Construct: ORF TRCN0000478685

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002431.1_s317c1
Derived from:
ccsbBroadEn_15425
DNA Barcode:
GACCACTGTGATCAAGGACACGCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GATA3 (2625)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478685
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2625 GATA3 GATA binding protein 3 NM_002051.3 100% 100%
2 human 2625 GATA3 GATA binding protein 3 NM_001002295.2 99.7% 99.7% 777_779delAGA
3 human 2625 GATA3 GATA binding protein 3 XM_005252442.2 99.7% 99.7% 777_779delAGA
4 human 2625 GATA3 GATA binding protein 3 XM_005252443.5 99.7% 99.7% 777_779delAGA
5 mouse 14462 Gata3 GATA binding protein 3 XM_006497355.3 89.6% 96.3% (many diffs)
6 mouse 14462 Gata3 GATA binding protein 3 NM_008091.3 89.4% 96.1% (many diffs)
7 mouse 14462 Gata3 GATA binding protein 3 XM_006497354.1 89.4% 96.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1395
ORF length:
1329
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga ggtgacggcg gaccagccgc gctgggtgag ccaccaccac cccgccgtgc
121tcaacgggca gcacccggac acgcaccacc cgggcctcag ccactcctac atggacgcgg
181cgcagtaccc gctgccggag gaggtggatg tgctttttaa catcgacggt caaggcaacc
241acgtcccgcc ctactacgga aactcggtca gggccacggt gcagaggtac cctccgaccc
301accacgggag ccaggtgtgc cgcccgcctc tgcttcatgg atccctaccc tggctggacg
361gcggcaaagc cctgggcagc caccacaccg cctccccctg gaatctcagc cccttctcca
421agacgtccat ccaccacggc tccccggggc ccctctccgt ctaccccccg gcctcgtcct
481cctccttgtc ggggggccac gccagcccgc acctcttcac cttcccgccc accccgccga
541aggacgtctc cccggaccca tcgctgtcca ccccaggctc ggccggctcg gcccggcagg
601acgagaaaga gtgcctcaag taccaggtgc ccctgcccga cagcatgaag ctggagtcgt
661cccactcccg tggcagcatg accgccctgg gtggagcctc ctcgtcgacc caccacccca
721tcaccaccta cccgccctac gtgcccgagt acagctccgg actcttcccc cccagcagcc
781tgctgggcgg ctcccccacc ggcttcggat gcaagtccag gcccaaggcc cggtccagca
841caggcaggga gtgtgtgaac tgtggggcaa cctcgacccc actgtggcgg cgagatggca
901cgggacacta cctgtgcaac gcctgcgggc tctatcacaa aatgaacgga cagaaccggc
961ccctcattaa gcccaagcga aggctgtctg cagccaggag agcagggacg TCCTGTGCGA
1021ACTGTCAGAC CACCACAACC ACACTCTGGA GGAGGAATGC CAATGGGGAC CCTGTCTGCA
1081ATGCCTGTGG GCTCTACTAC AAGCTTCACA ATATTAACAG ACCCCTGACT ATGAAGAAGG
1141AAGGCATCCA GACCAGAAAC CGAAAAATGT CTAGCAAATC CAAAAAGTGC AAAAAAGTGC
1201ATGACTCACT GGAGGACTTC CCCAAGAACA GCTCGTTTAA CCCGGCCGCC CTCTCCAGAC
1261ACATGTCCTC CCTGAGCCAC ATCTCGCCCT TCAGCCACTC CAGCCACATG CTGACCACGC
1321CCACGCCGAT GCACCCGCCA TCCAGCCTGT CCTTTGGACC ACACCACCCC TCCAGCATGG
1381TCACCGCCAT GGGTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1441CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1501TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACCACTGTG ATCAAGGACA
1561CGCCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)