Construct: ORF TRCN0000478681
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012132.1_s317c1
- Derived from:
- ccsbBroadEn_04496
- DNA Barcode:
- CCCAAGCTATTCGCCCATCTAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LHX4 (89884)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478681
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89884 | LHX4 | LIM homeobox 4 | NM_033343.4 | 100% | 100% | |
| 2 | human | 89884 | LHX4 | LIM homeobox 4 | XM_011510105.2 | 84.3% | 84.3% | 0_1ins183 |
| 3 | human | 89884 | LHX4 | LIM homeobox 4 | XM_011510106.3 | 84.3% | 84.3% | 0_1ins183 |
| 4 | human | 89884 | LHX4 | LIM homeobox 4 | XM_011510108.2 | 79.8% | 78.9% | (many diffs) |
| 5 | human | 89884 | LHX4 | LIM homeobox 4 | XM_017002755.1 | 79.8% | 78.9% | (many diffs) |
| 6 | mouse | 16872 | Lhx4 | LIM homeobox protein 4 | NM_010712.2 | 93% | 98.9% | (many diffs) |
| 7 | mouse | 16872 | Lhx4 | LIM homeobox protein 4 | XM_006529169.2 | 78.2% | 83.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1236
- ORF length:
- 1170
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gcagagtgcg actgtccccg cggaaggggc tgtcaagggg ctcccggaga 121 tgctaggtgt gccgatgcaa cagattcccc agtgcgctgg ctgcaaccag cacatcctgg 181 acaagttcat cctgaaggtc ctggacagac actggcacag ctcctgcctc aagtgtgcag 241 actgccagat gcagctggcg gacaggtgct tctccagggc tgggagcgtc tactgcaagg 301 aggacttctt caagcgcttc ggcacaaaat gcacggcctg ccagcagggt atccccccaa 361 cccaggtggt ccgcaaggcc caggactttg tctaccacct gcactgcttt gcttgcatca 421 tctgcaaccg gcagctggcc acgggggacg aattctacct catggaggac gggcggctgg 481 tgtgcaagga agactacgag acagccaagc agaacgatga ctcagaggct ggagctaagc 541 ggccccggac caccatcaca gccaagcagc tggagacatt aaagaatgca tacaagaact 601 cccccaagcc tgcccggcac gtgagggagc agctgtcctc agagacaggc ctggacatga 661 gggtcgtaca ggtttggttt cagaacagaa gggccaaaga gaaacgcctg aagaaggatg 721 cagggcggca ccgctggggg cagttctata agagcgtcaa gaggagccgg ggcagcagca 781 agcaggagaa ggagagctct gcagaggact gtgGGGTTAG TGACAGTGAG CTGAGCTTCC 841 GAGAGGATCA AATTCTCTCA GAACTTGGCC ACACCAATAG GATTTATGGC AACGTGGGGG 901 ACGTTACAGG CGGACAGTTA ATGAATGGGA GCTTCTCCAT GGACGGGACA GGACAATCCT 961 ATCAGGACTT GAGGGATGGG AGCCCCTATG GAATCCCCCA GTCTCCATCC TCCATATCGT 1021 CCCTGCCATC CCACGCTCCT TTGCTCAATG GGCTGGATTA CACGGTGGAC AGTAATTTGG 1081 GCATCATTGC GCATGCAGGG CAGGGAGTAA GCCAGACGCT GAGAGCCATG GCTGGGGGAC 1141 CCACCTCTGA CATCTCCACA GGAAGCAGTG TAGGCTATCC CGACTTTCCA ACTAGCCCAG 1201 GCTCTTGGCT CGATGAAATG GATCATCCTC CTTTTTACCC AACTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 ACCCAAGCTA TTCGCCCATC TAACGACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt