattgagc caaaacttca gcccaaagtc agtgcttatt aggcaacaga 601 tacttcccag agaaaaaaca ccaccaaaat gtgaaataca aggaaacagc ctcaaacaga 661 attcacaatt acttaatcaa ccaaaaatta cagcagataa acgctataaa tgtagtctgt 721 gtgaaaaaac cttcattaac acttcatccc ttcgtaaaca tgagaaaaac catagtggag 781 agaaactatt taagtgtaaa gaatgttcaa aagcctttag ccaaagttca gcccttattc 841 aacatcaaat aacgcatact ggagagaaac cctacatatg taaagaatgt gggaaagcct 901 ttactctcag tacatccctt tataaacatc taagaaccca tactgtggag aaatcctaca 961 gctgtaaaga atgtggtaaa tccttcagcc gaaggtcagg cctttttata catcaaaaaa 1021 ttcatgctga agaaaaccct tgtaagtata atccgggtag gaaggcatct agttgcagca 1081 catccctttc tggatgtcaa agaattcatt ctagaaagaa gtcctactta tgtaatgaat 1141 gtggcaacac ctttaagtct agctcatccc ttcgttatca tcagagaatt cacactggag 1201 agaagccttt taaatgtagt gaatgtggga gagccttcag ccagagtgcc tctcttattc 1261 aacatgaaag aattcacacc ggagaaaagc cctatagatg caatgaatgt gggaaaggct 1321 ttacttctat ttcacgactt aatagacacc gaatcattca tactggagag aagttttata 1381 attgtaatga atgtggtaaa gccttaagct cccactcaac acttattatt cacgagcgaa 1441 ttcatactgg agaaaaacca tgtaaatgta aagtatgtgg aaaagccttc agacagagtt 1501 cagctctcat tcaacatcag agaatgcata ctggagaaag accctataaa tgtaacgagt 1561 gtgggaaaac attcaggtgt aactcatcac ttagtaaTCA CCAGAGAATT CATACTGGAG 1621 AGAAACCATA TCGATGTGAG GAATGTGGGA TATCTTTTGG CCAAAGTTCA GCTCTTATTC 1681 AGCATCGAAG GATTCATACA GGAGAAAAAC CCTTTAAATG TAATACATGT GGAAAAACTT 1741 TTAGACAAAG CTCATCACGT ATTGCACATC AGAGAATTCA TACTGGAGAG AAACCCTATG 1801 AATGTAATAC ATGTGGGAAA CTTTTCAACC ATAGGTCATC CCTTACTAAT CATTATAAAA 1861 TTCATATCGA AGAGGACCCC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1921 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1981 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACA TCAATAACCC 2041 CTTTTATCCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt