Construct: ORF TRCN0000478769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016390.2_s317c1
- Derived from:
- ccsbBroadEn_11439
- DNA Barcode:
- CCTGCACGGCTATTCGTGCAAAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR1H4 (9971)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478769
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | NM_001206977.2 | 100% | 100% | |
| 2 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | NM_001206979.2 | 100% | 100% | |
| 3 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | XM_011539040.2 | 100% | 100% | |
| 4 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | NM_005123.4 | 99.1% | 99.1% | 586_587insGTATGTATACAG |
| 5 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | NM_001206993.2 | 95.4% | 93.6% | (many diffs) |
| 6 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | NM_001206992.2 | 94.6% | 92.7% | (many diffs) |
| 7 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | NM_001206978.2 | 89.2% | 89.2% | 443_444ins153 |
| 8 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | XM_011539041.2 | 85% | 83.1% | (many diffs) |
| 9 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | XM_006719719.2 | 53.6% | 47.4% | (many diffs) |
| 10 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | XM_011539042.1 | 52.8% | 46.6% | (many diffs) |
| 11 | human | 9971 | NR1H4 | nuclear receptor subfamily ... | NR_135146.2 | 51.6% | (many diffs) | |
| 12 | mouse | 20186 | Nr1h4 | nuclear receptor subfamily ... | NM_001163504.1 | 85.2% | 92.2% | (many diffs) |
| 13 | mouse | 20186 | Nr1h4 | nuclear receptor subfamily ... | XM_006513391.3 | 85.2% | 92.2% | (many diffs) |
| 14 | mouse | 20186 | Nr1h4 | nuclear receptor subfamily ... | XM_006513393.3 | 84.4% | 91.4% | (many diffs) |
| 15 | mouse | 20186 | Nr1h4 | nuclear receptor subfamily ... | NM_001163700.1 | 81.8% | 87.3% | (many diffs) |
| 16 | mouse | 20186 | Nr1h4 | nuclear receptor subfamily ... | NM_009108.2 | 81.1% | 86.5% | (many diffs) |
| 17 | mouse | 20186 | Nr1h4 | nuclear receptor subfamily ... | XR_001779493.1 | 77.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1497
- ORF length:
- 1428
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggatcaaaa atgaatctca ttgaacattc ccatttacct accacagatg 121 aattttcttt ttctgaaaat ttatttggtg ttttaacaga acaagtggca ggtcctctgg 181 gacagaacct ggaagtggaa ccatactcgc aatacagcaa tgttcagttt ccccaagttc 241 aaccacagat ttcctcgtca tcctattatt ccaacctggg tttctacccc cagcagcctg 301 aagagtggta ctctcctgga atatatgaac tcaggcgtat gccagctgag actctctacc 361 agggagaaac tgaggtagca gagatgcctg taacaaagaa gccccgcatg ggcgcgtcag 421 cagggaggat caaaggggat gagctgtgtg ttgtttgtgg agacagagcc tctggatacc 481 actataatgc actgacctgt gaggggtgta aaggtttctt caggagaagc attaccaaaa 541 acgctgtgta caagtgtaaa aacgggggca actgtgtgat ggatatgtac atgcgaagaa 601 agtgtcaaga gtgtcgacta aggaaatgca aagagatggg aatgttggct gaatgtatgt 661 atacaggctt gttaactgaa attcagtgta aatctaagcg actgagaaaa aatgtgaagc 721 agcatgcaga tcagaccgtg aatgaagaca gtgaaggtcg tgacttgcga caagtgacct 781 cgacaacaaa gtcatgcagg gagaaaactg aactcacccc agatcaacag actcttctac 841 attttattat ggattcatat aacaaacaga ggatgcctca ggaaataaca aataaaattt 901 taaaagaaga attcagtgca gaagaaaatt ttctcatttt gacggaaatg gcaaccaatc 961 atgtacaggt tcttgtagaa ttcacaaaaa agctaccagg atttcagact ttggaccatg 1021 aagaccagat tgctttgctg aaagggtctg cggttgaagc tatgttcctt cgttcagctg 1081 agattttcaa taagaaactt ccgtctgggc attctgaccT ATTGGAAGAA AGAATTCGAA 1141 ATAGTGGTAT CTCTGATGAA TATATAACAC CTATGTTTAG TTTTTATAAA AGTATTGGGG 1201 AACTGAAAAT GACTCAAGAG GAGTATGCTC TGCTTACAGC AATTGTTATC CTGTCTCCAG 1261 ATAGACAATA CATAAAGGAT AGAGAGGCAG TAGAGAAGCT TCAGGAGCCA CTTCTTGATG 1321 TGCTACAAAA GTTGTGTAAG ATTCACCAGC CTGAAAATCC TCAACACTTT GCCTGTCTCC 1381 TGGGTCGCCT GACTGAATTA CGGACATTCA ATCATCACCA CGCTGAGATG CTGATGTCAT 1441 GGAGAGTAAA CGACCACAAG TTTACCCCAC TTCTCTGTGA AATCTGGGAC GTGCAGTTGC 1501 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1561 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1621 ATATATCTTG TGGAAAGGAC GACCTGCACG GCTATTCGTG CAAAGCACGC GTTAAGTCga 1681 caatcaacct ctggattaca aaatttgtga aagatt