Construct: ORF TRCN0000478836
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017992.2_s317c1
- Derived from:
- ccsbBroadEn_00302
- DNA Barcode:
- AGGGATCATCGGATTTCCATGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FOXN3 (1112)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478836
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1112 | FOXN3 | forkhead box N3 | NM_005197.4 | 100% | 100% | |
| 2 | human | 1112 | FOXN3 | forkhead box N3 | NM_001085471.2 | 95.5% | 95.3% | 746_811del |
| 3 | mouse | 71375 | Foxn3 | forkhead box N3 | NM_183186.2 | 89.8% | 93.1% | (many diffs) |
| 4 | mouse | 71375 | Foxn3 | forkhead box N3 | XM_006516239.3 | 89.8% | 93.1% | (many diffs) |
| 5 | mouse | 71375 | Foxn3 | forkhead box N3 | XM_006516240.2 | 89.8% | 93.1% | (many diffs) |
| 6 | mouse | 71375 | Foxn3 | forkhead box N3 | XM_017315205.1 | 85.9% | 89.1% | (many diffs) |
| 7 | mouse | 71375 | Foxn3 | forkhead box N3 | XM_006516232.3 | 85.7% | 88.9% | (many diffs) |
| 8 | mouse | 71375 | Foxn3 | forkhead box N3 | XM_006516233.2 | 85.7% | 88.9% | (many diffs) |
| 9 | mouse | 71375 | Foxn3 | forkhead box N3 | XM_006516234.2 | 85.7% | 88.9% | (many diffs) |
| 10 | mouse | 71375 | Foxn3 | forkhead box N3 | XM_006516236.3 | 85.7% | 88.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1470
- ORF length:
- 1404
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tccagtcatg cctcccagta agaagccaga aagctcagga attagtgtct 121 ccagtggact gagtcagtgt tacgggggca gcggtttctc caaggccctt caggaagacg 181 atgacctcga cttttctctg cctgacatcc gattagaaga gggggccatg gaagatgaag 241 agctgaccaa cctgaactgg ctgcacgaga gcaagaactt gctgaagagc tttggggagt 301 cggtcctcag gagtgtcagc cccgtccagg acctggacga tgacaccccc ccatcccctg 361 cccactctga catgccctac gatgccaggc agaaccccaa ctgcaaaccc ccctactcct 421 tcagctgcct catatttatg gccatcgagg actctccaac caagcgcctg ccagtgaagg 481 atatctacaa ctggatcttg gaacattttc cgtattttgc aaatgcacct actgggtgga 541 aaaactcagt gagacacaat ttatcattga ataagtgttt taagaaagtg gacaaagaga 601 ggagtcagag tattgggaaa gggtcgttgt ggtgcataga cccagagtat agacaaaatc 661 taattcaggc tttgaaaaag acaccttatc acccacaccc acacgtgttc aatacacctc 721 ccacctgtcc tcaggcatat caaagcacat caggtccacc catctggccg ggcagtacct 781 tcttcaagag aaatggagcc cttctccaag ttcctccagg agtgatccaa aatggagcgc 841 gggtcctgag ccgagggctg tttcctggcg tgcggccgct gccaatcact cccattgggg 901 tgacagcggc catgaggaat ggcatcacca gctgccggat gcggactgag agtgagccat 961 cttgtggctc cccagtggtc agcggagacc ccaaggagga tcacaactac agcagtgcca 1021 agtcctccaa cgcccggagc acctcgccca ccagcgactc catctcctcc tcctcctcct 1081 cagccgacga ccactatgag tttgccacca agggGAGCCA GGAGGGCAGC GAGGGCAGCG 1141 AGGGGAGCTT CCGGAGCCAC GAGAGCCCCA GCGACACGGA AGAGGACGAC AGGAAGCACA 1201 GCCAGAAGGA GCCCAAGGAT TCTCTGGGGG ACAGCGGGTA CGCATCCCAG CACAAGAAGC 1261 GCCAGCACTT CGCCAAGGCC AGGAAGGTCC CCAGCGACAC ACTGCCCCTC AAAAAGAGAC 1321 GCACCGAAAA GCCCCCCGAG AGCGATGATG AGGAGATGAA AGAAGCGGCA GGGTCCCTCC 1381 TGCACTTAGC AGGGATCCGG TCCTGTTTGA ATAACATCAC CAATCGGACG GCAAAGGGGC 1441 AGAAAGAGCA AAAGGAAACC ACAAAAAATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGGGA 1621 TCATCGGATT TCCATGCCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt