Construct: ORF TRCN0000479146
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005938.2_s317c1
- Derived from:
- ccsbBroadEn_15076
- DNA Barcode:
- GTGACTGGTTATGTCGCCGGACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIX4 (51804)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479146
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51804 | SIX4 | SIX homeobox 4 | XM_005267759.2 | 98.3% | 98.3% | 1_39del |
| 2 | human | 51804 | SIX4 | SIX homeobox 4 | NM_017420.5 | 97.3% | 97.3% | 1_63del |
| 3 | human | 51804 | SIX4 | SIX homeobox 4 | XR_001750375.2 | 35.3% | 1_134del;933_1097del;2580_6450del | |
| 4 | mouse | 20474 | Six4 | sine oculis-related homeobox 4 | XM_006515632.3 | 87.5% | 88.7% | (many diffs) |
| 5 | mouse | 20474 | Six4 | sine oculis-related homeobox 4 | NM_011382.2 | 86.7% | 87.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2349
- ORF length:
- 2280
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaaagcgcc tcggaagggc aggaggcgca ccgagaagtg gcggggggcg 121 cggcggtagg gctgagcccc ccggctccag ccccttttcc cctggagccg ggggacgccg 181 cgaccgctgc cgccagggtg agcggagagg aaggggcagt ggcggcggcg gcggccggag 241 cggcggcgga tcaggtacaa ctccactcgg aacttctggg caggcaccac cacgccgccg 301 ccgccgccgc gcagaccccg ctggccttct cgcccgacca cgtcgcctgc gtgtgcgagg 361 cactgcagca ggggggcaac ctggaccgcc tggcccggtt cctgtggtcc ctgccccaga 421 gcgacctgct acgtggcaac gagagcctgc tgaaggcgcg ggcgctcgtg gccttccacc 481 agggcatcta ccccgagctc tacagcatcc tcgagagcca cagcttcgag tcggccaacc 541 acccgctgct gcagcagctc tggtacaagg cgcgctacac cgaggccgag cgagcccgcg 601 gccggccgct gggagccgta gacaagtacc ggctgcgcag gaaattcccc ctgccccgca 661 ccatctggga cggcgaggag acggtgtatt gtttcaagga gaagtcgcgc aacgcgctca 721 aggagctcta caagcagaat cgctaccctt cgcccgccga gaagcggcac ctggccaaga 781 tcaccggcct ctccctcacc caggtcagca actggttcaa gaaccgccgg cagcgcgaca 841 ggaacccctc cgagacccag tccaaaagtg agtcagatgg caaccccagc actgaagatg 901 aatccagcaa gggacatgag gatttatctc ctcacccact ctccagttca tctgatggca 961 tcaccaacct cagcctttcc agtcatatgg agccagtata tatgcaacaa attggaaatg 1021 ctaagatatc attaagctct tctggagttc tgttgaatgg aagcttggta cctgcaagta 1081 cttcacctgt cttccttaat ggaaattctt ttattcaggg acccagtgga gttatcctta 1141 atggattaaa tgtgggaaat acacaggcag tggcattgaa cccaccaaaa atgtcatcaa 1201 acattgtgag caatggtata tccatgactg acatactggg gtctacttcc caggacgtga 1261 aggaattcaa agtcctccag agttctgcta actcagcaac caccacgtcc tacagcccca 1321 gtgtccctgt ctcattccca ggcctgatac ccagcactga ggtgaaaaga gaaggcattc 1381 aaacagtggc ttcccaagat ggagggtctg tagtgacttt tactacacca gtgcaaatta 1441 accagtatgg cattgtccag atccccaatt ccggagcaaa cagccagttc cttaatggga 1501 gcattggatt ctctccactg cagctgcccc ctgtgtcagt ggcagcttca caaggtaata 1561 tctcagtaag ctcaagcact tcagatggaa gcacatttac aagtgagtct accacagtcc 1621 agcaaggaaa ggttttcttg agctctcttg ctcccagtgc agtggtatac acggttccta 1681 atacaggcca gactatagga tctgtgaaac aggaaggctt ggaaaggagc ctggtatttt 1741 ctcagttgat gcctgtcaat cagaatgcac aagtaaatgc aaacctgtct tctgaaaaca 1801 tctcggggag tggcctgcat ccactggcct cctcattagt taatgtatct ccaactcaca 1861 atttttctct cagtccctct acactactaa atcccactga gcTAAACCGC GACATTGCCG 1921 ATAGCCAACC AATGTCTGCA CCGGTGGCAA GCAAATCTAC TGTGACATCT GTCAGCAACA 1981 CTAACTATGC AACTCTTCAG AACTGCTCCC TTATTACTGG TCAAGACCTA TTGTCAGTCC 2041 CTATGACTCA GGCTGCCCTT GGGGAAATAG TTCCTACAGC TGAAGATCAG GTAGGTCACC 2101 CCTCCCCAGC AGTACATCAG GATTTTGTCC AAGAACATCG TTTGGTTCTG CAATCGGTAG 2161 CTAACATGAA AGAGAATTTC TTATCAAATT CTGAGAGCAA AGCAACAAGT AGCTTAATGA 2221 TGCTGGACTC TAAATCCAAG TATGTCTTAG ATGGCATGGT TGATACTGTC TGTGAAGACC 2281 TGGAAACAGA CAAAAAAGAG CTTGCCAAGC TCCAGACTGT CCAGCTGGAT GAAGATATGC 2341 AAGACTTATT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 2401 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2461 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGTGACT GGTTATGTCG CCGGACATAC 2521 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt