Construct: ORF TRCN0000478731
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018102.2_s317c1
- Derived from:
- ccsbBroadEn_14061
- DNA Barcode:
- ATGAAATGAAAACGAACCGCTTCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF3A (11127)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478731
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11127 | KIF3A | kinesin family member 3A | NM_001300792.2 | 99.8% | 99.4% | (many diffs) |
| 2 | human | 11127 | KIF3A | kinesin family member 3A | NM_007054.7 | 99.4% | 99.1% | (many diffs) |
| 3 | human | 11127 | KIF3A | kinesin family member 3A | XM_017008995.2 | 97.5% | 97% | (many diffs) |
| 4 | human | 11127 | KIF3A | kinesin family member 3A | XM_017008996.2 | 97.1% | 96.7% | (many diffs) |
| 5 | human | 11127 | KIF3A | kinesin family member 3A | NM_001300791.2 | 96.5% | 96% | (many diffs) |
| 6 | human | 11127 | KIF3A | kinesin family member 3A | XM_006714526.4 | 94.3% | 93.6% | (many diffs) |
| 7 | mouse | 16568 | Kif3a | kinesin family member 3A | NM_008443.4 | 87.6% | 97.1% | (many diffs) |
| 8 | mouse | 16568 | Kif3a | kinesin family member 3A | NM_001290805.1 | 87.2% | 96.8% | (many diffs) |
| 9 | mouse | 16568 | Kif3a | kinesin family member 3A | NM_001290806.1 | 86.7% | 96.1% | (many diffs) |
| 10 | mouse | 16568 | Kif3a | kinesin family member 3A | XM_006532326.3 | 85% | 93.9% | (many diffs) |
| 11 | mouse | 16568 | Kif3a | kinesin family member 3A | XM_006532325.3 | 84.7% | 93.8% | (many diffs) |
| 12 | mouse | 16568 | Kif3a | kinesin family member 3A | XM_006532328.3 | 79.6% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2172
- ORF length:
- 2106
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gatcaataaa tcagagaagc cagaaagctg cgataatgtg aaggttgttg 121 ttaggtgccg gcccctcaat gagagagaga aatcaatgtg ctacaaacag gctgtcagtg 181 tggatgagat gaggggaact atcactgtac ataagactga ttcttccaat gaacctccaa 241 agacatttac ttttgatact gtttttggac cagagagtaa acaacttgat gtttataact 301 taactgcaag acctattatt gattctgtac ttgaaggcta caatgggact atttttgcat 361 atggacaaac cggaacaggc aaaactttta ccatggaagg tgttcgagct attcctgaac 421 ttagaggaat aattcccaat tcatttgctc acatatttgg tcatattgca aaagcggagg 481 gtgatacaag atttttggtt cgagtgtctt atttgggaat atataatgaa gaagttcgtg 541 accttttggg caaggatcag acacaaaggt tagaggttat agaaagacct gatgtgggag 601 tttatatcaa agatttatca gcttatgtgg taaataatgc tgatgatatg gatagaatta 661 tgacgctagg ccacaaaaat cgttctgttg gtgcaactaa tatgaacgaa catagttccc 721 gttcccatgc catctttaca attactatag aatgcagtga aaaaggcatt gatggtaaca 781 tgcatgtcag gatggggaag ctccatcttg tagatcttgc tggttcagaa agacaggcaa 841 aaactggagc tactggacag cgcctaaagg aagctacaaa aatcaatctt tcactttcca 901 cccttggtaa tgtaatttct gccttggttg atggaaaaag cactcatgtg ccttatcgta 961 actctaaact gactcgtctt cttcaggatt ccttaggagg aaattcaaaa accatgatgt 1021 gtgcaaatat tgggccagca gattacaatt atgatgaaac tatcagtaca ttacggtatg 1081 ccaatcgtgc taagaatatt aaaaataaag ctagaattaa tgaagatcca aaggatgctt 1141 tgctgcgtca gttccagaaa gaaatagaag aactgaaaaa gaagcttgaa gaaggagaag 1201 aaatatcagg ctctgatatc agtgggtcag aggaagatga tgatgaagag ggtgaggttg 1261 gagaagatgg agagaaaagg aaaaaaagaa gggatcaaac aggaaaaaag aaagtctccc 1321 cagacaagat gattgaaatg caagcaaaaa ttgatgagga gagaaaagca cttgaaacaa 1381 agctcgacat ggaagaagaa gaaagaaaca aggctagagc tgaattagag aaacgggaaa 1441 aagatcttct taaagcccaa caagagcatc agtctttgct ggaaaaatta tctgccctgg 1501 aaaagaaggt aattgttggt ggggttgact tgttggccaa agctgaggaa caagagaaac 1561 ttcttgaaga atctaacatg gaactggaag aaaggaggaa aagagcagag caacttcgca 1621 gagaacttga ggaaaaagag caagaacgct tggatattga agaaaaatat accagtttgc 1681 aagaggaagc acagggaaag accaagaagt taaagaaagt ttggactatg ctgatggctg 1741 caaagtcaga gatggctgat ctccaacaag aacatcagag ggaaattgaa ggcctactgg 1801 agaacattcg gcaacttagc cgggagcttc gacttcagat gcttattatt gataacttta 1861 taccTCGGGA TTATCAGGAA ATGATTGAAA ACTATGTCCA TTGGAATGAA GACATAGGAG 1921 AATGGCAGCT AAAATGTGTT GCTTATACAG GAAATAACAT GAGGAAGCAA ACCCCAGTAC 1981 CTGATAAAAA GGAGAAAGAT CCCTTTGAGG TGGACCTTTC TCACGTGTAT CTTGCCTATA 2041 CTGAGGAGAG TCTGCGTCAG TCTTTGATGA AACTAGAAAG ACCACGAACT TCAAAGGGGA 2101 AAGCAAGGCC AAAGACAGGG AGAAGAAAGC GTTCTGCAAA GCCTGAAACT GTAATTGACT 2161 CTTTACTGAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 2221 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 2281 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATGA AATGAAAACG AACCGCTTCC 2341 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt