Construct: ORF TRCN0000478791
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013605.1_s317c1
- Derived from:
- ccsbBroadEn_05102
- DNA Barcode:
- GTTGATACAACTCGAAATACACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM122C (159091)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478791
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 159091 | FAM122C | family with sequence simila... | NM_138819.4 | 100% | 100% | |
| 2 | human | 159091 | FAM122C | family with sequence simila... | XM_005262384.4 | 80.8% | 80.8% | 1_108del |
| 3 | human | 159091 | FAM122C | family with sequence simila... | NM_001365745.1 | 79.6% | 79.6% | 344_345ins93 |
| 4 | human | 159091 | FAM122C | family with sequence simila... | XM_011531298.3 | 78.5% | 76.9% | (many diffs) |
| 5 | human | 159091 | FAM122C | family with sequence simila... | XM_017029308.1 | 78.5% | 76.9% | (many diffs) |
| 6 | human | 159091 | FAM122C | family with sequence simila... | NM_001365748.1 | 70% | 67.7% | 439_527del;546_651del |
| 7 | human | 159091 | FAM122C | family with sequence simila... | NM_001170779.3 | 69.9% | 62.9% | (many diffs) |
| 8 | human | 159091 | FAM122C | family with sequence simila... | XM_005262386.3 | 64.3% | 64.3% | 1_108del;452_453ins93 |
| 9 | human | 159091 | FAM122C | family with sequence simila... | XM_024452343.1 | 60.3% | 58.3% | 1_108del;547_632del;651_756del |
| 10 | human | 159091 | FAM122C | family with sequence simila... | XM_011531297.2 | 60% | 58.1% | 1_108del;547_635del;654_759del |
| 11 | human | 159091 | FAM122C | family with sequence simila... | XM_005262383.2 | 59.3% | 53.2% | (many diffs) |
| 12 | human | 159091 | FAM122C | family with sequence simila... | XM_006724735.1 | 58% | 55.8% | 1_108del;547_643del;662_786del |
| 13 | human | 159091 | FAM122C | family with sequence simila... | NM_001365747.1 | 57.7% | 55.1% | (many diffs) |
| 14 | human | 159091 | FAM122C | family with sequence simila... | XM_006724734.4 | 55.5% | 54.4% | (many diffs) |
| 15 | human | 159091 | FAM122C | family with sequence simila... | XM_011531296.2 | 53.5% | 52.5% | (many diffs) |
| 16 | human | 159091 | FAM122C | family with sequence simila... | NM_001170782.2 | 52.2% | 39.1% | (many diffs) |
| 17 | human | 159091 | FAM122C | family with sequence simila... | XM_011531305.2 | 50% | 44.9% | (many diffs) |
| 18 | human | 159091 | FAM122C | family with sequence simila... | XM_011531295.1 | 49.3% | 48.1% | (many diffs) |
| 19 | human | 159091 | FAM122C | family with sequence simila... | NM_001170783.2 | 49.3% | 44% | (many diffs) |
| 20 | human | 159091 | FAM122C | family with sequence simila... | NM_001170784.2 | 49.3% | 44% | (many diffs) |
| 21 | human | 159091 | FAM122C | family with sequence simila... | XM_005262382.1 | 49% | 48% | (many diffs) |
| 22 | human | 159091 | FAM122C | family with sequence simila... | NM_001365744.1 | 46.9% | 44.7% | (many diffs) |
| 23 | human | 159091 | FAM122C | family with sequence simila... | XM_011531306.2 | 45.7% | 43.6% | (many diffs) |
| 24 | human | 159091 | FAM122C | family with sequence simila... | NM_001170780.1 | 39.9% | 35.6% | (many diffs) |
| 25 | human | 159091 | FAM122C | family with sequence simila... | NR_158603.1 | 33.8% | (many diffs) | |
| 26 | human | 159091 | FAM122C | family with sequence simila... | XM_017029309.1 | 29.6% | 27.3% | 0_1ins255;184_280del;299_423del |
| 27 | human | 159091 | FAM122C | family with sequence simila... | XM_011531299.1 | 24.1% | 22.9% | (many diffs) |
| 28 | human | 159091 | FAM122C | family with sequence simila... | XM_011531301.2 | 24.1% | 22.9% | (many diffs) |
| 29 | human | 159091 | FAM122C | family with sequence simila... | XM_011531302.2 | 24.1% | 22.9% | (many diffs) |
| 30 | human | 159091 | FAM122C | family with sequence simila... | NR_158606.1 | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc acaggagaaa atgaaactag gtttcaagtc gctgccgagt tccactaccg 121 cagacggcaa cattcTGAGA AGAGTCAACA GTGCCCCTTT GATCAATGGA CTTGGTTTTA 181 ATTCACAGGT GTTGCAAGCT GACATGTTAA GAATTAGGAC AAACAGAACA ACATTTAGGA 241 ATCGACGCTC TCTGTTGTTG CCACCTCCTC CCTTTCATGG TTCCATCAGC CGCCTTCATC 301 AAATCAAACA GGAAGAAGCC ATGGATTTAA TAAATAGAGA AACAATGTCT GAATGGAAGC 361 TACAAAGTGA GATACAGATA AGTCACTCTT GGGAAGAAGG CTTGAAACTG AATGACAATG 421 GCTTACAGAA ATCATCCTCT CTAAAGTGCA TTGATTTAAC TCCAGTATCC TCAATGGCTT 481 CTTCCATCAA GAAGACTGGG AAGTCTACTT CCAGCTACTT TTGCCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGAGTT GATACAACTC GAAATACACC TACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t