Construct: ORF TRCN0000478695

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002453.1_s317c1
Derived from:
ccsbBroadEn_08756
DNA Barcode:
GAGCTGGGGACCGAGGGTTTTGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RNF213 (57674)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478695
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57674 RNF213 ring finger protein 213 NM_020954.4 99.8% 99.8% (many diffs)
2 human 57674 RNF213 ring finger protein 213 XM_011525087.3 27.3% 26.7% (many diffs)
3 human 57674 RNF213 ring finger protein 213 XM_011525086.2 24.9% 24.3% (many diffs)
4 human 57674 RNF213 ring finger protein 213 XM_011525084.2 21.7% 21.1% (many diffs)
5 human 57674 RNF213 ring finger protein 213 NM_001256071.3 20.1% 19.6% (many diffs)
6 human 57674 RNF213 ring finger protein 213 XM_005257545.4 19.9% 19.4% (many diffs)
7 human 57674 RNF213 ring finger protein 213 XM_005257546.4 19.9% 19.4% (many diffs)
8 human 57674 RNF213 ring finger protein 213 XM_006721995.3 19.9% 19.4% (many diffs)
9 human 57674 RNF213 ring finger protein 213 XM_017024905.2 14.5% 13.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3255
ORF length:
3189
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gtgtccttcg tgccagcatg tctccaagga ggaaaccccc aagttctgca
121gccagtgcgg agagaggctg cctcctgcag cccccatagc agattctgag aacaataact
181ccacaatggc gtcggcctcg gagggtgaaa tggagtgtgg gcaggagctg aaggaggaag
241ggggcccgtg cttgttcccg ggctcagaca gttggcaaga aaaccccgag gagccctgtt
301ccaaagcctc ctggaccgtc caagaaagca aaaagaagaa aaggaagaag aaaaagaagg
361ggaacaagtc cgcttcctca gagctggctt ccttgcccct ttctcctgcc agcccctgtc
421acctgacttt gctttcaaac ccatggcctc aggacacagc cctgccccac agccaagccc
481agcagagtgg ccccactggc cagccgagcc agcccccggg cacagccacc acgccactgg
541agggtgacgg cctctccgcg cccaccgagg ttggcgacag ccccctgcag gcccaggctt
601tgggagaggc aggagtggcc acaggaagtg aggctcagag cagcccgcaa ttccaggacc
661acacggaagg ggaggaccag gacgcttcca tcccctctgg gggcagaggc ctgtcccagg
721aggggaccgg tccccccacc tctgctggtg aaggccattc taggactgaa gatgctgccc
781aggagctcct gttgcctgag tcaaaaggag gcagctctga gcccgggaca gaactgcaga
841ccaccgagca acaggcaggg gcctcagcct ctacggcagt tgatgctgta gctgagccag
901ccaatgcagt taaaggggcc gggaaggaaa tgaaagagaa gacccagaga atgaaacagc
961caccagcaac cactcctcct ttcaaaacac actgccagga agctgagacc aagaccaagg
1021acgagacggc tgctgctgaa gaaaaagtcg gtaaaaatga acaaggggag cctgaagacc
1081tcaagaagcc agaggggaag aacagaagtg cagctgctgt gaaaaacgag aaggagcaaa
1141aaaaccagga agcagatgtc caggaagtga aggcaagcac gctgagcccg ggtggaggag
1201tcaccgtgtt cttccacgcc atcatctctc ttcatttccc attcaatcct gacctccata
1261aagtcttcat cagaggagga gaagaatttg gggagtcaaa atgggacagc aatatctgtg
1321agctgcacta caccagagac ttgggtcatg accgcgttct tgttgaaggc attgtctgca
1381tttccaagaa gcacctagat aaatacattc cttacaagta cgtcatttat aatggggaat
1441cttttgagta tgagttcatt tacaagcacc agcagaagaa gggcgagtac gtcaaccgct
1501gtctgttcat aaaatcttca cttctgggct caggagactg gcatcagtac tatgacatag
1561tttatatgaa gcctcatggg agactccaga aagtcatgaa ccacatcaca gacgggccga
1621ggaaggacct ggtgaagggg aagcagattg ccgctgcgct catgctggac agcaccttca
1681gcatcctgca gacctgggac accatcaacc tgaacagctt cttcacccag ttcgagcagt
1741tttgctttgt cctgcaacag cctatgattt atgaaggaca ggcacagctg tggaccgatt
1801tgcagtacag ggagaaagag gtgaagagat acctgtggca acatctgaaa aaacacgtgg
1861taccattgcc ggacggaaaa agcacggact ttttgcctgt ggactgccca gtgaggagta
1921aactgaaaac aggcctgatt gtcctttttg tagtggaaaa aattgagctt ttattagaag
1981gcagcctgga ctggttgtgt cacctcctaa cctcagatgc cagctcacca gatgagtttc
2041accgtgacct aagccacatc cttgggatac ctcagagctg gcggctgtac ctggtgaacc
2101tgtgccaaag atgcatggac acaaggacgt acacctggct gggcgccctg cctgtcctgc
2161actgctgtat ggagctggcc ccgcggcaca aggatgcctg gagacagcct gaggacacct
2221gggccgctct ggagggactc tccttctcac cgttccggga acaaatgcta gatacgagtt
2281ccctacttca gtttatgaga gagaagcagc atttgctgag catagacgag cctctcttcc
2341ggtcctggtt tagtctgcta cctctgagtc acctggttat gtatatggaa aacttcattg
2401agcacctggg tcgttttcct gctcatatcc tggactgtct ttcagggatt tactaccggc
2461ttccgggact tgagcaagtc ttgaatacgc aggatgttca ggatgttcag aacgttcaga
2521acattttaga aatgctgttg cgactcctgg acacttaccg ggacaagatt cccgaggagg
2581ccttgtcacc atcctacctg actgtgtgtc tgaaactgca tgaagccatc tgcagcagca
2641caaagctact taagttttac gagctgccag ccttatctgc cgagattgtc tgcagaatga
2701ttagacttct atctctggtg gattctgcag gacagagaga tgaaactgga aataattcag
2761tccaaacagt cttccaaggg acccttgctg ctacgaaaag gtggctccga gaagttttta
2821caaagaacat gctcacatct tcaggtgcct cattcacata cgtcaaggaa attgaggtct
2881ggaggcggct ggtggaaatc caattcccCG CGGAGCATGG CTGGAAGGAG TCGTTGCTGG
2941GAGACATGGA ATGGAGGCTC ACAAAGGAGG AACCCCTCTC CCAGATCACT GCCTACTGCA
3001ATAGTTGCTG GGACACCAAA GGCTTAGAGG ACAGTGTGGC CAAGACCTTC GAGAAATGCA
3061TCATTGAAGC CGTGAGCTCA GCCTGCCAGG TGAACAATCT CTCCTCCTGG GAAACGGATT
3121CGGGCTCACA GCTGTGTTCT GCCATGACCC AGCTAAGGGC TATGAAGCAC CCGCTGGGTC
3181TCAGCTCCTC CGCTAACTCA GAGATTGGGA AGTGGGCACC CTCCTCCCTC GCCAAGGGCA
3241ATGGCGCTGA AATCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
3301CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
3361TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GAGCTGGGGA CCGAGGGTTT
3421TGCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)