Construct: ORF TRCN0000478695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002453.1_s317c1
- Derived from:
- ccsbBroadEn_08756
- DNA Barcode:
- GAGCTGGGGACCGAGGGTTTTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF213 (57674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478695
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57674 | RNF213 | ring finger protein 213 | NM_020954.4 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 57674 | RNF213 | ring finger protein 213 | XM_011525087.3 | 27.3% | 26.7% | (many diffs) |
| 3 | human | 57674 | RNF213 | ring finger protein 213 | XM_011525086.2 | 24.9% | 24.3% | (many diffs) |
| 4 | human | 57674 | RNF213 | ring finger protein 213 | XM_011525084.2 | 21.7% | 21.1% | (many diffs) |
| 5 | human | 57674 | RNF213 | ring finger protein 213 | NM_001256071.3 | 20.1% | 19.6% | (many diffs) |
| 6 | human | 57674 | RNF213 | ring finger protein 213 | XM_005257545.4 | 19.9% | 19.4% | (many diffs) |
| 7 | human | 57674 | RNF213 | ring finger protein 213 | XM_005257546.4 | 19.9% | 19.4% | (many diffs) |
| 8 | human | 57674 | RNF213 | ring finger protein 213 | XM_006721995.3 | 19.9% | 19.4% | (many diffs) |
| 9 | human | 57674 | RNF213 | ring finger protein 213 | XM_017024905.2 | 14.5% | 13.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3255
- ORF length:
- 3189
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gtgtccttcg tgccagcatg tctccaagga ggaaaccccc aagttctgca 121 gccagtgcgg agagaggctg cctcctgcag cccccatagc agattctgag aacaataact 181 ccacaatggc gtcggcctcg gagggtgaaa tggagtgtgg gcaggagctg aaggaggaag 241 ggggcccgtg cttgttcccg ggctcagaca gttggcaaga aaaccccgag gagccctgtt 301 ccaaagcctc ctggaccgtc caagaaagca aaaagaagaa aaggaagaag aaaaagaagg 361 ggaacaagtc cgcttcctca gagctggctt ccttgcccct ttctcctgcc agcccctgtc 421 acctgacttt gctttcaaac ccatggcctc aggacacagc cctgccccac agccaagccc 481 agcagagtgg ccccactggc cagccgagcc agcccccggg cacagccacc acgccactgg 541 agggtgacgg cctctccgcg cccaccgagg ttggcgacag ccccctgcag gcccaggctt 601 tgggagaggc aggagtggcc acaggaagtg aggctcagag cagcccgcaa ttccaggacc 661 acacggaagg ggaggaccag gacgcttcca tcccctctgg gggcagaggc ctgtcccagg 721 aggggaccgg tccccccacc tctgctggtg aaggccattc taggactgaa gatgctgccc 781 aggagctcct gttgcctgag tcaaaaggag gcagctctga gcccgggaca gaactgcaga 841 ccaccgagca acaggcaggg gcctcagcct ctacggcagt tgatgctgta gctgagccag 901 ccaatgcagt taaaggggcc gggaaggaaa tgaaagagaa gacccagaga atgaaacagc 961 caccagcaac cactcctcct ttcaaaacac actgccagga agctgagacc aagaccaagg 1021 acgagacggc tgctgctgaa gaaaaagtcg gtaaaaatga acaaggggag cctgaagacc 1081 tcaagaagcc agaggggaag aacagaagtg cagctgctgt gaaaaacgag aaggagcaaa 1141 aaaaccagga agcagatgtc caggaagtga aggcaagcac gctgagcccg ggtggaggag 1201 tcaccgtgtt cttccacgcc atcatctctc ttcatttccc attcaatcct gacctccata 1261 aagtcttcat cagaggagga gaagaatttg gggagtcaaa atgggacagc aatatctgtg 1321 agctgcacta caccagagac ttgggtcatg accgcgttct tgttgaaggc attgtctgca 1381 tttccaagaa gcacctagat aaatacattc cttacaagta cgtcatttat aatggggaat 1441 cttttgagta tgagttcatt tacaagcacc agcagaagaa gggcgagtac gtcaaccgct 1501 gtctgttcat aaaatcttca cttctgggct caggagactg gcatcagtac tatgacatag 1561 tttatatgaa gcctcatggg agactccaga aagtcatgaa ccacatcaca gacgggccga 1621 ggaaggacct ggtgaagggg aagcagattg ccgctgcgct catgctggac agcaccttca 1681 gcatcctgca gacctgggac accatcaacc tgaacagctt cttcacccag ttcgagcagt 1741 tttgctttgt cctgcaacag cctatgattt atgaaggaca ggcacagctg tggaccgatt 1801 tgcagtacag ggagaaagag gtgaagagat acctgtggca acatctgaaa aaacacgtgg 1861 taccattgcc ggacggaaaa agcacggact ttttgcctgt ggactgccca gtgaggagta 1921 aactgaaaac aggcctgatt gtcctttttg tagtggaaaa aattgagctt ttattagaag 1981 gcagcctgga ctggttgtgt cacctcctaa cctcagatgc cagctcacca gatgagtttc 2041 accgtgacct aagccacatc cttgggatac ctcagagctg gcggctgtac ctggtgaacc 2101 tgtgccaaag atgcatggac acaaggacgt acacctggct gggcgccctg cctgtcctgc 2161 actgctgtat ggagctggcc ccgcggcaca aggatgcctg gagacagcct gaggacacct 2221 gggccgctct ggagggactc tccttctcac cgttccggga acaaatgcta gatacgagtt 2281 ccctacttca gtttatgaga gagaagcagc atttgctgag catagacgag cctctcttcc 2341 ggtcctggtt tagtctgcta cctctgagtc acctggttat gtatatggaa aacttcattg 2401 agcacctggg tcgttttcct gctcatatcc tggactgtct ttcagggatt tactaccggc 2461 ttccgggact tgagcaagtc ttgaatacgc aggatgttca ggatgttcag aacgttcaga 2521 acattttaga aatgctgttg cgactcctgg acacttaccg ggacaagatt cccgaggagg 2581 ccttgtcacc atcctacctg actgtgtgtc tgaaactgca tgaagccatc tgcagcagca 2641 caaagctact taagttttac gagctgccag ccttatctgc cgagattgtc tgcagaatga 2701 ttagacttct atctctggtg gattctgcag gacagagaga tgaaactgga aataattcag 2761 tccaaacagt cttccaaggg acccttgctg ctacgaaaag gtggctccga gaagttttta 2821 caaagaacat gctcacatct tcaggtgcct cattcacata cgtcaaggaa attgaggtct 2881 ggaggcggct ggtggaaatc caattcccCG CGGAGCATGG CTGGAAGGAG TCGTTGCTGG 2941 GAGACATGGA ATGGAGGCTC ACAAAGGAGG AACCCCTCTC CCAGATCACT GCCTACTGCA 3001 ATAGTTGCTG GGACACCAAA GGCTTAGAGG ACAGTGTGGC CAAGACCTTC GAGAAATGCA 3061 TCATTGAAGC CGTGAGCTCA GCCTGCCAGG TGAACAATCT CTCCTCCTGG GAAACGGATT 3121 CGGGCTCACA GCTGTGTTCT GCCATGACCC AGCTAAGGGC TATGAAGCAC CCGCTGGGTC 3181 TCAGCTCCTC CGCTAACTCA GAGATTGGGA AGTGGGCACC CTCCTCCCTC GCCAAGGGCA 3241 ATGGCGCTGA AATCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3301 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3361 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GAGCTGGGGA CCGAGGGTTT 3421 TGCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt