Transcript: Mouse NM_170588.3

Mus musculus copine I (Cpne1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Cpne1 (266692)
Length:
3542
CDS:
230..1840

Additional Resources:

NCBI RefSeq record:
NM_170588.3
NBCI Gene record:
Cpne1 (266692)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_170588.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416967 TAGATCCCTTTGGTCTAATAA pLKO_005 2097 3UTR 100% 15.000 21.000 N Cpne1 n/a
2 TRCN0000111600 GCCTGGAGTATAGTCTTGATT pLKO.1 2441 3UTR 100% 5.625 7.875 N Cpne1 n/a
3 TRCN0000111603 GCAGATAGAATACCACTTTGA pLKO.1 412 CDS 100% 4.950 6.930 N Cpne1 n/a
4 TRCN0000429699 GAAGCTAGAAACCTAGATAAG pLKO_005 659 CDS 100% 10.800 8.640 N Cpne1 n/a
5 TRCN0000007032 CGCTTTGGAATCTATGACATA pLKO.1 449 CDS 100% 4.950 3.960 N CPNE1 n/a
6 TRCN0000320429 CGCTTTGGAATCTATGACATA pLKO_005 449 CDS 100% 4.950 3.960 N CPNE1 n/a
7 TRCN0000421380 CACCTGACTCCCTGCATTATC pLKO_005 1128 CDS 100% 13.200 9.240 N Cpne1 n/a
8 TRCN0000429848 GAGGCTGCCAGATCAACTTTA pLKO_005 1065 CDS 100% 13.200 9.240 N Cpne1 n/a
9 TRCN0000111604 GACAAGCTATTTCCTGCATTT pLKO.1 1223 CDS 100% 10.800 7.560 N Cpne1 n/a
10 TRCN0000111602 CCTACAGGAGTCAACGAGTAT pLKO.1 1154 CDS 100% 4.950 3.465 N Cpne1 n/a
11 TRCN0000111601 CCTAGGGAAATCTGACCCATT pLKO.1 688 CDS 100% 4.050 2.835 N Cpne1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_170588.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02046 pDONR223 100% 86.4% 91.4% None (many diffs) n/a
2 ccsbBroad304_02046 pLX_304 0% 86.4% 91.4% V5 (many diffs) n/a
3 TRCN0000478746 CCGAATCTTCATATAACAGCATCT pLX_317 28.7% 86.4% 91.4% V5 (many diffs) n/a
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