Construct: ORF TRCN0000478961
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007259.1_s317c1
- Derived from:
- ccsbBroadEn_13940
- DNA Barcode:
- GAAAGCGCCTCCCTATTTAAGAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RBL1 (5933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478961
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5933 | RBL1 | RB transcriptional corepres... | NM_183404.4 | 99.9% | .7% | 8delA |
| 2 | human | 5933 | RBL1 | RB transcriptional corepres... | NM_002895.5 | 94.8% | .7% | (many diffs) |
| 3 | human | 5933 | RBL1 | RB transcriptional corepres... | XM_024451957.1 | 92.4% | .7% | (many diffs) |
| 4 | human | 5933 | RBL1 | RB transcriptional corepres... | XM_024451956.1 | 91.7% | .7% | (many diffs) |
| 5 | human | 5933 | RBL1 | RB transcriptional corepres... | XM_017027992.1 | 85.2% | .8% | (many diffs) |
| 6 | human | 5933 | RBL1 | RB transcriptional corepres... | XM_006723842.3 | 78.8% | .8% | (many diffs) |
| 7 | human | 5933 | RBL1 | RB transcriptional corepres... | XM_024451958.1 | 69.5% | 1% | (many diffs) |
| 8 | human | 5933 | RBL1 | RB transcriptional corepres... | NM_001323281.2 | 54.9% | 1.2% | (many diffs) |
| 9 | human | 5933 | RBL1 | RB transcriptional corepres... | NM_001323282.2 | 54.9% | 1.2% | (many diffs) |
| 10 | mouse | 19650 | Rbl1 | retinoblastoma-like 1 (p107) | NM_011249.2 | 82.7% | .7% | (many diffs) |
| 11 | mouse | 19650 | Rbl1 | retinoblastoma-like 1 (p107) | NM_001139516.1 | 52% | 1.3% | (many diffs) |
| 12 | mouse | 19650 | Rbl1 | retinoblastoma-like 1 (p107) | XM_006499018.1 | 48.1% | 1.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 147
- ORF length:
- 81
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cgggacaagc cccacgctga gggggcggcg gtggtcgccg cagccgggga 121 ggcgctacag gccctgtgcc aggagctgaa cctggacgag gggagcgcgg ccgaagccct 181 ggacgacttt actgccatcc gaggcaacta cagcctagag ggagaagtta cacactggtt 241 ggcatgttca ttatatgttg catgccgcaa aagcattatt cccacggttg gaaagggtat 301 catggaaggc aactgtgttt cacttaccag aatactacgt tcagctaaat taagtttaat 361 acaatttttt agtaaaatga agaaatggat ggacatgtca aatctaccac aagaatttcg 421 tgaacgtata gaaaggctag agagaaattt tgaggtgtct actgtaatat tcaaaaaata 481 tgagccaatt tttttagata tatttcaaaa tccatatgaa gaaccaccaa agttaccacg 541 aagccggaag cagaggagga ttccttgcag tgttaaggat ctgtttaatt tctgttggac 601 actttttgtt tatactaagg gtaattttcg gatgattggg gatgacttag taaactctta 661 tcatttactt ctatgctgct tggatctgat ttttgccaat gcgattatgt gcccaaatag 721 acaagacttg ctaaatccat catttaaagg tttaccatct gattttcata ctgctgactt 781 tacggcttct gaagagccac cctgcatcat tgctgtactg tgtgaactgc atgatggact 841 tctcgtagaa gcaaaaggaa taaaggagca ctactttaag ccatatattt caaaactctt 901 tgacaggaag atattaaaag gagaatgcct cctggacctt tcaagtttta ctgataatag 961 caaagcagtg aataaggagt atgaagagta tgttctaact gttggtgatt ttgatgagag 1021 gatctttttg ggagcagacg cagaagagga aattggaaca cctcgaaagt tcactcgtga 1081 caccccatta gggaaactga cagcacaggc taatgtggag tataaccttc aacagcactt 1141 tgaaaaaaaa aggtcatttg caccttctac cccactgacc ggacggagat atttacgaga 1201 aaaagaagca gtcattactc ctgttgcatc agccacccaa agtgtgagcc ggttacagag 1261 tattgtggct ggtctgaaaa atgcaccaag tgaccaactt ataaatattt ttgaatcttg 1321 tgtgcgtaat cctgtggaaa acattatgaa aatactaaaa ggaataggag agactttctg 1381 tcaacactat actcaatcaa cagatgaaca gccaggatct cacatagact ttgctgtaaa 1441 cagactaaag ctggcagaaa ttttgtatta taaaatacta gagactgtaa tggttcagga 1501 aacacgaaga cttcatggaa tggacatgtc agttctttta gagcaagata tatttcatcg 1561 ttccttgatg gcttgttgtt tggaaattgt gctctttgcc tatagctcac ctcgtacttt 1621 tccttggatt attgaagttc tcaacttgca accattttac ttttataagg ttattgaggt 1681 ggtgatccgc tcagaagagg ggctctcaag ggacatggtg aaacacctaa acagcattga 1741 agaacagatt ttggagagtt tagcatggag tcacgattct gcactgtggg aggctctcca 1801 ggtttctgca aacaaagttc ctacctgtga agaagttata ttcccaaata actttgaaac 1861 aggaaatgga ggaaatgtgc agggacatct tcccctgatg ccaatgtctc ctctaatgca 1921 cccaagagtc aaggaagttc gaactgacag tgggagtctt cgaagagata tgcaaccatt 1981 gtctccaatt tctgtccatg aacgctacag ttctcctacc gcagggagtg ctaagagaag 2041 actctttgga gaggaccccc caaaggaaat gcttatggac aagatcataa cagaaggaac 2101 aaaattgaaa atcgctcctt cttcaagcat tactgctgaa aatgtatcaa ttttacctgg 2161 tcaaactctt ctaacaatgg ccacagcccc agtaacagga acaacaggac ataaagttac 2221 aattccatta catggtgtcg caaatgatgc tggagagatc acactgatac ctctttccat 2281 gaatacaaat caggagtcca aagtcaagag tcctgtatca cttactgctc attcattaat 2341 tggtgcttct ccaaaacaga ccaatctgac taaagcacaa gaggtacatt caactggaat 2401 aaacaggcca aagagaactg ggtccttagc actattttac agaaaggtct atcatttggc 2461 aagtgtacgc ttacgtgatc tatgtctaaa actggatgtt tcaaatgagt tacgaaggaa 2521 gatatggacg tgttttgaat tcactttagt tcactgtcct gatctaatga aagacaggca 2581 tttggatcag ctcctccttt gtgcctttta tatcatggca aaggtaacaa aagaagaaag 2641 aacttttcaa gaaattatga aaagttatag gaatcagccc caagctaata gtcacgtata 2701 tagaagtgtt cTGCTGAAAA GTATTCCAAG AGAAGTTGTG GCATATAATA AAAATATAAA 2761 TGATGACTTT GAAATGATAG ATTGTGACTT AGAAGATGCT ACAAAAACAC CTGACTGTTC 2821 CAGTGGACCA GTGAAAGAGG AAAGAGGTGA TCTTATAAAA TTTTACAATA CAATATATGT 2881 AGGAAGAGTG AAGTCATTTG CACTGAAATA CGACTTGGCG AATCAGGACC ATATGATGGA 2941 TGCTCCACCA CTCTCTCCTT TTCCACATAT TAAACAACAG CCAGGCTCAC CACGCCGCAT 3001 TTCCCAGCAG CACTCCATTT ATATTTCCCC GCACAAGAAT GGGTCAGGCC TTACACCAAG 3061 AAGCGCTCTG CTGTACAAGT TCAATGGCAG CCCTTCTAAG GTAAGGTGCC CAACCTTCTT 3121 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 3181 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 3241 TGGAAAGGAC GAGAAAGCGC CTCCCTATTT AAGAGCACGC GTTAAGTCga caatcaacct 3301 ctggattaca aaatttgtga aagatt