Construct: ORF TRCN0000478656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008518.2_s317c1
- Derived from:
- ccsbBroadEn_06855
- DNA Barcode:
- TTCCGTTTGGCCTCCTGAACCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RDX (5962)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5962 | RDX | radixin | NM_002906.3 | 99.8% | 99.8% | 835C>A;982A>G |
| 2 | human | 5962 | RDX | radixin | NM_001260492.2 | 96.4% | 96.3% | 835C>A;982A>G;1750_1812del |
| 3 | human | 5962 | RDX | radixin | NM_001260493.1 | 96.4% | 96.3% | 835C>A;982A>G;1750_1812del |
| 4 | human | 5962 | RDX | radixin | NM_001260494.1 | 76.5% | 74% | (many diffs) |
| 5 | human | 5962 | RDX | radixin | NM_001260495.1 | 39% | 39% | 0_1ins1041;709_771del |
| 6 | human | 5962 | RDX | radixin | NM_001260496.1 | 32% | 29.6% | (many diffs) |
| 7 | mouse | 19684 | Rdx | radixin | NM_001104616.1 | 90% | 98.2% | (many diffs) |
| 8 | mouse | 19684 | Rdx | radixin | NM_009041.3 | 90% | 98.2% | (many diffs) |
| 9 | mouse | 19684 | Rdx | radixin | NM_001104617.1 | 60.2% | 62.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1815
- ORF length:
- 1749
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gaaaccaatc aacgtaagag taactacaat ggatgctgag ctggaatttg 121 ccattcagcc caatacaact ggcaaacaac tttttgacca ggtggtgaaa acagttggtt 181 tgcgtgaggt ctggtttttt gggctgcagt atgtagacag caaaggttat tctacatggc 241 ttaaactaaa taaaaaggta acacagcagg atgttaaaaa agagaatcct ttacagttca 301 agtttagagc taaattcttt cctgaagatg tttctgagga attaattcaa gaaataaccc 361 agagactctt cttcttgcaa gttaaagaag ccatcttaaa tgatgagata tattgcccgc 421 cagaaactgc agttcttttg gcttcctatg ctgtccaagc caagtatgga gattacaata 481 aagagattca taagccaggc tacctggcta atgatagact cctaccccag cgtgtattgg 541 aacaacacaa actaacaaaa gaacagtggg aagaaagaat acagaactgg catgaagaac 601 atagaggaat gttaagggag gattctatga tggaatacct gaagattgca caagatctag 661 aaatgtatgg agtcaactat tttgaaataa aaaataaaaa aggaactgaa ttgtggctag 721 gtgttgatgc tttgggtctg aatatttatg agcatgacga caagttaaca cctaaaattg 781 gttttccctg gagtgaaatc agaaatattt catttaatga caaaaaattt gttataaagc 841 caatcgacaa aaaggcacct gattttgtgt tttatgcacc tcgtctgaga atcaataaga 901 ggattttggc cttatgtatg ggaaaccatg aactatacat gcgaagaagg aagcctgata 961 ctattgaagt acaacagatg aaggctcagg ctagggagga gaaacatcag aagcagttgg 1021 aaagggcaca attagagaat gaaaaggaga aaagagaaat agcagaaaag gaaaaggaaa 1081 gaatagaacg tgaaaaggaa gagctaatgg aacgtctaaa acaaattgaa gagcagacaa 1141 ttaaagctca gaaagaacta gaagaacaga ctcgaaaagc tctagaactg gatcaagaac 1201 gaaaacgagc aaaagaagaa gcagaacgac ttgaaaagga gcgtcgagct gctgaagagg 1261 caaagtctgc catagcaaaa caagctgccg accagatgaa gaatcaggag cagctagcag 1321 cagaacttgc tgaattcact gccaagattg cacttctaga ggaagccaag aagaaaaagg 1381 aagaggaagc tactgagtgg caacacaaag cttttgcagc ccaggaagac ttggaaaaga 1441 ccaaagaaga gttaaaaact gtgatgtctg ccccccctcc accTCCACCA CCACCAGTCA 1501 TTCCTCCAAC AGAAAACGAA CATGATGAAC ACGATGAGAA TAATGCTGAA GCTAGTGCTG 1561 AATTATCAAA TGAAGGGGTA ATGAACCATA GAAGCGAGGA AGAACGTGTA ACCGAAACAC 1621 AGAAAAATGA GCGTGTTAAG AAGCAACTTC AGGCATTAAG TTCAGAATTA GCCCAAGCCA 1681 GAGATGAAAC CAAGAAAACA CAAAATGATG TTCTTCATGC TGAGAATGTT AAAGCAGGCC 1741 GTGATAAGTA CAAGACTCTG CGACAGATTC GACAAGGCAA TACAAAGCAG CGTATCGATG 1801 AGTTTGAAGC AATGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1861 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1921 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TTCCGTTTGG CCTCCTGAAC 1981 CAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt