Construct: ORF TRCN0000479137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011221.1_s317c1
- Derived from:
- ccsbBroadEn_15899
- DNA Barcode:
- ATACAGAATGTATTGCAATAAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASIC4 (55515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479137
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55515 | ASIC4 | acid sensing ion channel su... | XM_017004439.1 | 84.9% | 76.3% | (many diffs) |
| 2 | human | 55515 | ASIC4 | acid sensing ion channel su... | NM_018674.5 | 41.3% | 37.7% | (many diffs) |
| 3 | human | 55515 | ASIC4 | acid sensing ion channel su... | NM_182847.2 | 38.5% | 34.9% | (many diffs) |
| 4 | mouse | 241118 | Asic4 | acid-sensing (proton-gated)... | NM_183022.3 | 46.7% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 954
- ORF length:
- 888
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cagtggcctg gagatcatgc tggacatcca gcaggaggag tacctgccca 121 tctggaggga gacaaatgag acgtcgtttg aggcaggtat tcgggtgcag atccacagcc 181 aggaggagcc gccctacatc caccagctgg ggttcggggt gtccccaggc ttccagacct 241 ttgtgtcctg ccaggaacag cggctgacct acctgcccca gccctggggc aactgccgcg 301 cagagagtga gctcagggag cctgagcttc agggctactc ggcctacagt gtgtctgcct 361 gccggctgcg ctgtgaaaag gaggccgtgc ttcagcgctg ccactgccgg atggtgcaca 421 tgccaggcaa tgagaccatc tgcccaccaa atatctacat cgagtgtgca gaccacacac 481 tggactccct gggtgggggc cctgagggcc cgtgcttctg ccccaccccc tgcaaccTGA 541 CACGCTATGG GAAAGAGATC TCCATGGTCA GGATCCCCAA CAGGGGCTCA GCCCGGTACC 601 TGGCGAGGAA GTACAACCGC AACGAGACCT ACATACGGGA GAACTTCCTG GTCCTAGATG 661 TCTTCTTTGA GGCCCTGACC TCTGAAGCCA TGGAGCAGCG AGCAGCCTAT GGCCTGTCAG 721 CCCTGCTGGG AGACCTCGGG GGACAGATGG GCCTGTTCAT TGGGGCCAGC ATCCTCACGT 781 TGCTGGAGAT CCTCGACTAC ATCTATGAGG CAAGGGCCCT GGAGAAGGCA GGGTGGGAGT 841 GGGGGCCGTG GGCAAAGCAG AAGGGGGCAG TGCGGGGTGC CTGGTGGAGC TGGCCTGGCT 901 GGAAAGTCAG GTTCAGGGTT CTCTGCCAGG GTCCCCTGAC TGGCTGGCAG GCCTGCCCAA 961 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1021 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1081 TATCTTGTGG AAAGGACGAA TACAGAATGT ATTGCAATAA ACTACGCGTT AAGTCgacaa 1141 tcaacctctg gattacaaaa tttgtgaaag att