Construct: ORF TRCN0000479165
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007331.1_s317c1
- Derived from:
- ccsbBroadEn_14310
- DNA Barcode:
- CGTTTGGGAGTTTCCAGATCTTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM185A (84548)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479165
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84548 | TMEM185A | transmembrane protein 185A | NM_001174092.2 | 58.9% | 58.7% | 1_357del;871G>A |
| 2 | human | 84548 | TMEM185A | transmembrane protein 185A | NM_032508.4 | 49% | 48.8% | 1_534del;1048G>A |
| 3 | human | 84548 | TMEM185A | transmembrane protein 185A | NR_104121.2 | 22.8% | 1_277del;791G>A;794_2251del | |
| 4 | mouse | 236848 | Tmem185a | transmembrane protein 185A | XM_017318471.1 | 56.3% | 63.6% | (many diffs) |
| 5 | mouse | 236848 | Tmem185a | transmembrane protein 185A | NM_001033328.2 | 46.6% | 52.7% | (many diffs) |
| 6 | mouse | 236848 | Tmem185a | transmembrane protein 185A | XM_006527981.2 | 42.9% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ctttctgtgc ctggtggtcc tctactacat tgtgtggtcc gtcttgttct 121 tgcgctctat ggatgtgatt gcggagcagc gcaggacaca cataaccatg gccctgagct 181 ggatgaccat cgtcgtgccc cttcttacat ttGAGATTCT GCTGGTTCAC AAACTGGATG 241 GCCACAACGC CTTCTCCTGC ATCCCGATCT TTGTCCCCCT TTGGCTCTCG TTGATCACGC 301 TGATGGCAAC CACATTTGGA CAGAAGGGAG GAAACCACTG GTGGTTTGGT ATCCGCAAAG 361 ATTTCTGTCA GTTTCTGCTT GAAATCTTCC CATTTCTACG AGAATATGGA AACATTTCCT 421 ATGATCTCCA TCACGAAGAT AATGAAGAAA CCGAAGAGAC CCCAGTTCCG GAGCCCCCTA 481 AAATCGCACC CATGTTTCGA AAGAAGGCCA GGGTGGTCAT TACCCAGAGC CCTGGGAAGT 541 ATGTGCTCCC ACCTCCCAAA TTAAATATCG AAATGCCAAA TTACCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGACGT TTGGGAGTTT CCAGATCTTG GACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t