Construct: ORF TRCN0000478779
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008230.1_s317c1
- Derived from:
- ccsbBroadEn_10871
- DNA Barcode:
- TCTCAGACAGATCTATGCCGGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HELLS (3070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478779
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289075.2 | 80.5% | 80.5% | 1_252del |
| 2 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289074.2 | 72.9% | 72.9% | 1_387del |
| 3 | human | 3070 | HELLS | helicase, lymphoid specific | XM_024447967.1 | 56.4% | 56.4% | 1_804del |
| 4 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289073.2 | 49.7% | 49.7% | 1_1056del |
| 5 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289072.2 | 49.1% | 49.1% | 1_1080del |
| 6 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289071.2 | 48.7% | 48.7% | 1_1098del |
| 7 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289070.1 | 47% | 47% | 1_1176del |
| 8 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289069.2 | 43.1% | 43.1% | 1_1374del |
| 9 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289068.2 | 42.3% | 42.3% | 1_1422del |
| 10 | human | 3070 | HELLS | helicase, lymphoid specific | NM_018063.5 | 41.5% | 41.5% | 1_1470del |
| 11 | human | 3070 | HELLS | helicase, lymphoid specific | NM_001289067.1 | 39.3% | 39.3% | 1_1608del |
| 12 | human | 3070 | HELLS | helicase, lymphoid specific | XM_024447968.1 | 39.3% | 39.3% | 1_1608del |
| 13 | mouse | 15201 | Hells | helicase, lymphoid specific | XM_006526700.3 | 65.2% | 66.6% | (many diffs) |
| 14 | mouse | 15201 | Hells | helicase, lymphoid specific | NM_008234.3 | 37.9% | 38.7% | (many diffs) |
| 15 | mouse | 15201 | Hells | helicase, lymphoid specific | XM_006526699.3 | 37.9% | 38.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1110
- ORF length:
- 1044
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tggatccagt gagaaagaaa caattgagtt aagtcctact ggtcgaccaa 121 aacgacgaac tagaaaatca ataaattaca gcaaaataga tgatttccct aatgaattgg 181 aaaaactgat cagtcaaata cagccagagg tggaccgaga aagagctgtt gtggaagtga 241 atatccctgt agaatctgaa gttaatctga agctgcagaa tataatgatg ctacttcgta 301 aatgttgtaa tcatccatat ttgattgaat atcctataga ccctgttaca caagaattta 361 agatcgatga agaattggta acaaattctg ggaagttctt gattttggat cgaatgctgc 421 cagaactaaa aaaaagaggt cacaaggtgc tgcttttttc acaaatgaca agcatgttgg 481 acattttgat ggattactgc catctcagag atttcaactt cagcaggctt gatgggtcca 541 tgtcttactc agagagagaa aaaaacatgc acagcttcaa cacggatcca gaggtgttta 601 tcttcttagt gagtacacga gctggtggcc tgggcattaa tctgactgca gcagatacag 661 ttatcattta tgatagtgat tggaaccccc agtcggatct tcaggcccag gatagatgtc 721 atagaattgg tcagacaaag ccagttgttg tttatcgcct tgttacagca aatactatcg 781 atcagaaaat tgtggaaaga gcagctgcta aaaggaaact ggaaaagttg aTCATCCATA 841 AAAATCATTT CAAAGGTGGT CAGTCTGGAT TAAATCTGTC TAAGAATTTC TTAGATCCTA 901 AGGAATTAAT GGAATTATTA AAATCTAGAG ATTATGAAAG GGAAATAAAA GGATCAAGAG 961 AGAAGGTCAT TAGTGATAAA GATCTAGAGT TGTTGTTAGA TCGAAGTGAT CTTATTGATC 1021 AAATGAATGC TTCAGGACCA ATTAAAGAGA AGATGGGGAT ATTCAAGATA TTAGAAAATT 1081 CTGAAGATTC CAGTCCTGAA TGTTTGTTTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCTCA 1261 GACAGATCTA TGCCGGTGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt