Construct: ORF TRCN0000479162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010258.2_s317c1
- Derived from:
- ccsbBroadEn_07343
- DNA Barcode:
- GCTTTTTACAGAAGAATATCGCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKD2L1 (9033)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479162
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9033 | PKD2L1 | polycystin 2 like 1, transi... | NM_016112.3 | 99.9% | 99.8% | 1786C>T |
| 2 | human | 9033 | PKD2L1 | polycystin 2 like 1, transi... | NM_001253837.2 | 92.3% | 91.3% | 0_1ins163;71_92del;1645C>T |
| 3 | human | 9033 | PKD2L1 | polycystin 2 like 1, transi... | XM_011540323.3 | 83.8% | 81.8% | (many diffs) |
| 4 | human | 9033 | PKD2L1 | polycystin 2 like 1, transi... | XM_017016875.2 | 83.8% | 81.8% | (many diffs) |
| 5 | human | 9033 | PKD2L1 | polycystin 2 like 1, transi... | XM_011540325.3 | 82.1% | 81.3% | (many diffs) |
| 6 | human | 9033 | PKD2L1 | polycystin 2 like 1, transi... | XM_017016876.1 | 75.8% | 73.3% | (many diffs) |
| 7 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | NM_181422.3 | 81.7% | 81.8% | (many diffs) |
| 8 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XM_006527143.3 | 78.9% | 79% | (many diffs) |
| 9 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XM_011247267.2 | 78.9% | 79% | (many diffs) |
| 10 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XM_017318220.1 | 78.9% | 79% | (many diffs) |
| 11 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XM_017318221.1 | 78.9% | 79% | (many diffs) |
| 12 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XM_017318222.1 | 78.9% | 79% | (many diffs) |
| 13 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XM_017318223.1 | 78.9% | 79% | (many diffs) |
| 14 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XM_017318224.1 | 78.9% | 79% | (many diffs) |
| 15 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XM_017318225.1 | 78.9% | 79% | (many diffs) |
| 16 | mouse | 329064 | Pkd2l1 | polycystic kidney disease 2... | XR_386483.3 | 64.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2481
- ORF length:
- 2415
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgctgtggga agtcctgagg ggcaggagct gcaaaagctg gggagtggag 121 cctgggacaa ccccgcctac agtggtcccc cttccccaca cgggacgctg agagtctgca 181 ccatctccag cacggggcct ctccagcccc aacccaagaa gcctgaagat gaaccccagg 241 agacggcata caggacccag gtgtccagct gctgcctcca tatctgtcaa ggcatcagag 301 gactttgggg aacaaccctg actgagaaca cagctgagaa ccgggaactt tatatcaaga 361 ccaccctgag ggagctgttg gtatatattg tgttcctggt ggacatctgt ctactgacct 421 atggaatgac aagctccagt gcttattact acaccaaagt gatgtctgag ctcttcttac 481 atactccatc agacactgga gtctcctttc aggccatcag cagcatggcg gacttctggg 541 attttgccca gggcccacta ctggacagtt tgtattggac caaatggtac aacaaccaga 601 gcctgggcca tggctcccac tccttcatct actatgagaa catgctgctg ggggttccga 661 ggctgcggca gctaaaggtc cgcaatgact cctgtgtggt gcatgaagac ttccgggagg 721 acattctgag ctgctatgat gtctactctc cagacaaaga agaacaactc ccctttgggc 781 ccttcaatgg cacagcgtgg acataccact cgcaggatga gttggggggc ttctcccact 841 ggggcaggct cacaagctac agcggaggtg gctactacct ggaccttcca ggatcccgac 901 agggtagtgc agaggctctc cgggcccttc aggaggggct gtggctggac aggggcactc 961 gagtggtgtt catcgacttc tcagtctaca atgccaatat caatcttttc tgtgtcctga 1021 ggctggtggt ggagtttcca gctacaggag gtgccatccc atcctggcaa atccgcacag 1081 tcaagctgat ccgctatgtc agcaactggg acttctttat cgttggctgt gaggtcatct 1141 tctgcgtctt catcttctac tatgtggtgg aagagatcct ggagctccac attcaccggc 1201 ttcgctacct cagcagcatc tggaacatac tggacctggt ggtcatcttg ctctccattg 1261 tggctgtggg cttccacata ttccgaaccc tcgaggtgaa tcggctcatg gggaagctcc 1321 tgcagcagcc aaacacgtat gcagactttg agttcctcgc cttctggcag acacagtaca 1381 acaacatgaa tgctgtcaac ctcttcttcg cctggatcaa gatattcaag tacatcagct 1441 tcaacaaaac catgacccag ctctcctcca cgctggcccg ctgtgccaag gacatcctgg 1501 gcttcgccgt catgttcttc attgttttct tcgcctatgc ccaactcggc tacctgcttt 1561 tcgggaccca agtggaaaac tttagcactt tcatcaagtg cattttcact cagttccgga 1621 taatcctcgg ggactttgac tacaatgcta tcgacaatgc caaccgcatc ctgggccctg 1681 cctactttgt cacctatgtc ttcttcgtct tcttcgtgct cctgaacatg ttcctggcca 1741 tcatcaatga cacatattca gaggtcaagg aggagctggc tggacagaag gatgagctgc 1801 aactttctga cctcctgaaa cagggctaca acaagaccct actaagactg tgtctgagga 1861 aggagagggt ttcggatgtg cagaaggtcc tgcagggtgg ggagcaggag atccagtttg 1921 aggatttcac caacacctta agggaactgg gacacgcaga gcatgaaatc actgagctca 1981 cggccacctt caccaagttt gacagagatg ggaatcgtat tctggatgag aaggaacagg 2041 aaaaaatgcg acaggacctg gaggaagaga gggtggCCCT CAACACTGAG ATTGAGAAAC 2101 TAGGCCGATC TATTGTGAGC AGCCCACAAG GCAAATCGGG TCCAGAGGCT GCCAGAGCAG 2161 GAGGCTGGGT TTCAGGAGAA GAATTCTACA TGCTCACAAG GAGAGTTCTG CAGCTGGAGA 2221 CTGTCCTGGA AGGAGTAGTG TCCCAGATTG ATGCTGTAGG CTCAAAGCTG AAAATGCTGG 2281 AGAGGAAGGG GTGGCTGGCT CCCTCCCCAG GCGTGAAGGA ACAAGCTATT TGGAAGCACC 2341 CGCAGCCAGC CCCAGCTGTG ACCCCAGACC CCTGGGGAGT CCAGGGTGGG CAGGAGAGTG 2401 AGGTTCCCTA TAAAAGAGAA GAGGAAGCCT TAGAGGAGAG GAGACTCTCC CGTGGTGAGA 2461 TTCCAACGTT GCAGAGGAGT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2521 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2581 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCTT TTTACAGAAG 2641 AATATCGCAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt