Construct: ORF TRCN0000478746
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018320.1_s317c1
- Derived from:
- ccsbBroadEn_02046
- DNA Barcode:
- CCGAATCTTCATATAACAGCATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPNE1 (8904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478746
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8904 | CPNE1 | copine 1 | NM_152925.3 | 100% | 100% | |
| 2 | human | 8904 | CPNE1 | copine 1 | NM_152926.3 | 100% | 100% | |
| 3 | human | 8904 | CPNE1 | copine 1 | NM_152927.3 | 100% | 100% | |
| 4 | human | 8904 | CPNE1 | copine 1 | NM_152928.3 | 100% | 100% | |
| 5 | human | 8904 | CPNE1 | copine 1 | NM_001198863.2 | 99.8% | 99.8% | 1236_1237insCAA |
| 6 | human | 8904 | CPNE1 | copine 1 | NM_003915.5 | 99% | 99% | 1_15del |
| 7 | human | 8904 | CPNE1 | copine 1 | NR_037188.2 | 75% | 1_337del;1949_2148del | |
| 8 | mouse | 266692 | Cpne1 | copine I | NM_170588.3 | 86.4% | 91.4% | (many diffs) |
| 9 | mouse | 266692 | Cpne1 | copine I | NM_170590.3 | 86.4% | 91.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1677
- ORF length:
- 1611
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccactgcgtg accttggttc agctgtccat ttcctgtgac catctcattg 121 acaaggacat cggctccaag tctgacccac tctgcgtcct tttacaggat gtgggagggg 181 gcagctgggc tgagcttggc cggactgaac gggtgcggaa ctgctcaagc cctgagttct 241 ccaagactct acagcttgag taccgctttg agacagtcca gaagctacgc tttggaatct 301 atgacataga caacaagacg ccagagctga gggatgatga cttcctaggg ggtgctgagt 361 gttccctagg acagattgtg tccagccagg tactgactct ccccttgatg ctgaagcctg 421 gaaaacctgc tgggcggggg accatcacgg tctcagctca ggaattaaag gacaatcgtg 481 tagtaaccat ggaggtagag gccagaaacc tagataagaa ggacttcctg ggaaaatcag 541 atccatttct ggagttcttc cgccagggtg atgggaaatg gcacctggtg tacagatctg 601 aggtcatcaa gaacaacctg aaccctacat ggaagcgttt ctcagtcccc gttcagcatt 661 tctgtggtgg gaaccccagc acacccatcc aggtgcaatg ctccgattat gacagtgacg 721 ggtcacatga tctcatcggt accttccaca ccagcttggc ccagctgcag gcagtcccgg 781 ctgagtttga atgcatccac cctgagaagc agcagaaaaa gaaaagctac aagaactctg 841 gaactatccg tgtcaagatt tgtcgggtag aaacagagta ctcctttctg gactatgtga 901 tgggaggctg tcagatcaac ttcactgtgg gcgtggactt cactggctcc aatggagacc 961 cctcctcacc tgactcccta cactacctga gtccaacagg ggtcaatgag tacctgatgg 1021 cactgtggag tgtgggcagc gtggttcagg actatgactc agacaagctg ttccctgcat 1081 ttggatttgg ggcccaggtt ccccctgact ggcaggtctc gcatgaattt gccttgaatt 1141 tcaaccccag taacccctac tgtgcaggca tccagggcat tgtggatgcc taccgccaag 1201 ccctgcccca agttcgccTC TATGGCCCTA CCAACTTTGC ACCCATCATC AACCATGTGG 1261 CCAGGTTTGC AGCCCAGGCT GCACATCAGG GGACTGCCTC GCAATACTTC ATGCTGTTGC 1321 TGCTGACTGA TGGTGCTGTG ACGGATGTGG AAGCCACACG TGAGGCTGTG GTGCGTGCCT 1381 CGAACCTGCC CATGTCAGTG ATCATTGTGG GTGTGGGTGG TGCTGACTTT GAGGCCATGG 1441 AGCAGCTGGA CGCTGATGGT GGACCCCTGC ATACACGTTC TGGGCAGGCT GCTGCCCGCG 1501 ACATTGTGCA GTTTGTACCC TACCGCCGGT TCCAGAATGC CCCTCGGGAG GCATTGGCAC 1561 AGACCGTGCT CGCAGAAGTG CCCACACAAC TGGTCTCATA CTTCAGGGCC CAGGGTTGGG 1621 CCCCGCTCAA GCCACTTCCA CCCTCAGCCA AGGATCCTGC ACAGGCCCCC CAGGCCTACC 1681 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1741 TCGATTCTAC GTAGTAATGA ACTAGCCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1801 ATATATCTTG TGGAAAGGAC GACCGAATCT TCATATAACA GCATCTACGC GTTAAGTCga 1861 caatcaacct ctggattaca aaatttgtga aagatt