Construct: ORF TRCN0000478933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000445.1_s317c1
- Derived from:
- ccsbBroadEn_09423
- DNA Barcode:
- TTTATCGGTGCGTTTGGGGAAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB3C (115827)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478933
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115827 | RAB3C | RAB3C, member RAS oncogene ... | NM_138453.4 | 100% | 100% | |
| 2 | human | 115827 | RAB3C | RAB3C, member RAS oncogene ... | NM_001317915.2 | 96.2% | 96.9% | 1_10delATGGAGCTGT;13_14insAGACAC;16_17insGCGCCCATGC |
| 3 | mouse | 67295 | Rab3c | RAB3C, member RAS oncogene ... | NM_023852.5 | 89.4% | 97.3% | (many diffs) |
| 4 | mouse | 67295 | Rab3c | RAB3C, member RAS oncogene ... | XM_006517741.3 | 86% | 93.8% | (many diffs) |
| 5 | mouse | 67295 | Rab3c | RAB3C, member RAS oncogene ... | XM_006517740.3 | 85.9% | 94.2% | (many diffs) |
| 6 | mouse | 67295 | Rab3c | RAB3C, member RAS oncogene ... | XM_006517742.3 | 67.1% | 71.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag acacgaagcg cccatgcaga tggcctctgc ccaagatgcc aggtacggcc 121 agaaagactc ctctgatcag aactttgact acatgttcaa attactcatc atcggcaata 181 gcagtgtggg gaaaacatct tttctattcc gttatgcaga tgactccttt acatctgcat 241 tcgtcagcac agttgggatc gatttcaaag taaaaactgt attcaaaaat gaaaagagaa 301 tcaagcttca gatttgggac acagcaggcc aggaaagata caggactatc accacagcct 361 attatcgtGG AGCCATGGGC TTTATTTTAA TGTATGACAT TACAAATGAA GAATCCTTCA 421 ATGCAGTACA AGATTGGTCA ACTCAAATCA AAACATACTC TTGGGACAAT GCCCAAGTTA 481 TTCTGGTTGG GAACAAGTGT GACATGGAAG ACGAGCGGGT CATCTCAACT GAGCGAGGTC 541 AACATTTAGG AGAACAGCTT GGGTTTGAGT TTTTTGAAAC AAGTGCCAAG GACAACATTA 601 ATGTCAAGCA GACATTTGAG CGCCTTGTGG ATATCATCTG CGACAAAATG TCAGAGAGTT 661 TGGAGACTGA TCCTGCCATC ACTGCTGCAA AGCAGAACAC GAGACTCAAG GAAACTCCTC 721 CTCCACCGCA GCCCAACTGT GCCTGCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTTATCGG 901 TGCGTTTGGG GAAGTCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt