Construct: ORF TRCN0000478917
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011654.1_s317c1
- Derived from:
- ccsbBroadEn_08371
- DNA Barcode:
- ATAACAACAGCAGAAGTCTCTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NLGN3 (54413)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478917
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54413 | NLGN3 | neuroligin 3 | NM_018977.4 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 54413 | NLGN3 | neuroligin 3 | NM_181303.2 | 97.3% | 97.1% | (many diffs) |
| 3 | human | 54413 | NLGN3 | neuroligin 3 | NM_001166660.1 | 97.3% | 97.1% | (many diffs) |
| 4 | human | 54413 | NLGN3 | neuroligin 3 | XM_006724662.4 | 88.3% | 86.4% | (many diffs) |
| 5 | human | 54413 | NLGN3 | neuroligin 3 | NM_001321276.2 | 85.6% | 85.5% | (many diffs) |
| 6 | human | 54413 | NLGN3 | neuroligin 3 | XM_006724663.4 | 83.6% | 83.4% | (many diffs) |
| 7 | human | 54413 | NLGN3 | neuroligin 3 | XM_011530974.3 | 83.6% | 83.4% | (many diffs) |
| 8 | human | 54413 | NLGN3 | neuroligin 3 | XM_017029597.2 | 65.2% | 64.5% | (many diffs) |
| 9 | mouse | 245537 | Nlgn3 | neuroligin 3 | NM_172932.4 | 92.7% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2550
- ORF length:
- 2484
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gctgcggctt ggcccgccct cgctgtccct gagccccaag cccacggttg 121 gcaggagcct gtgcctcacc ctgtggttcc tcagtttggc gctgagggcc agtacccagg 181 ccccagcacc cacagtcaac actcactttg ggaagctaag gggtgcccga gtaccactgc 241 ccagtgagat cctggggcct gtggaccaat acctgggggt gccctacgca gctcccccga 301 tcggcgagaa acgtttcctg ccccctgaac cacccccata ctggtcgggc atccggaacg 361 ccacacactt tccaccagtg tgcccccaga acatccacac agctgtgccc gaagtcatgc 421 tgccggtctg gttcactgcc aacttggata tcgtcgctac ttacatccag gagcccaacg 481 aagactgtct ctacctgaac gtctatgtgc cgacggagga tggatccggc gctaagaaac 541 agggcgagga cttagcggat aatgacgggg atgaagatga agacatccgg gacagtggtg 601 ctaaacccgt catggtctac atccacggag gctcttacat ggaagggaca ggcaacatga 661 ttgatggcag catcctcgcc agttatggca atgtcatcgt catcaccctc aactatcggg 721 ttggagtgct aggtttcctg agtactggag atcaggctgc caagggcaac tatgggctcc 781 ttgaccagat ccaggccctc cgctgggtga gcgagaatat tgccttcttc gggggagacc 841 cccgccggat cactgtcttt ggctcgggca ttggtgcatc ctgcgtcagc ctcctaacgt 901 tgtcacatca ctcagaggga cttttccaga gagccatcat ccaaagtggc tctgctctgt 961 ccagctgggc tgtgaactac caaccagtga agtacaccag cctgctggca gacaaagtgg 1021 gctgtaatgt gctggacacc gtggatatgg tggactgtct tcggcaaaag agtgccaagg 1081 agctggtaga gcaggacatc cagccagccc gctaccacgt ggcctttggc cctgtgattg 1141 atggtgatgt cattcctgat gaccctgaga tcctcatgga gcagggcgag ttcctcaact 1201 atgacatcat gctaggtgtc aaccagggcg agggtctcaa gtttgtggaa ggggtggtgg 1261 accctgagga tggtgtctct ggcactgact ttgactattc cgtctccaat tttgtggaca 1321 atctgtatgg ctatcctgag ggtaaggaca ccctgcgaga gaccatcaag ttcatgtata 1381 cagactgggc agaccgtgac aaccctgaga cccgccgtaa aacactggtg gcactcttca 1441 ctgaccacca gtgggtggag ccctcagtgg tgacagccga tctgcatgcc cgctacggct 1501 cgcctaccta cttctacgcc ttctatcatc actgccagag cctcatgaag cctgcttggt 1561 cagatgcagc tcatggggat gaagtaccct atgtttttgg ggttcctatg gtaggcccca 1621 ctgacctttt cccctgcaac ttctccaaga atgatgttat gctcagtgct gtcgtcatga 1681 cctattggac caactttgcc aagactgggg atcccaacaa gccggtcccc caggacacca 1741 agttcattca caccaaggcc aaccgctttg aggaagtggc ctggtccaaa tacaatcccc 1801 gagaccagct ctaccttcac atcgggctga aaccaagggt ccgagatcat taccgggcca 1861 ctaaggtggc cttttggaaa catctggtgc cgcacctata caacctgcat gacatgttcc 1921 actatacgtc caccaccacc aaagtgccgc ctccggatac cacccacagc tcccacatca 1981 cccgcaggcc caatggcaag acctggagca ccaagcggcc agccatctca cctgcctaca 2041 gcaacgagaa tgcccagggg tcctggaacg gggaccagga tgcagggcca ctcctggtgg 2101 agaaccctcg tgactactcc actgaattaa gtgtcaccat cgccgtgggg gcctccatcc 2161 tgttccttaa cgttctggcc ttcgctgccc tctactaccg taaggacaaa cggcgccagg 2221 agcccctgcg gcagcctagc cctcagcggG GAGCCTGGGC CCCGGAGTTG GGAGCTGCTC 2281 CAGAGGAGGA GCTGGCAGCA TTACAACTGG GCCCCACCCA CCACGAGTGT GAGGCCAGTC 2341 CCCCCCATGA CACGCTGCGC CTCACTGCAT TGCCCGACTA CACCCTGACC CTGCGGCGCT 2401 CCCCGGATGA CATCCCACTC ATGACCCCCA ACACCATCAC TATGATCCCC AACTCCCTGG 2461 TAGGGCTGCA GACATTGCAC CCCTATAACA CCTTTGCCGC AGGGTTCAAC AGTACCGGGC 2521 TGCCCCACTC ACACTCCACT ACCCGGGTAT ACCCAACTTT CTTGTACAAA GTGGTTGATA 2581 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2641 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATAAC 2701 AACAGCAGAA GTCTCTGCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2761 tgaaagatt