Construct: ORF TRCN0000478750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013463.2_s317c1
- Derived from:
- ccsbBroadEn_12632
- DNA Barcode:
- AGGCGAGTGGAGCTCAATCTTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFSD13A (79847)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79847 | MFSD13A | major facilitator superfami... | XM_024448173.1 | 99.8% | 99.5% | 653T>C |
| 2 | human | 79847 | MFSD13A | major facilitator superfami... | XM_011540167.2 | 67.1% | 66.9% | 1_360del;1013T>C |
| 3 | human | 79847 | MFSD13A | major facilitator superfami... | XM_024448171.1 | 67.1% | 66.9% | 1_360del;1013T>C |
| 4 | human | 79847 | MFSD13A | major facilitator superfami... | NM_024789.4 | 47.5% | 47.3% | 1_813del;1466T>C |
| 5 | human | 79847 | MFSD13A | major facilitator superfami... | XM_006717973.3 | 47.5% | 47.3% | 1_813del;1466T>C |
| 6 | human | 79847 | MFSD13A | major facilitator superfami... | XM_006717974.3 | 47.5% | 47.3% | 1_813del;1466T>C |
| 7 | human | 79847 | MFSD13A | major facilitator superfami... | XM_011540160.2 | 47.5% | 47.3% | 1_813del;1466T>C |
| 8 | human | 79847 | MFSD13A | major facilitator superfami... | XM_011540163.2 | 47.5% | 47.3% | 1_813del;1466T>C |
| 9 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016654.1 | 47.5% | 47.3% | 1_813del;1466T>C |
| 10 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016655.1 | 47.5% | 47.3% | 1_813del;1466T>C |
| 11 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016656.1 | 47.5% | 47.3% | 1_813del;1466T>C |
| 12 | human | 79847 | MFSD13A | major facilitator superfami... | XM_011540169.3 | 47.4% | 43.4% | (many diffs) |
| 13 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016657.1 | 33.5% | 30.9% | (many diffs) |
| 14 | human | 79847 | MFSD13A | major facilitator superfami... | XM_017016658.2 | 33.5% | 30.9% | (many diffs) |
| 15 | mouse | 75146 | Mfsd13a | major facilitator superfami... | NM_029186.2 | 41.9% | 43.3% | (many diffs) |
| 16 | mouse | 75146 | Mfsd13a | major facilitator superfami... | XR_386522.1 | 21.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 804
- ORF length:
- 738
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cctggtgcag gtcttccact gccacttcaa cagcaacttc ttccctctct 121 tcctggagca tctgttgtcc gaccatatct ccctttccac gggctccatc ctgttgggcc 181 tctcctatgt cgctccccat ctcaacaacc tctacttcct gtccctgtgc cggcgctggg 241 gcgtctacgc ggtggtgcgg gggctcttcc tgctcaagct gggacttagc ctgctcatgt 301 tgttggccgg cccggaccac ctcagcctgc tgtgcctctt cattgccagc aaccgcgtcT 361 TCACTGAGGG CACCTGTAAG CTGCTGACCT TGGTGGTCAC TGACCTGGTA GACGAGGACC 421 TGGTGCTGAA CCACCGCAAG CAGGCAGCCT CGGCACTCCT CTTTGGCATG GTTGCCTTGG 481 TGACCAAGCC AGGCCAGACC TTTGCCCCGC TGCTGGGCAC CTGGCTGCTC TGTTTCTACA 541 CAGGTCATGA CCTCTTCCAG CAGTCCCTCA TAACCCCTGT GGGGAGTGCC CATCCCTGGC 601 CAGAGCCCCC AGCTCCAGCC CCTGCACAGG CCCCGACGCT CCGCCAGGGC TGCTTCTACC 661 TGCTGGTGCT GGTGCCCATC ACCTGTGCTC TGCTGCAGCT CTTCACCTGG TCCCAGTCCA 721 CGCTGCATGG GAGACGCCTG CACATGGTCA AGGCCCAGCG CCAGAACCTG TCACAGGCCC 781 AAACCCTGGA TGTTAAGATG GTGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 841 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 901 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GGCGAGTGGA 961 GCTCAATCTT CATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1021 att