Construct: ORF TRCN0000478654
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016744.1_s317c1
- Derived from:
- ccsbBroadEn_00634
- DNA Barcode:
- CGGCTAGGGTAGCTGAACACGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GH2 (2689)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478654
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2689 | GH2 | growth hormone 2 | NM_002059.5 | 100% | 100% | |
| 2 | human | 2688 | GH1 | growth hormone 1 | NM_000515.5 | 94.6% | 93% | (many diffs) |
| 3 | human | 2689 | GH2 | growth hormone 2 | NM_022556.4 | 93% | 93% | 171_172ins45 |
| 4 | human | 1442 | CSH1 | chorionic somatomammotropin... | NM_001317.6 | 90.6% | 79.7% | (many diffs) |
| 5 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_020991.3 | 90.1% | 80.1% | (many diffs) |
| 6 | human | 2688 | GH1 | growth hormone 1 | NM_022559.4 | 88.1% | 86.6% | (many diffs) |
| 7 | human | 2689 | GH2 | growth hormone 2 | NM_022558.4 | 87.5% | 60.6% | 452_453insGTGG;648_735del |
| 8 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022579.3 | 87.1% | 74.7% | (many diffs) |
| 9 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022581.3 | 81.4% | 68.2% | (many diffs) |
| 10 | human | 2688 | GH1 | growth hormone 1 | NM_022560.4 | 77.8% | 76% | (many diffs) |
| 11 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321069.2 | 76.4% | 66.8% | (many diffs) |
| 12 | human | 2689 | GH2 | growth hormone 2 | NM_022557.4 | 72.5% | 53.8% | (many diffs) |
| 13 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321068.2 | 66.9% | 58% | (many diffs) |
| 14 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001321067.2 | 66.8% | 56.6% | (many diffs) |
| 15 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_022644.3 | 66.3% | 54.5% | (many diffs) |
| 16 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_022580.3 | 57.4% | 48.3% | (many diffs) |
| 17 | human | 1444 | CSHL1 | chorionic somatomammotropin... | NM_001318.4 | 53.3% | 45.6% | (many diffs) |
| 18 | human | 1443 | CSH2 | chorionic somatomammotropin... | NM_022645.2 | 51.6% | 47% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcaggctcc cggacgtccc tgctcctggc ttttggcctg ctctgcctgt 121 cctggcttca agagggcagt gccttcccaa ccattccctt atccaggctt tttgacaacg 181 ctatgctccg cgcccgtcgc ctgtaccagc tggcatatga cacctatcag gagtttgaag 241 aagcctatat cctgaaggag cagaagtatt cattcctgca gaacccccag acctccctct 301 gcttctcaga gtctattcca acaccttcca acagggtgaa aacgcagcag aaatctaacc 361 tagagctgct ccgcatctcc ctgctgctca tccagtcatg gctggagccc gtgcagctcc 421 TCAGGAGCGT CTTCGCCAAC AGCCTGGTGT ATGGCGCCTC GGACAGCAAC GTCTATCGCC 481 ACCTGAAGGA CCTAGAGGAA GGCATCCAAA CGCTGATGTG GAGGCTGGAA GATGGCAGCC 541 CCCGGACTGG GCAGATCTTC AATCAGTCCT ACAGCAAGTT TGACACAAAA TCGCACAACG 601 ATGACGCACT GCTCAAGAAC TACGGGCTGC TCTACTGCTT CAGGAAGGAC ATGGACAAGG 661 TCGAGACATT CCTGCGCATC GTGCAGTGCC GCTCTGTGGA GGGCAGCTGT GGCTTCTACC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GACGGCTAGG GTAGCTGAAC ACGAGTACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt