Construct: ORF TRCN0000478753
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010393.1_s317c1
- Derived from:
- ccsbBroadEn_13284
- DNA Barcode:
- CCTGCACTCTGCGGAGAACTGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ROPN1B (152015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478753
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152015 | ROPN1B | rhophilin associated tail p... | XM_005247138.3 | 100% | 100% | |
| 2 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_001317775.1 | 96.6% | 95% | (many diffs) |
| 3 | human | 152015 | ROPN1B | rhophilin associated tail p... | NM_001012337.3 | 56.6% | 56.6% | 1_276del |
| 4 | human | 152015 | ROPN1B | rhophilin associated tail p... | NM_001308313.2 | 56.6% | 56.6% | 1_276del |
| 5 | human | 152015 | ROPN1B | rhophilin associated tail p... | XM_006713513.3 | 56.6% | 56.6% | 1_276del |
| 6 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_001317774.1 | 54.7% | 53.7% | (many diffs) |
| 7 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_017578.4 | 54.7% | 53.7% | (many diffs) |
| 8 | human | 54763 | ROPN1 | rhophilin associated tail p... | XM_011512933.2 | 54.7% | 53.7% | (many diffs) |
| 9 | human | 54763 | ROPN1 | rhophilin associated tail p... | XM_011512934.2 | 54.7% | 53.7% | (many diffs) |
| 10 | mouse | 76378 | Ropn1 | ropporin, rhophilin associa... | NM_030744.2 | 49.6% | 51.8% | (many diffs) |
| 11 | mouse | 76378 | Ropn1 | ropporin, rhophilin associa... | XM_006522698.3 | 49.6% | 51.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 450
- ORF length:
- 360
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTGGAAAGT GGTGAATCTC CCAACAGATC 121 TGTTTAATAG TGTGATGAAT GTGGGTCGCT TCACGGAGGA GATCGAGTGG CTGAAGTTTT 181 TAGCCCTTGC TTGCAGCGCT CTGGGAGTTA CTATTACCAA AACTCTCAAG ATAGTGTGTG 241 AGGTCTTATC ATGTGACCAC AATGGTGGGT TGCCCCGAAT CCCATTCAGC ACCTTCCAGT 301 TTCTCTACAC GTATATTGCC GAAGTGGATG GGGAGATCTG TGCATCACAT GTCAGCAGGA 361 TGCTAAACTA CATTGAACAG GAAGTAATTG GTCCTGATGG TTTAATCACG GTGAATGACT 421 TTACCCAAAA CCCCAGGGTT TGGCTGGAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 481 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 541 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCTGC 601 ACTCTGCGGA GAACTGCCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 661 tgaaagatt