Construct: ORF TRCN0000478712

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004563.1_s317c1
Derived from:
ccsbBroadEn_03253
DNA Barcode:
GCTTTATTCTGTTAGGATTAGCGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PAIP2 (51247)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478712
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51247 PAIP2 poly(A) binding protein int... NM_001033112.2 100% 100%
2 human 51247 PAIP2 poly(A) binding protein int... NM_016480.5 100% 100%
3 human 51247 PAIP2 poly(A) binding protein int... XM_011543428.1 100% 100%
4 human 51247 PAIP2 poly(A) binding protein int... XM_017009522.1 100% 100%
5 human 51247 PAIP2 poly(A) binding protein int... XM_017009523.2 100% 100%
6 human 51247 PAIP2 poly(A) binding protein int... NR_109830.1 25.1% 1_175del;313_314insATAG;553_1494del
7 mouse 67869 Paip2 polyadenylate-binding prote... NM_026420.2 95.8% 96% (many diffs)
8 mouse 67869 Paip2 polyadenylate-binding prote... XM_011246987.2 95.8% 96% (many diffs)
9 mouse 67869 Paip2 polyadenylate-binding prote... XM_011246988.2 95.8% 96% (many diffs)
10 mouse 67869 Paip2 polyadenylate-binding prote... XM_011246989.1 95.8% 96% (many diffs)
11 mouse 67869 Paip2 polyadenylate-binding prote... XM_011246990.2 95.8% 96% (many diffs)
12 mouse 67869 Paip2 polyadenylate-binding prote... XM_017317972.1 95.8% 96% (many diffs)
13 mouse 67869 Paip2 polyadenylate-binding prote... XM_017317973.1 95.8% 96% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
79
ORF end:
460
ORF length:
381
Sequence:
1TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT
61TTGTACAAAA AAGTTGGCAT GAAAGATCCA AGTCGCAGCA GTACTAGCCC AAGCATCATC
121AATGAAGATG TGATTATTAA CGGTCATTCT CATGAAGATG ACAATCCATT TGCAGAGTAC
181ATGTGGATGG AAAATGAAGA AGAATTCAAC AGACAAATAG AAGAGGAGTT ATGGGAAGAA
241GAATTTATTG AACGCTGTTT CCAAGAAATG CTGGAAGAGG AAGAAGAGCA TGAATGGTTT
301ATTCCAGCTC GAGATCTCCC ACAAACTATG GACCAAATCC AAGACCAGTT TAATGACCTT
361GTTATCAGTG ATGGCTCTTC TCTGGAAGAT CTTGTGGTCA AGAGCAATCT GAATCCAAAT
421GCAAAGGAGT TTGTTCCTGG GGTGAAGTAC GGAAATATTT GCCCAACTTT CTTGTACAAA
481GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA
541TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG
601GACGAGCTTT ATTCTGTTAG GATTAGCGCA CGCGTTAAGT Cgacaatcaa cctctggatt
661acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)