Construct: ORF TRCN0000478679
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012774.1_s317c1
- Derived from:
- ccsbBroadEn_02556
- DNA Barcode:
- ACAGCGTTTCCATTCTAGACGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNPLA6 (10908)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478679
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10908 | PNPLA6 | patatin like phospholipase ... | NM_001166113.1 | 100% | 100% | |
| 2 | human | 10908 | PNPLA6 | patatin like phospholipase ... | NM_006702.5 | 100% | 100% | |
| 3 | human | 10908 | PNPLA6 | patatin like phospholipase ... | NM_001166112.1 | 97.9% | 97.9% | 1411_1412ins78;2062_2063insCAG |
| 4 | human | 10908 | PNPLA6 | patatin like phospholipase ... | NM_001166114.2 | 96.8% | 96.4% | (many diffs) |
| 5 | human | 10908 | PNPLA6 | patatin like phospholipase ... | NM_001166111.2 | 96.5% | 96.5% | 27_170del |
| 6 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | NM_015801.2 | 86.5% | 96.1% | (many diffs) |
| 7 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508825.3 | 86.5% | 96.1% | (many diffs) |
| 8 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508830.3 | 86.2% | 95.7% | (many diffs) |
| 9 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_017312890.1 | 86.2% | 95.7% | (many diffs) |
| 10 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_011242066.2 | 85.4% | 91.5% | (many diffs) |
| 11 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_017312891.1 | 85.1% | 91.1% | (many diffs) |
| 12 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508826.3 | 85% | 94.5% | (many diffs) |
| 13 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508827.3 | 85% | 94.5% | (many diffs) |
| 14 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508828.1 | 85% | 94.5% | (many diffs) |
| 15 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508829.1 | 85% | 94.5% | (many diffs) |
| 16 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | NM_001122818.2 | 84.7% | 94.2% | (many diffs) |
| 17 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508831.3 | 84.7% | 94.2% | (many diffs) |
| 18 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508822.3 | 84.4% | 93.3% | (many diffs) |
| 19 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508832.3 | 84.4% | 93% | (many diffs) |
| 20 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508824.3 | 84.1% | 93% | (many diffs) |
| 21 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XM_006508823.3 | 83.9% | 92.5% | (many diffs) |
| 22 | mouse | 50767 | Pnpla6 | patatin-like phospholipase ... | XR_378664.3 | 73.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 4047
- ORF length:
- 3981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggctccgctg caaactggaa tggtgcttgg cgtgatgatc ggggccggag 121 tggcggtggt ggtcacggcc gtgctcatcc tcctggtggt gcggaggctg cgagtgccaa 181 aaaccccagc cccggatggc ccccggtatc ggttccggaa gagggacaaa gtgctcttct 241 atggccggaa gattatgcgg aaggtgtcac aatccacctc ctccctcgtg gatacctctg 301 tctccgccac ctcccggcca cgcatgagga agaaactgaa gatgctcaac attgccaaga 361 agatcctgcg catccagaaa gagacgccca cgctgcagcg gaaggagccc ccgcccgcag 421 tgctagaagc tgacctgacc gagggcgacc tggctaactc ccatctgccc tctgaagtgc 481 tttatatgct caagaacgtc cgggtgctgg gccacttcga gaagccactc ttcctggagc 541 tctgccgcca catggtcttc cagcggctgg gccagggtga ctacgtcttc cggccgggcc 601 agccagatgc cagcatctac gtggtgcagg acgggctgct ggagctctgt ctgccagggc 661 ctgacgggaa ggagtgtgtg gtgaaggaag tggttcctgg ggacagcgtc aacagccttc 721 tcagcatcct ggatgtcatc accggtcacc agcatcccca gcggaccgtg tctgcccggg 781 cggcccggga ctccacggtg ctgcgcctgc cggtggaagc attctccgcg gtcttcacca 841 agtacccgga gagcttggtg cgggtcgtgc agatcatcat ggtgcggctg cagcgagtca 901 ccttcctggc actgcacaac tacctgggtc tgaccaatga gctcttcagc cacgagatcc 961 agcccctgcg tctgttcccc agccccggcc tcccaactcg caccagccct gtgcggggct 1021 ccaagagaat ggtcagcacc tcagctacag acgagcccag ggagacccca gggcggccac 1081 ccgatcccac cggggccccg ctgcctggac ctacagggga ccctgtgaag cccacatccc 1141 tggaaacccc ctcggcccct ctgctgagcc gctgcgtctc catgccaggg gacatctcag 1201 gcttgcaggg tggcccccgc tccgacttcg acatggccta tgagcgtggc cggatctccg 1261 tgtccctgca ggaagaggcc tccggggggt ccctggcagc ccccgctcgg acccccactc 1321 aggagcctcg tgagcagccg gcaggcgcct gtgaatacag ctactgtgag gatgagtcgg 1381 ccactggtgg ctgccctttc gggccctacc agggccgcca gaccagcagc atcttcgagg 1441 cagcaaagca ggagctggcc aagctgatgc ggattgagga cccctccctc ctgaacagca 1501 gagtcttgct gcaccacgcc aaagctggca ccatcattgc ccgccaggga gaccaggacg 1561 tgagcctgca cttcgtgctc tggggctgcc tgcacgtgta ccagcgcatg atcgacaagg 1621 cggaggacgt gtgcctgttc gtagcgcagc ccggggaact ggtggggcag ctggcggtgc 1681 tcactggcga acctctcatc ttcacactgc gagcccaacg cgactgcacc ttcctgcgga 1741 tctccaagtc cgacttctat gagatcatgc gcgcacagcc cagtgtggtg ctgagtgcgg 1801 cgcacacggt ggcagccagg atgtcgccct tcgtgcgcca gatggacttc gccatcgact 1861 ggactgcagt ggaggcggga cgcgcgctgt acaggcaggg cgaccgctcc gactgcactt 1921 acatcgtgct caatgggcgg ctgcgtagcg tgatccagcg aggcagtggc aagaaggagc 1981 tggtgggcga gtacggccgc ggcgacctca tcggcgtggt ggaggcactg acccggcagc 2041 cgcgagccac gacggtgcac gcggtgcgcg acacggagct ggccaagctt cccgagggca 2101 ccttgggtca catcaaacgc cggtacccgc aggtcgtgac ccgccttatc cacctactga 2161 gccagaaaat tctagggaat ttgcagcagc tgcaaggacc cttcccagca ggctctgggt 2221 tgggtgtgcc cccacactcg gaactcacca acccagccag caacctggca actgtggcaa 2281 tcctgcctgt gtgtgctgag gtccccatgg tggccttcac gctggagctg cagcacgccc 2341 tgcaggccat cggtccgacg ctactcctta acagtgacat catccgggca cgcctggggg 2401 cctccgcact ggatagcatc caagagttcc ggctgtcagg gtggctggcc cagcaggagg 2461 atgcacaccg tatcgtactc taccagacgg acgcctcgct gacgccctgg accgtgcgct 2521 gcctgcgaca ggccgactgc atcctcattg tgggcctggg ggaccaggag cctaccctcg 2581 gccagctgga gcagatgctg gagaacacgg ctgtgcgcgc ccttaagcag ctagtcctgc 2641 tccaccgaga ggagggcgcg ggccccacgc gcaccgtgga gtggctaaat atgcgcagct 2701 ggtgctcggg gcacctgcac ctgcgctgtc cgcgccgcct cttttcgcgc cgcagccctg 2761 ccaagctgca tgagctctac gagaaggttt tctccaggcg cgcggaccgg cacagcgact 2821 tctcccgctt ggcgagggtg ctcacgggga acaccattgc ccttgtgcta ggcgggggcg 2881 gggccagggg ctgctcgcac atcggagtac taaaggcatt agaggaggcg ggggtccccg 2941 tggacctggt gggcggcacg tccattggct ctttcatcgg agcgttgtac gcggaggagc 3001 gcagcgccag ccgcacgaag cagcgggccc gggagtgggc caagagcatg acttcggtgc 3061 tggaacctgt gttggacctc acgtacccag tcacctccat gttcactggg tctgccttta 3121 accgcagcat ccatcgggtc ttccaggata agcagattga ggacctgtgg ctgccttact 3181 tcaacgtgac cacagatatc accgcctcag ccatgcgagt ccacaaagat ggctccctgt 3241 ggcggtacgt gcgcgccagc atgacgctgt cgggctacct gcccccgctg tgcgacccca 3301 aggacgggca cctactcatg gatggcggct acatcaacaa tctgccagcg gacatcgccc 3361 gcagcatggg tgccaaaacg gtcatcgcca ttgacgtggg gagccaggat gagacggacc 3421 tcagcaccta cggggacagc ctgtccggct ggtggctgct gtggaagcgg ctgaatccct 3481 gggctgacaa ggtaaaggtt ccagacatgg ctgaaatcca gtcccgcctg gcctacgtgt 3541 cctgtgtgcg gcagctagag gttgtcaagt ccagctccta ctgcgagtac ctgcgcccgc 3601 ccatcgactg cttcaagacc atggactttg ggaagttcga ccagatctat gatgtgggct 3661 accagtacgg gaaggcggtg tttggaggct ggagccgtgg caacgtcatt gagaaaatgc 3721 TCACAGACCG GCGGTCTACA GACCTTAATG AGAGCCGCCG TGCAGACGTG CTTGCCTTCC 3781 CAAGCTCTGG CTTCACTGAC TTGGCAGAGA TTGTGTCCCG GATTGAGCCC CCCACGAGCT 3841 ATGTCTCTGA TGGCTGTGCT GACGGAGAGG AGTCAGATTG TCTGACAGAG TATGAGGAGG 3901 ACGCCGGACC CGACTGCTCG AGGGATGAAG GGGGGTCCCC CGAGGGCGCA AGCCCCAGCA 3961 CTGCCTCCGA GATGGAGGAG GAGAAGTCGA TTCTCCGGCA ACGACGCTGT CTGCCCCAGG 4021 AGCCGCCCGG CTCAGCCACA GATGCCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 4081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 4141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACAGCGTT 4201 TCCATTCTAG ACGTACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4261 aagatt