Transcript: Human NM_001166112.1

Homo sapiens patatin like phospholipase domain containing 6 (PNPLA6), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
PNPLA6 (10908)
Length:
4526
CDS:
401..4303

Additional Resources:

NCBI RefSeq record:
NM_001166112.1
NBCI Gene record:
PNPLA6 (10908)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001166112.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000238378 CGGCGGTCTACAGACCTTAAT pLKO_005 3983 CDS 100% 13.200 18.480 N PNPLA6 n/a
2 TRCN0000238376 CTCGCACATCGGAGTACTAAA pLKO_005 3148 CDS 100% 13.200 18.480 N PNPLA6 n/a
3 TRCN0000048881 GCCCTCTGAAGTGCTTTATAT pLKO.1 802 CDS 100% 15.000 10.500 N PNPLA6 n/a
4 TRCN0000238377 CTTCTATGGCCGGAAGATTAT pLKO_005 571 CDS 100% 13.200 9.240 N PNPLA6 n/a
5 TRCN0000238375 GGAAGTTCGACCAGATCTATG pLKO_005 3885 CDS 100% 10.800 7.560 N PNPLA6 n/a
6 TRCN0000048879 CGTGGCAACGTCATTGAGAAA pLKO.1 3950 CDS 100% 4.950 3.465 N PNPLA6 n/a
7 TRCN0000048880 CTGCACTTACATCGTGCTCAA pLKO.1 2170 CDS 100% 4.050 2.835 N PNPLA6 n/a
8 TRCN0000048882 GCAGAGATTGTGTCCCGGATT pLKO.1 4058 CDS 100% 4.050 2.835 N PNPLA6 n/a
9 TRCN0000048878 CTCCTGCTGCTATGCCTGTGA pLKO.1 4383 3UTR 100% 0.880 0.616 N PNPLA6 n/a
10 TRCN0000238374 GCACCGTGGAGTGGCTAAATA pLKO_005 2925 CDS 100% 15.000 9.000 N PNPLA6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001166112.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02556 pDONR223 100% 97.9% 97.9% None 1411_1412ins78;2062_2063insCAG n/a
2 ccsbBroad304_02556 pLX_304 0% 97.9% 97.9% V5 1411_1412ins78;2062_2063insCAG n/a
3 TRCN0000478679 ACAGCGTTTCCATTCTAGACGTAC pLX_317 8.4% 97.9% 97.9% V5 1411_1412ins78;2062_2063insCAG n/a
4 ccsbBroadEn_15730 pDONR223 0% 97.8% 97.8% None (many diffs) n/a
5 ccsbBroad304_15730 pLX_304 0% 97.8% 97.8% V5 (many diffs) n/a
6 TRCN0000473947 CACGTTCAACGGACCGGCACAGGT pLX_317 10.6% 97.8% 97.8% V5 (many diffs) n/a
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