Construct: ORF TRCN0000479151

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017317.1_s317c1
Derived from:
ccsbBroadEn_07887
DNA Barcode:
GGTATGAACATTTCTAGCAGCTAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GCAT (23464)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000479151
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23464 GCAT glycine C-acetyltransferase NM_014291.3 99.8% 99.5% 115C>T;1159C>T
2 human 23464 GCAT glycine C-acetyltransferase XM_017028677.2 99.8% 99.5% 115C>T;1159C>T
3 human 23464 GCAT glycine C-acetyltransferase XM_005261411.2 96.6% 96.3% 115C>T;986_1027del;1201C>T
4 human 23464 GCAT glycine C-acetyltransferase NM_001171690.1 94% 93.7% 115C>T;195_272del;1237C>T
5 human 23464 GCAT glycine C-acetyltransferase XM_005261410.4 94% 93.7% 115C>T;195_272del;1237C>T
6 human 23464 GCAT glycine C-acetyltransferase XM_005261409.4 91.1% 90.8% (many diffs)
7 human 23464 GCAT glycine C-acetyltransferase XM_017028676.2 84.5% 84.2% (many diffs)
8 human 23464 GCAT glycine C-acetyltransferase XM_017028675.2 82.5% 82.2% (many diffs)
9 human 23464 GCAT glycine C-acetyltransferase XM_017028674.2 80.2% 80% (many diffs)
10 human 23464 GCAT glycine C-acetyltransferase XR_002958677.1 69.1% (many diffs)
11 human 23464 GCAT glycine C-acetyltransferase XM_024452183.1 56.9% 56.8% 0_1ins540;619C>T
12 human 23464 GCAT glycine C-acetyltransferase XM_011530023.2 55.1% 54.9% 0_1ins540;446_487del;661C>T
13 mouse 26912 Gcat glycine C-acetyltransferase... NM_013847.4 85.5% 90.2% (many diffs)
14 mouse 26912 Gcat glycine C-acetyltransferase... NM_001161712.1 77.4% 78.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1323
ORF length:
1257
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg gcctgggaac gcctggcgcg ccgcactctt ctgggtgccc cgcggccgcc
121gcgcacagtc agcgctggcc cagctgcgtg gcattctgga gggggagctg gaaggcatct
181gcggagctgg cacttggaag agtgagcggg tcatcacgtc ccgtcagggg ccgcacatcc
241gcgtggacgg cgtctccgga ggaatcctta acttctgtgc caacaactac ctgggcctga
301gcagccaccc tgaggtgatc caggcaggtc tgcaggctct ggaggagttt ggagctggcc
361tcagctctgt ccgctttatc tgtggaaccc agagcatcca caagaatcta gaagcaaaaa
421tagcccgctt ccaccagcgg gaggatgcca tcctctatcc cagctgttat gacgccaacg
481ccggcctctt tgaggccctg ctgaccccag aggacgcagt cctgtcggac gagctgaacc
541atgcctccat catcgacggc atccggctgt gcaaggccca caagtaccgc tatcgccacc
601tggacatggc cgacctagaa gccaagctgc aggaggccca gaagcatcgg ctgcgcctgg
661tggccactga tggggccttt tccatggatg gcgacatcgc acccctgcag gagatctgct
721gcctcgcctc tagatatggt gccctggtct tcatggatga atgccatgcc actggcttcc
781tggggcccac aggacggggc acagatgagc tgctgggtgt gatggaccag gtcaccatca
841tcaactccac cctggggaag gccctgggtg gagcatcagg gggctacacg acagggcctg
901ggcccctggt gtccctgctg cggcagcgcg cccggccata cctcttctcc aacagtctgc
961cacctgctgt cgttggctgc gcctccaagg ccctagatct gctgatgggG AGTAACACCA
1021TTGTCCAGTC TATGGCTGCC AAGACCCAGA GGTTCCGTAG TAAGATGGAA GCTGCTGGCT
1081TCACTATCTC GGGAGCCAGT CACCCCATCT GCCCTGTGAT GCTGGGTGAT GCCCGGCTGG
1141CCTCTCGCAT GGCGGATGAC ATGCTGAAGA GAGGCATCTT TGTCATCGGG TTCAGCTACC
1201CCGTGGTCCC CAAGGGCAAG GCCTGGATCC GGGTACAGAT CTCAGCAGTG CATAGCGAGG
1261AAGACATTGA CCGCTGCGTG GAGGCCTTCG TGGAAGTGGG GCGACTGCAC GGGGCACTGC
1321CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1381TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1441GGCTTTATAT ATCTTGTGGA AAGGACGAGG TATGAACATT TCTAGCAGCT AAACGCGTTA
1501AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)