Construct: ORF TRCN0000478736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012600.1_s317c1
- Derived from:
- ccsbBroadEn_11517
- DNA Barcode:
- ACCTAGGCACAACGCTACTCATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PITRM1 (10531)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478736
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347728.2 | 88.5% | 88.5% | 1_204del;993G>A;1805A>G |
| 2 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347727.2 | 64.1% | 64% | 1_894del;1683G>A;2495A>G |
| 3 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347726.2 | 56.1% | 56.1% | (many diffs) |
| 4 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347729.1 | 51.8% | 51.7% | 1_1485del;2274G>A;3086A>G |
| 5 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_014889.4 | 51.4% | 51.3% | 1_1509del;2298G>A;3110A>G |
| 6 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001242307.1 | 51.3% | 51.3% | (many diffs) |
| 7 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001242309.1 | 46.5% | 46.4% | (many diffs) |
| 8 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747001.2 | 45.9% | (many diffs) | |
| 9 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347730.1 | 45.4% | 45.3% | (many diffs) |
| 10 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NM_001347725.2 | 45% | 45% | (many diffs) |
| 11 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NR_144638.2 | 43.7% | (many diffs) | |
| 12 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747000.1 | 43.7% | (many diffs) | |
| 13 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NR_144641.2 | 43.7% | (many diffs) | |
| 14 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NR_144640.2 | 43.2% | (many diffs) | |
| 15 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | NR_144639.2 | 43.1% | (many diffs) | |
| 16 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747002.2 | 43% | (many diffs) | |
| 17 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747003.2 | 40.2% | (many diffs) | |
| 18 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001746999.2 | 38% | (many diffs) | |
| 19 | human | 10531 | PITRM1 | pitrilysin metallopeptidase 1 | XR_001747004.2 | 37.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1668
- ORF length:
- 1602
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gccagatgac aagtatcacg agaagcaggc acaggtggaa gccacgaagc 121 tcaagcagaa ggtcgaggct ctgtcccccg gagacaggca gcagatctac gagaaaggtc 181 tagaattacg gagtcaacaa agcaaacctc aagatgcctc ttgtctgcca gcgttgaaag 241 tttccgatat tgaacccacc atacctgtca cagagttgga cgtggtcctg acagctggag 301 atatccctgt tcagtactgc gcccagccca ccaatggcat ggtgtatttc cgggccttct 361 ccagcctgaa cacactcccc gaggagctga ggccctatgt gcccctcttc tgcagcgtcc 421 tcaccaagct gggctgcggc cttcttgact accgggagca ggctcagcag atagaattga 481 agaccggagg gatgagtgct tctccccacg tgctccccga cgactcacac atggacacct 541 acgagcaggg tgtgcttttc tcctctctct gcctggatcg aaacctgcca gacatgatgc 601 agctatggag tgaaatattt aacaacccgt gctttgaaga agaggagcac ttcaaggtgc 661 tggtgaagat gaccgcccag gagctcgcca atggaattcc tgactctggg cacctgtacg 721 catccatcag ggcaggccgg accctcacgc ccgcagggga cctgcaggag accttcagcg 781 ggatggatca ggtgcggctg atgaagagga ttgcagaaat gacagatatc aaacccatcc 841 tgaggaagct cccacgtatc aagaaacact tgttaaatgg tgataatatg aggtgttcag 901 tgaatgcgac tcctcagcag atgcctcaga cagaaaaagc ggtcgaagac ttccttagaa 961 gcatcggtcg gagtaaaaag gaacggaggc ctgtgcgccc acacacggtc gagaaacctg 1021 tgcccagcag ctctggtgga gatgcccacg ttccccatgg ctcccaggtc attaggaagc 1081 tggtcatgga acccaccttc aagccctggc agatgaagac tcacttcctg atgcccttcc 1141 cggtgaatta cgtgggtgaa tgcatccgaa ctgtccccta cacggaccca gatcatgcca 1201 gtcttaaaaT CCTTGCACGT TTGATGACTG CCAAATTCTT GCATACAGAA ATTCGAGAAA 1261 AAGGCGGTGC TTATGGTGGA GGCGCAAAAC TCAGCCACAA TGGGATTTTC ACCCTTTACT 1321 CTTACAGGGA CCCAAATACA ATAGAGACGC TCCAGTCTTT TGGGAAGGCT GTCGACTGGG 1381 CTAAGTCTGG AAAATTCACA CAGCAAGACA TCGACGAAGC CAAACTTTCT GTCTTCTCAA 1441 CCGTAGATGC TCCTGTCGCT CCTTCAGACA AAGGAATGGA CCACTTCTTG TACGGCCTCT 1501 CGGATGAGAT GAAGCAGGCC CACAGAGAGC AGCTCTTTGC TGTCAGCCAC GACAAGCTCC 1561 TGGCCGTGAG CGATAGATAC CTCGGCACTG GGAAGAGCAC ACACGGCCTG GCCATCCTCG 1621 GACCCGAGAA CCCGAAAATT GCCAAGGACC CATCCTGGAT CATCCGATGC CCAACTTTCT 1681 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1741 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1801 GTGGAAAGGA CGAACCTAGG CACAACGCTA CTCATCAACG CGTTAAGTCg acaatcaacc 1861 tctggattac aaaatttgtg aaagatt