Construct: ORF TRCN0000479148
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000799.1_s317c1
- Derived from:
- ccsbBroadEn_05872
- DNA Barcode:
- TGTTTATACTCTGTAATTGCCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1B1 (525)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479148
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 525 | ATP6V1B1 | ATPase H+ transporting V1 s... | NM_001692.4 | 99.8% | 100% | 138C>T;1002C>T |
| 2 | human | 525 | ATP6V1B1 | ATPase H+ transporting V1 s... | XM_011532907.2 | 88.7% | 84.4% | (many diffs) |
| 3 | mouse | 110935 | Atp6v1b1 | ATPase, H+ transporting, ly... | NM_134157.2 | 87.4% | 93.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1605
- ORF length:
- 1539
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc catggagata gacagcaggc ctggggggct ccccggcagt agctgcaacc 121 taggtgcagc ccgagaacac atgcaggcgg tcacccgaaa ctacatcacc cacccccgtg 181 tcacctacag gactgtgtgc agtgtgaacg ggcccctggt ggtgctggac cgggtcaagt 241 ttgcccagta tgcggagatc gtccacttca ccctcccaga tgggactcag aggagcgggc 301 aggtgcttga ggtggctggc accaaggcga ttgttcaggt gtttgaaggg acatcaggga 361 tcgatgccag gaagaccact tgcgaattta caggggacat cctacgaact ccggtgtcag 421 aggacatgct gggtcgggtt ttcaatggct ccggcaagcc cattgacaag gggccagtgg 481 tcatggcgga ggactttctg gatatcaatg gccagcccat caacccgcac tcccgcatct 541 accccgagga gatgattcag acgggcattt ctcctattga cgtcatgaac agcattgccc 601 gcggccagaa gatccccatc ttctcagcag ccgggctccc ccacaatgag attgccgctc 661 agatctgccg ccaggcgggg ctggtgaaga agtccaaggc tgtgctggat taccatgacg 721 acaacttcgc catcgtcttt gcagccatgg gggtgaacat ggagacagcc agattcttca 781 agtctgactt tgagcagaat ggaaccatgg ggaacgtctg cctcttcctg aacttggcca 841 atgaccccac gatcgagcgg atcatcaccc cgcgcctggc gctgaccact gctgaattcc 901 ttgcctacca gtgtgagaag catgtgctgg tcatactgac ggacatgagt tcctatgcag 961 aggccttgcg ggaggtctct gctgctagag aggaggtgcc tgggcgccga gggtttcctg 1021 gatatatgta cacagacctg gccaccatct acgagcgggc gggccgtgtg gagggtcggg 1081 gaggatccat cacacagatc cccatcctca ccatgcccaa cgacgatatc acccacccta 1141 tcccagactT GACGGGCTTC ATCACAGAGG GACAGATCTA CGTGGACAGA CAGCTTCACA 1201 ACAGACAGAT CTACCCCCCC ATCAACGTGC TCCCTTCCCT GTCGCGGCTG ATGAAGTCAG 1261 CCATTGGGGA AGGCATGACA AGAAAGGACC ATGGAGATGT CTCCAACCAG CTGTACGCCT 1321 GCTATGCCAT CGGGAAGGAC GTGCAGGCCA TGAAGGCAGT AGTTGGGGAG GAGGCGCTCA 1381 CCTCTGAGGA CCTGCTCTAC CTGGAATTCC TGCAGAAGTT TGAGAAGAAC TTCATCAATC 1441 AGGGCCCCTA CGAGAACCGC TCGGTGTTCG AGTCGCTGGA CCTGGGCTGG AAGCTGCTGC 1501 GCATCTTCCC CAAGGAGATG CTGAAGCGCA TTCCGCAGGC CGTGATCGAC GAGTTCTATT 1561 CCCGCGAGGG GGCGCTGCAG GACCTCGCGC CTGACACTGC GCTCTACCCA ACTTTCTTGT 1621 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1681 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1741 GAAAGGACGA TGTTTATACT CTGTAATTGC CTAGACGCGT TAAGTCgaca atcaacctct 1801 ggattacaaa atttgtgaaa gatt