Construct: ORF TRCN0000479153
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015393.2_s317c1
- Derived from:
- ccsbBroadEn_00814
- DNA Barcode:
- CAGCTTATAAAAGTATTGATGTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IRF8 (3394)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479153
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3394 | IRF8 | interferon regulatory factor 8 | NM_002163.4 | 100% | 100% | |
| 2 | human | 3394 | IRF8 | interferon regulatory factor 8 | NM_001363907.1 | 97.7% | 97.7% | 1_30del |
| 3 | human | 3394 | IRF8 | interferon regulatory factor 8 | NM_001363908.1 | 52.1% | 52.1% | 0_1ins612 |
| 4 | mouse | 15900 | Irf8 | interferon regulatory factor 8 | NM_001301811.1 | 85.9% | 89.6% | (many diffs) |
| 5 | mouse | 15900 | Irf8 | interferon regulatory factor 8 | NM_008320.4 | 85.9% | 89.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1347
- ORF length:
- 1278
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgtgaccgg aatggtggtc ggcggcttcg acagtggctg atcgagcaga 121 ttgacagtag catgtatcca ggactgattt gggagaatga ggagaagagc atgttccgga 181 tcccttggaa acacgctggc aagcaagatt ataatcagga agtggatgcc tccattttta 241 aggcctgggc agtttttaaa gggaagttta aagaagggga caaagctgaa ccagccactt 301 ggaagacgag gttacgctgt gctttgaata agagcccaga ttttgaggaa gtgacggacc 361 ggtcccaact ggacatttcc gagccataca aagtttaccg aattgttcct gaggaagagc 421 aaaaatgcaa actaggcgtg gcaactgctg gctgcgtgaa tgaagttaca gagatggagt 481 gcggtcgctc tgaaatcgac gagctgatca aggagccttc tgtggacgat tacatgggga 541 tgatcaaaag gagcccttcc ccgccggagg cctgtcggag tcagctcctt ccagactggt 601 gggcgcagca gcccagcaca ggcgtgccgc tggtgacggg gtacaccacc tacgacgcgc 661 accattcagc attctcccag atggtgatca gcttctacta tgggggcaag ctggtgggcc 721 aggccaccac cacctgcccc gagggctgcc gcctgtccct gagccagcct gggctgcccg 781 gcaccaagct gtatgggccc gagggcctgg agctggtgcg cttcccgccg gccgacgcca 841 tccccagcga gcgacagagg caggtgacgc ggaagctgtt cGGGCACCTG GAGCGCGGGG 901 TGCTGCTGCA CAGCAGCCGG CAGGGCGTGT TCGTCAAGCG GCTGTGCCAG GGCCGCGTGT 961 TCTGCAGCGG CAACGCCGTG GTGTGCAAAG GCAGGCCCAA CAAGCTGGAG CGTGATGAGG 1021 TGGTCCAGGT CTTCGACACC AGCCAGTTCT TCCGAGAGCT GCAGCAGTTC TATAACAGCC 1081 AGGGCCGGCT TCCTGACGGC AGGGTGGTGC TGTGCTTTGG GGAAGAGTTT CCGGATATGG 1141 CCCCCTTGCG CTCCAAACTC ATTCTCGTGC AGATTGAGCA GCTGTATGTC CGGCAACTGG 1201 CAGAAGAGGC TGGGAAGAGC TGTGGAGCCG GCTCTGTGAT GCAGGCCCCC GAGGAGCCGC 1261 CGCCAGACCA GGTCTTCCGG ATGTTTCCAG ATATTTGTGC CTCACACCAG AGATCATTTT 1321 TCAGAGAAAA CCAACAGATC ACCGTCTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACAGCTTAT 1501 AAAAGTATTG ATGTTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1561 aagatt