Construct: ORF TRCN0000478685
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002431.1_s317c1
- Derived from:
- ccsbBroadEn_15425
- DNA Barcode:
- GACCACTGTGATCAAGGACACGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GATA3 (2625)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478685
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2625 | GATA3 | GATA binding protein 3 | NM_002051.3 | 100% | 100% | |
| 2 | human | 2625 | GATA3 | GATA binding protein 3 | NM_001002295.2 | 99.7% | 99.7% | 777_779delAGA |
| 3 | human | 2625 | GATA3 | GATA binding protein 3 | XM_005252442.2 | 99.7% | 99.7% | 777_779delAGA |
| 4 | human | 2625 | GATA3 | GATA binding protein 3 | XM_005252443.5 | 99.7% | 99.7% | 777_779delAGA |
| 5 | mouse | 14462 | Gata3 | GATA binding protein 3 | XM_006497355.3 | 89.6% | 96.3% | (many diffs) |
| 6 | mouse | 14462 | Gata3 | GATA binding protein 3 | NM_008091.3 | 89.4% | 96.1% | (many diffs) |
| 7 | mouse | 14462 | Gata3 | GATA binding protein 3 | XM_006497354.1 | 89.4% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1395
- ORF length:
- 1329
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggtgacggcg gaccagccgc gctgggtgag ccaccaccac cccgccgtgc 121 tcaacgggca gcacccggac acgcaccacc cgggcctcag ccactcctac atggacgcgg 181 cgcagtaccc gctgccggag gaggtggatg tgctttttaa catcgacggt caaggcaacc 241 acgtcccgcc ctactacgga aactcggtca gggccacggt gcagaggtac cctccgaccc 301 accacgggag ccaggtgtgc cgcccgcctc tgcttcatgg atccctaccc tggctggacg 361 gcggcaaagc cctgggcagc caccacaccg cctccccctg gaatctcagc cccttctcca 421 agacgtccat ccaccacggc tccccggggc ccctctccgt ctaccccccg gcctcgtcct 481 cctccttgtc ggggggccac gccagcccgc acctcttcac cttcccgccc accccgccga 541 aggacgtctc cccggaccca tcgctgtcca ccccaggctc ggccggctcg gcccggcagg 601 acgagaaaga gtgcctcaag taccaggtgc ccctgcccga cagcatgaag ctggagtcgt 661 cccactcccg tggcagcatg accgccctgg gtggagcctc ctcgtcgacc caccacccca 721 tcaccaccta cccgccctac gtgcccgagt acagctccgg actcttcccc cccagcagcc 781 tgctgggcgg ctcccccacc ggcttcggat gcaagtccag gcccaaggcc cggtccagca 841 caggcaggga gtgtgtgaac tgtggggcaa cctcgacccc actgtggcgg cgagatggca 901 cgggacacta cctgtgcaac gcctgcgggc tctatcacaa aatgaacgga cagaaccggc 961 ccctcattaa gcccaagcga aggctgtctg cagccaggag agcagggacg TCCTGTGCGA 1021 ACTGTCAGAC CACCACAACC ACACTCTGGA GGAGGAATGC CAATGGGGAC CCTGTCTGCA 1081 ATGCCTGTGG GCTCTACTAC AAGCTTCACA ATATTAACAG ACCCCTGACT ATGAAGAAGG 1141 AAGGCATCCA GACCAGAAAC CGAAAAATGT CTAGCAAATC CAAAAAGTGC AAAAAAGTGC 1201 ATGACTCACT GGAGGACTTC CCCAAGAACA GCTCGTTTAA CCCGGCCGCC CTCTCCAGAC 1261 ACATGTCCTC CCTGAGCCAC ATCTCGCCCT TCAGCCACTC CAGCCACATG CTGACCACGC 1321 CCACGCCGAT GCACCCGCCA TCCAGCCTGT CCTTTGGACC ACACCACCCC TCCAGCATGG 1381 TCACCGCCAT GGGTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1441 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1501 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACCACTGTG ATCAAGGACA 1561 CGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt