Construct: ORF TRCN0000479119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016895.1_s317c1
- Derived from:
- ccsbBroadEn_10793
- DNA Barcode:
- TCTGCCGGTATATATCTTAAGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- E2F3 (1871)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479119
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1871 | E2F3 | E2F transcription factor 3 | XR_002956265.1 | 8.8% | 1_3031del;3431_4529del | |
| 2 | human | 1871 | E2F3 | E2F transcription factor 3 | XR_001743194.2 | 8.4% | 1_3219del;3619_4717del | |
| 3 | human | 1871 | E2F3 | E2F transcription factor 3 | XR_001743195.2 | 8.3% | 1_3271del;3671_4769del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 465
- ORF length:
- 399
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCA AAGTGGTGGT GGGGTCAAGA CAGATGACAC CAGCACTTTA AACTCTTTGT 121 GTGGGTATGC GTGGGTGTAT GTTTGGGAAG AAAAACAAAG GTGCAGACTA TCTTCCTTTT 181 TTTCTTCTTC AGCCTCCATC CCTGGCCTCC TCCCCTCACA CACACTGGAC TTGGTACAAA 241 ATGTCGGTGT GGTCCTAGAT GAAGCATTGG GGTGGGGGAG GGAGAGGGAG CTTTGTGTTA 301 AGTGCCTACT GGAAATGCAC TGTGGGGTTT TTTCCTGTAT GGGAAACCAT TTATGCCAAG 361 CTTTTCCCCA TTTCCCATAT TTATCTCATC TGGTTAGCTG CCTCTGCTTC CAGCTTTGTG 421 TAATTCTCTT TGCCAGCTGC ACAAAGCTGA TTTTTTCCAA AGTCTACCCA ACTTTCTTGT 481 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 541 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 601 GAAAGGACGA TCTGCCGGTA TATATCTTAA GCCAACGCGT TAAGTCgaca atcaacctct 661 ggattacaaa atttgtgaaa gatt