Construct: ORF TRCN0000479120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016519.1_s317c1
- Derived from:
- ccsbBroadEn_00884
- DNA Barcode:
- TTAAGGAATAGATCAATCATAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ITGA5 (3678)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479120
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3678 | ITGA5 | integrin subunit alpha 5 | NM_002205.5 | 100% | 100% | |
| 2 | human | 3678 | ITGA5 | integrin subunit alpha 5 | XM_024448970.1 | 51.9% | 51.9% | 0_1ins1512 |
| 3 | mouse | 16402 | Itga5 | integrin alpha 5 (fibronect... | NM_010577.4 | 87.2% | 90.4% | (many diffs) |
| 4 | mouse | 16402 | Itga5 | integrin alpha 5 (fibronect... | NM_001314041.1 | 60.2% | 62.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3213
- ORF length:
- 3147
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gagccggacg ccagagtccc ctctccacgc cgtgcagctg cgctggggcc 121 cccggcgccg acccccgctg ctgccgctgc tgttgctgct gctgccgccg ccacccaggg 181 tcgggggctt caacttagac gcggaggccc cagcagtact ctcggggccc ccgggctcct 241 tcttcggatt ctcagtggag ttttaccggc cgggaacaga cggggtcagt gtgctggtgg 301 gagcacccaa ggctaatacc agccagccag gagtgctgca gggtggtgct gtctacctct 361 gtccttgggg tgccagcccc acacagtgca cccccattga atttgacagc aaaggctctc 421 ggctcctgga gtcctcactg tccagctcag agggagagga gcctgtggag tacaagtcct 481 tgcagtggtt cggggcaaca gttcgagccc atggctcctc catcttggca tgcgctccac 541 tgtacagctg gcgcacagag aaggagccac tgagcgaccc cgtgggcacc tgctacctct 601 ccacagataa cttcacccga attctggagt atgcaccctg ccgctcagat ttcagctggg 661 cagcaggaca gggttactgc caaggaggct tcagtgccga gttcaccaag actggccgtg 721 tggttttagg tggaccagga agctatttct ggcaaggcca gatcctgtct gccactcagg 781 agcagattgc agaatcttat taccccgagt acctgatcaa cctggttcag gggcagctgc 841 agactcgcca ggccagttcc atctatgatg acagctacct aggatactct gtggctgttg 901 gtgaattcag tggtgatgac acagaagact ttgttgctgg tgtgcccaaa gggaacctca 961 cttacggcta tgtcaccatc cttaatggct cagacattcg atccctctac aacttctcag 1021 gggaacagat ggcctcctac tttggctatg cagtggccgc cacagacgtc aatggggacg 1081 ggctggatga cttgctggtg ggggcacccc tgctcatgga tcggacccct gacgggcggc 1141 ctcaggaggt gggcagggtc tacgtctacc tgcagcaccc agccggcata gagcccacgc 1201 ccacccttac cctcactggc catgatgagt ttggccgatt tggcagctcc ttgacccccc 1261 tgggggacct ggaccaggat ggctacaatg atgtggccat cggggctccc tttggtgggg 1321 agacccagca gggagtagtg tttgtatttc ctgggggccc aggagggctg ggctctaagc 1381 cttcccaggt tctgcagccc ctgtgggcag ccagccacac cccagacttc tttggctctg 1441 cccttcgagg aggccgagac ctggatggca atggatatcc tgatctgatt gtggggtcct 1501 ttggtgtgga caaggctgtg gtatacaggg gccgccccat cgtgtccgct agtgcctccc 1561 tcaccatctt ccccgccatg ttcaacccag aggagcggag ctgcagctta gaggggaacc 1621 ctgtggcctg catcaacctt agcttctgcc tcaatgcttc tggaaaacac gttgctgact 1681 ccattggttt cacagtggaa cttcagctgg actggcagaa gcagaaggga ggggtacggc 1741 gggcactgtt cctggcctcc aggcaggcaa ccctgaccca gaccctgctc atccagaatg 1801 gggctcgaga ggattgcaga gagatgaaga tctacctcag gaacgagtca gaatttcgag 1861 acaaactctc gccgattcac atcgctctca acttctcctt ggacccccaa gccccagtgg 1921 acagccacgg cctcaggcca gccctacatt atcagagcaa gagccggata gaggacaagg 1981 ctcagatctt gctggactgt ggagaagaca acatctgtgt gcctgacctg cagctggaag 2041 tgtttgggga gcagaaccat gtgtacctgg gtgacaagaa tgccctgaac ctcactttcc 2101 atgcccagaa tgtgggtgag ggtggcgcct atgaggctga gcttcgggtc accgcccctc 2161 cagaggctga gtactcagga ctcgtcagac acccagggaa cttctccagc ctgagctgtg 2221 actactttgc cgtgaaccag agccgcctgc tggtgtgtga cctgggcaac cccatgaagg 2281 caggagccag tctgtggggt ggccttcggt ttacagtccc tcatctccgg gacactaaga 2341 aaaccatcca gtttgacttc cagatcctca gcaagaatct caacaactcg caaagcgacg 2401 tggtttcctt tcggctctcc gtggaggctc aggcccaggt caccctgaac ggtgtctcca 2461 agcctgaggc agtgctattc ccagtaagcg actggcatcc ccgagaccag cctcagaagg 2521 aggaggacct gggacctgct gtccaccatg tctatgagct catcaaccaa ggccccagct 2581 ccattagcca gggtgtgctg gaactcagct gtccccaggc tctggaaggt cagcagctcc 2641 tatatgtgac cagagttacg ggactcaact gcaccaccaa tcaccccatt aacccaaagg 2701 gcctggagtt ggatcccgag ggttccctgc accaccagca aaaacgggaa gctccaagcc 2761 gcagctctgc ttcctcggga cctcagatcc tgaaatGCCC GGAGGCTGAG TGTTTCAGGC 2821 TGCGCTGTGA GCTCGGGCCC CTGCACCAAC AAGAGAGCCA AAGTCTGCAG TTGCATTTCC 2881 GAGTCTGGGC CAAGACTTTC TTGCAGCGGG AGCACCAGCC ATTTAGCCTG CAGTGTGAGG 2941 CTGTGTACAA AGCCCTGAAG ATGCCCTACC GAATCCTGCC TCGGCAGCTG CCCCAAAAAG 3001 AGCGTCAGGT GGCCACAGCT GTGCAATGGA CCAAGGCAGA AGGCAGCTAT GGCGTCCCAC 3061 TGTGGATCAT CATCCTAGCC ATCCTGTTTG GCCTCCTGCT CCTAGGTCTA CTCATCTACA 3121 TCCTCTACAA GCTTGGATTC TTCAAACGCT CCCTCCCATA TGGCACCGCC ATGGAAAAAG 3181 CTCAGCTCAA GCCTCCAGCC ACCTCTGATG CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 3241 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3301 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 3361 AAGGAATAGA TCAATCATAG TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 3421 ttgtgaaaga tt