Construct: ORF TRCN0000478870
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012484.1_s317c1
- Derived from:
- ccsbBroadEn_12956
- DNA Barcode:
- TAAAAATTTCCGGTACATCCCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGFN1 (91156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478870
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91156 | IGFN1 | immunoglobulin like and fib... | NM_001367841.1 | 68.1% | 67.6% | (many diffs) |
| 2 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_017002787.1 | 23.9% | 23.9% | (many diffs) |
| 3 | human | 91156 | IGFN1 | immunoglobulin like and fib... | NM_001164586.2 | 23.3% | 23.3% | (many diffs) |
| 4 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_011510120.3 | 23.3% | 23.3% | (many diffs) |
| 5 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_011510121.3 | 23.3% | 23.3% | (many diffs) |
| 6 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_005245580.3 | 22.7% | 22.7% | (many diffs) |
| 7 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XR_921994.2 | 19.7% | (many diffs) | |
| 8 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_006711618.3 | 16.3% | 15.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2673
- ORF length:
- 2604
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggtggcag cctatgggag agaactgggg gtgcctggag gagatgctga 121 atgaagatca gagccgggag ccccctggtc accttggtag caggagaagt ggcaaagacg 181 gcaggttgga catctatgga gagaggagag atgctacccg gagttccaca tccagataca 241 agcctggcac tggcagtttc tccaaggatg cccaaggccc catgggccac ttctcccagg 301 gcctggctga catggaagtg cagccggggg aggccgccac actctcctgt accctcacca 361 gtgacctggg acctggcacc tggtttaagg atggcgtcaa gctcaccacc caggatggag 421 tcatctttaa gcaagacggt ctcgtgcaca gcctcttcat cacgcatgtg caggggaccc 481 aagctgggag gtacaccttt gtagctggtg accagcagag cgaggccacc ctgaccgtcc 541 aggattcccc taccattgct ccagatgtga cagagaaact gagagagcca ctggtggtca 601 aggctgggaa gccggtgata gtgaagatcc ccttccagag ccacctcccc attcaggctg 661 cctggaggaa ggacggggct gaggtggtgg gcagcagtga cagggaggcc caggtggacc 721 tgggggatgg ctacacgcgg ctgtgcctcc ccagcgcagg caggaaggac tgtggccagt 781 acagcgtgac actgaggagt gagggaggct ctgtgcaggc cgagctcact ctgcaagtca 841 tagacaagcc tgatccccca caaggcccca tggaggttca ggattgccat agggctggcg 901 tctgcctccg ctggcggccc ccaagggaca atgggggccg gactgtagag tgctacgtgg 961 tggagagacg gcaggctggc aggagcactt ggctgaaggt gggcgaggcc cccgctgaca 1021 gcaccacctt cacggatgcc catgtggagc caggcaggaa gtataccttc cgagtgcggg 1081 ctgtgacctc agagggggct ggcgaggccc tggagtctga ggagatattg gtggctcctg 1141 aggctctccc caaggcccct tccgcgccag ccatcctgtc ggcctccagc cagggcatca 1201 cactgacatg gacagcacct cggggccccg gcagcgccca catcctgggc tacctgatcg 1261 agaggcgtaa gaaggggagc aacacctgga cggcagtgaa cgaccagccg gtgcctgaga 1321 ggaggtggac ggtggcggac gtgcggcagg gctgtcagta tgagttccgg gtcacagctg 1381 tggctccctc aggtcccgga gagcctggac ctccatcgga tgccgtcttt gctcgggacc 1441 ccatgagacc ccctgggctg gtgaggaatc tccaagtcac agacagatcg aacaccagca 1501 tcactctgag ctgggctggg ccagacaccc aggaagggga tgaagcccag gggtatgtgg 1561 tggagctgtg cagctcagac agtctccagt ggctcccgtg ccatgtaggc accgtgccag 1621 tcaccaccta cacggccaag gggcttcggc ctggagaggg ctacttcgtg cgggtgacag 1681 cagttaatga aggaggccag agccagccca gtgccctgga cacattagtg caagccatgc 1741 ctgttactgt ctgtcccaag ttcctcgtgg actccagcac caaggacttg ctgacagtca 1801 aggtcgggga cacagttcgt gtgcccgtct cctttgaagc catgcccatg cctgaggtga 1861 cctggctgaa ggatggcttg cccttgccca aaagaagtgt gactgtcact aaggatggcc 1921 tcacccagct tctgatccct gtggctggac tctcagacag tggtctctac actgtggtgc 1981 tgaggaccct gcaggggaag gaggttgccc acagcttccg tatcagggtg gcagcatgcc 2041 cgcaggcccc tgggcccatc cacctgcagg agaacgtgcc tgggacggtg acggccgagt 2101 gggaaccctc tcctgacgag gcccaggatg tcccgctgca ctacgcggtg ttcacacgct 2161 cctcagcgca cggtccctgg cacgaggcag ccgaccgcat ctacaccaac cgcttcaccc 2221 tcctgggcat cctccccggc cacgaatacc acttcagggt ggtggccaag aatgagctgg 2281 gggccagcaa accctcggac accagccagc cctggtgcat cccccggcag cgcgacaggt 2341 tcacagtgaa ggctccgtgc taccgggagc ccgacctgag ccagaagccc cggttccTGG 2401 TGGGCCTGCG GTCCCACCTG CTGCCCCAGG GCTGCGAGTG CTGCATGAGC TGTGCCGTGC 2461 AGGGCTCGCC CCGGCCCCAC GTCACCTGGT TCAAGAATGA CCGCAGCCTG GAAGGAAACC 2521 CCGCGGTGTA CAGCACTGAC CTGCTGGGCG TGTGCTCCCT CACCATCCCC AGCGTATCCC 2581 CGAAGGACAG CGGGGAGTAC AAGGCTGTGG CTGAGAACAC GCTGGGCCAG GCAGTCAGCA 2641 CTGCCACCCT CATTGTCATA GAACCCAGCA CCTTGCCAAC TTTCTTGTAC AAAGTGGTTG 2701 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2761 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 2821 AAAATTTCCG GTACATCCCC CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2881 ttgtgaaaga tt