Construct: ORF TRCN0000479045
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011765.1_s317c1
- Derived from:
- ccsbBroadEn_05789
- DNA Barcode:
- CGAGCACTCCCATAGAACCCCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AP2B1 (163)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479045
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163 | AP2B1 | adaptor related protein com... | NM_001030006.2 | 99.9% | 100% | 2061G>C |
| 2 | human | 163 | AP2B1 | adaptor related protein com... | XM_005257937.4 | 99.9% | 100% | 2061G>C |
| 3 | human | 163 | AP2B1 | adaptor related protein com... | XM_005257938.3 | 99.9% | 100% | 2061G>C |
| 4 | human | 163 | AP2B1 | adaptor related protein com... | NM_001282.3 | 98.4% | 98.5% | 1989_1990ins42;2019G>C |
| 5 | human | 163 | AP2B1 | adaptor related protein com... | XM_017024284.2 | 98.4% | 98.5% | 1989_1990ins42;2019G>C |
| 6 | human | 163 | AP2B1 | adaptor related protein com... | XM_011524448.2 | 96.7% | 96.7% | 2061G>C;2453_2548del |
| 7 | human | 163 | AP2B1 | adaptor related protein com... | XM_011524449.3 | 96.7% | 96.7% | 2061G>C;2453_2548del |
| 8 | human | 163 | AP2B1 | adaptor related protein com... | XM_011524450.2 | 96.7% | 96.7% | 2061G>C;2453_2548del |
| 9 | human | 163 | AP2B1 | adaptor related protein com... | XM_011524451.2 | 95.2% | 95.3% | 1989_1990ins42;2019G>C;2411_2506del |
| 10 | human | 163 | AP2B1 | adaptor related protein com... | XM_017024285.1 | 93.9% | 94% | 0_1ins171;1890G>C |
| 11 | human | 163 | AP2B1 | adaptor related protein com... | XM_011524455.2 | 92.4% | 92.5% | 0_1ins171;1818_1819ins42;1848G>C |
| 12 | human | 163 | AP2B1 | adaptor related protein com... | XM_017024286.1 | 92.4% | 92.5% | 0_1ins171;1818_1819ins42;1848G>C |
| 13 | human | 163 | AP2B1 | adaptor related protein com... | XM_011524452.1 | 90.9% | 90.9% | 0_1ins171;1890G>C;2282_2377del |
| 14 | human | 163 | AP2B1 | adaptor related protein com... | XM_011524453.1 | 90.9% | 90.9% | 0_1ins171;1890G>C;2282_2377del |
| 15 | human | 163 | AP2B1 | adaptor related protein com... | XM_011524454.1 | 90.9% | 90.9% | 0_1ins171;1890G>C;2282_2377del |
| 16 | human | 163 | AP2B1 | adaptor related protein com... | XM_005257941.3 | 75% | 70% | (many diffs) |
| 17 | human | 163 | AP2B1 | adaptor related protein com... | XM_017024287.2 | 75% | 70% | (many diffs) |
| 18 | mouse | 71770 | Ap2b1 | adaptor-related protein com... | NM_001035854.2 | 92.5% | 99.8% | (many diffs) |
| 19 | mouse | 71770 | Ap2b1 | adaptor-related protein com... | XM_006534258.3 | 92.5% | 99.8% | (many diffs) |
| 20 | mouse | 71770 | Ap2b1 | adaptor-related protein com... | XM_017314772.1 | 92.5% | 99.8% | (many diffs) |
| 21 | mouse | 71770 | Ap2b1 | adaptor-related protein com... | NM_027915.3 | 91% | 98.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2919
- ORF length:
- 2853
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgactccaag tatttcacaa ccaataaaaa aggagaaata tttgaactaa 121 aagctgaact caacaatgaa aagaaagaaa agagaaagga ggctgtgaag aaagtgattg 181 ctgctatgac cgtggggaag gatgttagtt ctctctttcc agacgtagtg aactgtatgc 241 agactgacaa tctggaacta aagaagcttg tgtatctcta cttgatgaac tacgccaaga 301 gtcagccaga catggccatc atggctgtaa acagctttgt gaaggactgt gaagatccta 361 atcctttgat tcgagccttg gcagtcagaa ccatggggtg catccgggta gacaaaatta 421 cagaatatct ctgtgagccg ctccgcaagt gcttgaagga tgaggatccc tatgttcgga 481 aaacagcagc agtctgcgtg gcaaaactcc atgatatcaa tgcccaaatg gtggaagatc 541 agggatttct ggattctcta cgggatctca tagcagattc aaatccaatg gtggtggcta 601 atgccgtagc ggcattatct gaaatcagtg agtctcaccc aaacagcaac ttacttgatc 661 tgaacccaca gaacattaat aagctgctga cagccctgaa tgaatgcact gaatggggcc 721 agattttcat cctggactgc ctgtctaatt acaaccctaa agatgatcgg gaggctcaga 781 gcatctgtga gcgggtaact ccccggctat cccatgccaa ctcagcagtg gtgctttcag 841 cggtaaaagt cctaatgaag tttctagaat tgttacctaa ggattctgac tactacaata 901 tgctgctgaa gaagttagcc cctccacttg tcactttgct gtctggggag ccagaagtgc 961 agtatgtcgc cctgaggaac atcaacttaa ttgtccagaa aaggcctgaa atcttgaagc 1021 aggaaatcaa agtcttcttt gtgaagtaca atgatcccat ctatgttaaa ctagagaagt 1081 tggacatcat gattcgtttg gcatctcaag ccaacattgc tcaggttctg gcagaactga 1141 aagaatatgc tacagaggtg gatgttgact ttgttcgaaa agctgtgcgg gccattggac 1201 ggtgtgccat caaggtggag caatctgcag agcgctgtgt aagcacattg cttgatctaa 1261 tccagaccaa agtgaattat gtggtccaag aagcaattgt tgtcatcagg gacatcttcc 1321 gcaaataccc caacaagtat gaaagtatca tcgccactct gtgtgagaac ttagactcgc 1381 tggatgagcc agatgctcga gcagctatga tttggattgt gggagaatat gctgaaagaa 1441 ttgacaatgc agatgagtta ctagaaagct tcctggaggg ttttcacgat gaaagcaccc 1501 aggtgcagct cactctgctt actgccatag tgaagctgtt tctcaagaaa ccatcagaaa 1561 cacaggagct agtccagcag gtcttgagtt tggcaacaca ggattctgat aatcctgacc 1621 ttcgagaccg gggctatatt tattggcgcc ttctctcaac tgaccctgtt acagctaaag 1681 aagtagtctt gtctgagaag ccactgatct ctgaggagac ggaccttatt gagccaactc 1741 tgctggatga gctaatctgc cacattggtt ctttggcctc tgtgtatcat aagcctccca 1801 atgcttttgt ggaaggaagt catggaattc atcgtaaaca cttgccaatt catcatggga 1861 gcactgatgc aggtgacagc cctgttggca ctaccactgc aacgaacctg gaacagcctc 1921 aggttatccc ctctcaaggt gatcttctag gggatctttt aaaccttgac ctcggtcccc 1981 cagtcaatgt gccacaggtg tcctccatgc agatgggagc agtggatctc ctaggaggag 2041 gactagatag tctgcttggc agtgaccttg gcgggggcat tggaggaagt ccggcagtgg 2101 gacaatcctt catcccatca tcggtccctg caacctttgc tccttcacct acacctgctg 2161 tggtcagcag tggactgaat gacctgtttg aactctccac agggataggc atggcacctg 2221 gtggatatgt ggctcctaag gctgtctggc tacctgcagt aaaggctaaa ggcttggaga 2281 tttccggaac atttactcac cgccaagggc acatctatat ggaaatgaac ttcaccaata 2341 aagctctgca gcacatgaca gattttgcaa tccagtttaa caaaaatagc tttggtgtca 2401 tccccagcac tcctctggcc atccatacac cactgatgcc aaaccagagc attgatgtct 2461 ccctgcctct caataccttg ggcccagtca tgaagatgga acctctgaat aacctccagg 2521 tggctgtgaa aaacaatatc gatgtcTTCT ACTTCAGCTG CCTCATCCCA CTCAATGTGC 2581 TTTTTGTAGA AGATGGCAAA ATGGAGCGCC AGGTCTTCCT TGCAACATGG AAGGATATTC 2641 CCAATGAAAA TGAACTTCAG TTTCAGATTA AGGAATGTCA TTTAAATGCT GACACTGTTT 2701 CCAGCAAGTT GCAAAACAAC AATGTTTATA CTATTGCCAA GAGGAATGTG GAAGGGCAGG 2761 ACATGCTGTA CCAATCCCTG AAGCTCACTA ATGGCATTTG GATTTTGGCC GAACTACGTA 2821 TCCAGCCAGG AAACCCCAAT TACACGCTGT CACTGAAGTG TAGAGCTCCT GAAGTCTCTC 2881 AATACATCTA TCAGGTCTAC GACAGCATTT TGAAAAACTA CCCAACTTTC TTGTACAAAG 2941 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 3001 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 3061 ACGACGAGCA CTCCCATAGA ACCCCGCGAC GCGTTAAGTC gacaatcaac ctctggatta 3121 caaaatttgt gaaagatt